Effects of RNA on the developmental potentiality of the posterior primitive streak of the chick blastoderm.

Sanyal, S.; Niu, M. C.

doi:10.1073/pnas.55.4.743

Cited by 14 publications

(1 citation statement)

References 13 publications

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“…The fact that L-RNA can indeed stimulate gut formation is a step forward in our studv on the possibility of the directed formation of specific tissue (Hillman andNiu. 1963: Niu andLeikola, 1966;Sanyal and Niu, 1966). Should L-RNA carry the message of the liver (Niu, Cordova and Niu, 1961;Zimmerman, Zoller and Turba, 1963), one immediate question is the condition under which the development of gut and liver can separately be achieved.…”

Section: Discussionmentioning

confidence: 99%

The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

Niu¹,

Leikola²

1968

The Biological Bulletin

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Section: Discussionmentioning

confidence: 99%

The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

Niu¹,

Leikola²

1968

The Biological Bulletin

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Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Lee

Kalmus

1975

J. Exp. Zool.

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The inducing capacity of sulfhydryl (SH)-containing amino acids (cysteine and glutathione) on post-nodal pieces (PNPs) of stage 4 chick blastoderms was investigated. PNPs were treated for different lengths of time with chick Ringer's solution (control group) or chick Ringer's solution containing cysteine or glutathione, followed by culturing for 2-10 days on Spratt-Haas agar medium or on the chorioallantoic membrane of 8-day chick embryos. Control PNPs rarely showed differentiation, but those treated with the amino acids for six hours or longer developed structures such as neural tissue, notochord, somite mesoderm, and nephric tubules. The pulsatile tissue was only seen in the PNPs cultured for four days or longer. Two-four hours of treatment was too short to provoke induction in a statistically significant number of PNPs. The highest frequency of induction was noted in those pretreated with glutathione (8 mug/ml) for eight hours, followed by culturing for four days. The magnitude of the inducing capacity and toxicity of the amino acids were concentration dependent: a deleterious effect was observed at 14 mug/ml; the highest frequency of induction occurred at 8 mug/ml, but the frequency decreased as the concentration decreased; at 2 mug/ml all PNPs remained viable, but only a few (9-14%) showed differentiation. The inducing capacity of the amino acids was counteracted by equimolar concentrations of rho-chloromercuribenzoic acid or omega-chloroacetophenone. The effects of glutathione (8 mug/ml) differed from those of Hensen's node grafts in that the former caused sublethal cytolysis and inhibited H-3-uridine uptake in competent ectodermal cells during the first 18 hours of cultivation.

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Induction of Differentiated Activity of the Genes by Cytoplasmic and Chromosomal Factors and Its Role in the General Organization of Morphogenesis

Medvedev¹

1970

Molecular-Genetic Mechanisms of Development

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Effects of RNA on the developmental potentiality of the posterior primitive streak of the chick blastoderm.

Cited by 14 publications

References 13 publications

The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

The Developmental Potentiality of the Liver-Rna-Treated Posterior Primitive Streak in the Chick Embryo

Studies on cell differentiation: Inducing capacity of sulfhydryl‐containing amino acids on post‐nodal pieces of chick blastoderms

Induction of Differentiated Activity of the Genes by Cytoplasmic and Chromosomal Factors and Its Role in the General Organization of Morphogenesis

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