Effects of quercitrin on bacterial translocation in a rat model of experimental colitis

Dönder, Yunus; Arıkan, Tamer; Baykan, Mehmet; Akyüz, Muhammet; Öz, Abdullah Bahadır

doi:10.1016/j.asjsur.2017.12.002

Cited by 20 publications

(10 citation statements)

References 22 publications

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“…In the present study, C. rodentium -infected mice showed significantly increased levels of proinflammatory cytokines IL-6, TNF-α, and IL-17 in the colonic mucosal tissues compared with the CTRL mice (not infected). It has been demonstrated that quercetin protects against colonic damage linked to increases in the TNF-α level (Donder et al., 2018; Ju et al., 2018). In the present study, quercetin supplementation reduced localized production of inflammatory cytokines, which in turn promoted alterations in bacterial flora composition associated with rapid repair.…”

Section: Discussionmentioning

confidence: 99%

Dietary Quercetin Increases Colonic Microbial Diversity and Attenuates Colitis Severity in Citrobacter rodentium-Infected Mice

2019

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Disturbed balance between microbiota, epithelial cells, and resident immune cells within the intestine contributes to inflammatory bowel disease (IBD) pathogenesis. The Citrobacter rodentium -induced colitis mouse model has been well documented. This model allows the analysis of host responses to enteric bacteria and facilitates improved understanding of the potential mechanisms of IBD pathogenesis. The current study evaluated the effects of dietary 30 mg/kg quercetin supplementation on C. rodentium -induced experimental colitis in C57BL/6 mice. Following dietary quercetin supplementation, the mice were infected with 5 × 10 8 CFU C. rodentium , and the pathological effects of C. rodentium were measured. The results showed that quercetin alleviated the effects of C. rodentium -induced colitis, suppressed the production of pro-inflammatory cytokines, such as interleukin (IL)-17, tumor necrosis factor alpha, and IL-6 ( p < 0.05), and promoted the production of IL-10 in the colon tissues ( p < 0.05). Quercetin supplementation also enhanced the populations of Bacteroides , Bifidobacterium , Lactobacillus, and Clostridia and significantly reduced those of Fusobacterium and Enterococcus ( p < 0.05). These findings indicate that dietary quercetin exerts therapeutic effects on C. rodentium -induced colitis, probably due to quercetin’s ability to suppress pro-inflammatory cytokines and/or modify gut microbiota. Thus, these results suggest that quercetin supplementation is effective in controlling C. rodentium -induced inflammation.

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Section: Discussionmentioning

confidence: 99%

Dietary Quercetin Increases Colonic Microbial Diversity and Attenuates Colitis Severity in Citrobacter rodentium-Infected Mice

2019

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“…Additionally, a decrease in BT to liver, spleen, and MLNs was seen after administration of 5 mg/kg/day of quercitrin in the same animal model of intestinal inflammation. This effect was possibly due to the anti-inflammatory properties of the investigated compound, as in the experiment, researchers observed a decrease in TNF-α serum concentration and MPO activity [ 127 ]. Many phytochemicals also exert antioxidant activity, which reduces oxygen-free radical levels and prevents oxidative cellular damage.…”

Section: Dietary Supplements As a Potential Preventative Agents In Btmentioning

confidence: 99%

Preventing Bacterial Translocation in Patients with Leaky Gut Syndrome: Nutrition and Pharmacological Treatment Options

Twardowska

Makaro

Binienda

et al. 2022

IJMS

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Leaky gut syndrome is a medical condition characterized by intestinal hyperpermeability. Since the intestinal barrier is one of the essential components maintaining homeostasis along the gastrointestinal tract, loss of its integrity due to changes in bacterial composition, decreased expression levels of tight junction proteins, and increased concentration of pro-inflammatory cytokines may lead to intestinal hyperpermeability followed by the development of gastrointestinal and non-gastrointestinal diseases. Translocation of microorganisms and their toxic metabolites beyond the gastrointestinal tract is one of the fallouts of the leaky gut syndrome. The presence of intestinal bacteria in sterile tissues and distant organs may cause damage due to chronic inflammation and progression of disorders, including inflammatory bowel diseases, liver cirrhosis, and acute pancreatitis. Currently, there are no medical guidelines for the treatment or prevention of bacterial translocation in patients with the leaky gut syndrome; however, several studies suggest that dietary intervention can improve barrier function and restrict bacteria invasion. This review contains current literature data concerning the influence of diet, dietary supplements, probiotics, and drugs on intestinal permeability and bacterial translocation.

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“…By activating extracellular signal-regulated kinase (ERK) signaling, luteolin may moderate colon tissue damage and reduce inflammation, apoptosis, and autophagy ( Vukelic et al, 2020 ). Luteolin treatment can decrease the activation of NF-κB and production of IL-17 and IL-23, leading to reduced colonic damage in UC rats ( Donder et al, 2018 ). Hyndarin is a compound extracted from corydalis with analgesic and antispasmodic properties, which has the highest concentration in the small intestine 1 hour after administration and effectively relieves spastic pain in UC ( Xiao, 2016 ; Qiu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology and Molecular Docking Analysis on Molecular Mechanism of Qingzi Zhitong Decoction in the Treatment of Ulcerative Colitis

et al. 2022

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Ulcerative colitis (UC) is a disease with complex pathological mechanisms. We explored the potential molecular mechanisms behind the therapeutic functions of Qingzi Zhitong decoction (QZZTD) in the treatment of UC by network pharmacology and molecular docking. QZZTD is a formula of Chinese traditional medicine consisting of 10 herbs. The potential active ingredients of QZZTD and their target genes were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database, and UC-related target genes were obtained from GeneCards and OMIM databases. A total of 138 co-identified target genes were obtained by plotting the intersection target Venn diagram, and then the STRING database and Cytoscape software were used to establish protein–protein interaction networks and herb–ingredient–target networks. Four key active compounds and nine key proteins were identified. Then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses showed that the biological functions of potential target genes were associated with DNA transcription, signaling receptor and ligand activity, cytokine activity, cellular autophagy, and antioxidant pathways, with related pathways involving the phosphatidylinositol 3-kinase (PI3K)–Akt signaling pathway, advanced glycosylation end product (AGE)–RAGE signaling pathway, tumor necrosis factor (TNF) signaling pathway, and IL-17 signaling pathway. Moreover, the binding activities of key target genes and essential active compounds of Chinese herbal medicines in QZZTD were further validated by molecular docking. This demonstrated that quercetin, luteolin, hyndarin, and beta-sitosterol had good binding to eight key proteins, and Akt1 was the target protein with the best binding activity, suggesting that Akt1 could be the essential mediator responsible for signaling transduction after QZZTD administration. The rat experiment verified that QZZTD inhibited PI3K-Akt pathway activation and reduced inflammation in UC. In conclusion, our study suggested four potential key active components, including quercetin, were identified in QZZTD, which could interact with Akt1 and modulate the activation of the PI3K-Akt pathway. The other three pathways may also be involved in the signaling transduction induced by QZZTD in the treatment of UC.

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Effects of quercitrin on bacterial translocation in a rat model of experimental colitis

Abstract: In the experimental colitis model established by using DSS, treatment with quercitrin resulted in a histopathological improvement and reduction in biochemical parameters, inflammation and in bacterial translocation (p < 0.05).

Cited by 20 publications

References 22 publications

Dietary Quercetin Increases Colonic Microbial Diversity and Attenuates Colitis Severity in Citrobacter rodentium-Infected Mice

Dietary Quercetin Increases Colonic Microbial Diversity and Attenuates Colitis Severity in Citrobacter rodentium-Infected Mice

Preventing Bacterial Translocation in Patients with Leaky Gut Syndrome: Nutrition and Pharmacological Treatment Options

Network Pharmacology and Molecular Docking Analysis on Molecular Mechanism of Qingzi Zhitong Decoction in the Treatment of Ulcerative Colitis

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