Effects of purified exosome product on rotator cuff tendon-bone healing in vitro and in vivo

Ren, Ye; Zhang, Shuwei; Wang, Yicun; Jacobson, Daniel S.; Reisdorf, Ramona L.; Kuroiwa, Tomoyuki; Behfar, Atta; Moran, Steven L.; Steinmann, Scott P.; Zhao, Chunfeng

doi:10.1016/j.biomaterials.2021.121019

Cited by 38 publications

(69 citation statements)

References 43 publications

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“…Mechanical strength is an indicator of tendon healing and functional outcomes [43,44,60,61]. A recent study showed improved biomechanical properties in a repaired rotator cuff model with treatment with exosome-embedded fibrin sealant [62]. However, the effects of ADSC-EVs on repaired tendons have rarely been discussed.…”

Section: Evs Have Been Generally Accepted As a Modality Of Cell-to-cell Communication Formentioning

confidence: 99%

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Chen

Lin

et al. 2021

IJMS

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Healing of ruptured tendons remains a clinical challenge because of its slow progress and relatively weak mechanical force at an early stage. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have therapeutic potential for tissue regeneration. In this study, we isolated EVs from adipose-derived stem cells (ADSCs) and evaluated their ability to promote tendon regeneration. Our results indicated that ADSC-EVs significantly enhanced the proliferation and migration of tenocytes in vitro. To further study the roles of ADSC-EVs in tendon regeneration, ADSC-EVs were used in Achilles tendon repair in rabbits. The mechanical strength, histology, and protein expression in the injured tendon tissues significantly improved 4 weeks after ADSC-EV treatment. Decorin and biglycan were significantly upregulated in comparison to the untreated controls. In summary, ADSC-EVs stimulated the proliferation and migration of tenocytes and improved the mechanical strength of repaired tendons, suggesting that ADSC-EV treatment is a potential highly potent therapeutic strategy for tendon injuries.

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Section: Evs Have Been Generally Accepted As a Modality Of Cell-to-cell Communication Formentioning

confidence: 99%

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Chen

Lin

et al. 2021

IJMS

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“…Promisingly, this novel engineered exosomal patch can stably release particles over two weeks [ 74 ]. More organized and denser collagenous tissue and fewer inflammatory cells were also displayed at the tendon-bone interface after rotator cuff repair in the TISSEEL–PEP group [ 90 ]. Wellings et al demonstrated significantly fewer external adhesions and improved mechanical functions with the application of PEP loaded onto a collagen scaffold, indicating that the off-the-shelf PEP product favored intrinsic healing instead of extrinsic healing [ 91 ].…”

Section: Exosomes In Tendon and Repair And Regenerationmentioning

confidence: 99%

“…As we can see from this review, the development of exosomal treatments is still at a very early stage of research, and little clinical application has been accomplished. One revolutionary advance is the newly developed PEP from Mayo Clinic ( Table 1 ), which has already undergone clinical trials (human; in phase I clinical trial; ClinicalTrials.gov: NCT04664738, , accessed date: 16 March 2021) in tendon repair [ 74 , 89 , 90 ], peripheral nerve regeneration [ 184 ], vaginal tissue regeneration [ 211 ], and myocardial infarction recovery (human; in phase I clinical trial; ClinicalTrials.gov: NCT04327635, , accessed date: 2 November 2021).…”

Section: Future Directionsmentioning

confidence: 99%

The Therapeutic Potential of Exosomes in Soft Tissue Repair and Regeneration

Wan

Hussain

Behfar

et al. 2022

IJMS

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Soft tissue defects are common following trauma and tumor extirpation. These injuries can result in poor functional recovery and lead to a diminished quality of life. The healing of skin and muscle is a complex process that, at present, leads to incomplete recovery and scarring. Regenerative medicine may offer the opportunity to improve the healing process and functional outcomes. Barriers to regenerative strategies have included cost, regulatory hurdles, and the need for cell-based therapies. In recent years, exosomes, or extracellular vesicles, have gained tremendous attention in the field of soft tissue repair and regeneration. These nanosized extracellular particles (30–140 nm) can break the cellular boundaries, as well as facilitate intracellular signal delivery in various regenerative physiologic and pathologic processes. Existing studies have established the potential of exosomes in regenerating tendons, skeletal muscles, and peripheral nerves through different mechanisms, including promoting myogenesis, increasing tenocyte differentiation and enhancing neurite outgrowth, and the proliferation of Schwann cells. These exosomes can be stored for immediate use in the operating room, and can be produced cost efficiently. In this article, we critically review the current advances of exosomes in soft tissue (tendons, skeletal muscles, and peripheral nerves) healing. Additionally, new directions for clinical applications in the future will be discussed.

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“…Cell therapy involves injecting cells from other body parts or allogeneic sources into the injured site [ 175 ]. Currently, research in this field focuses on differentiated cells (tendon cells and fibroblasts) and stem cells ( Table 2 ), the main cells constituting the ECM [ 176 ]. Tendon cells and fibroblasts can produce rich growth factors to promote the healing of injured sites, and they are also the most common cells in tendon tissue [ 177 ].…”

Section: Cells That Repair Tendons and Ligamentsmentioning

confidence: 99%

Functional biomaterials for tendon/ligament repair and regeneration

Tang

Wang

Xiang

et al. 2022

Regenerative Biomaterials

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With an increase in life expectancy and the popularity of high-intensity exercise, the frequency of tendon and ligament injuries has also increased. Owing to the specificity of its tissue, the rapid restoration of injured tendons and ligaments is challenging for treatment. This review summarises the latest progress in cells, biomaterials, active molecules, and construction technology in treating tendon/ligament injuries. The characteristics of supports made of different materials and the development and application of different manufacturing methods are discussed. The development of natural polymers, synthetic polymers, and composite materials has boosted the use of scaffolds. In addition, the development of electrospinning and hydrogel technology has diversified the production and treatment of materials. First, this paper briefly introduces the structure, function, and biological characteristics of tendons/ligaments. Then, it summarises the advantages and disadvantages of different materials, such as natural polymer scaffolds, synthetic polymer scaffolds, composite scaffolds, and ECM-derived biological scaffolds, in the application of tendon/ligament regeneration. We then discuss the latest applications of electrospun fiber scaffolds and hydrogels in regeneration engineering. Finally, we discuss the current problems and future directions in the development of biomaterials for restoring damaged tendons and ligaments.

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Effects of purified exosome product on rotator cuff tendon-bone healing in vitro and in vivo

Cited by 38 publications

References 43 publications

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

The Therapeutic Potential of Exosomes in Soft Tissue Repair and Regeneration

Functional biomaterials for tendon/ligament repair and regeneration

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