Effects of probiotics on haematology and biochemical parameters in mice

Salahuddin,; Akhter, Hafiza; Akter, Salma; Miah, Md. Idris; Ahmad, Nazim

doi:10.3329/bvet.v30i1.16281

Cited by 16 publications

(15 citation statements)

References 14 publications

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“…Because the initial levels exceeded the common physiological range (150-190 g/L), it is difficult to make a conclusion as to the real direction of chlorophyll effects. In addition, 7 dogs of the LF group had increased haemoglobin levels 14 days after application of L. fermentum CCM 7421, and a similar dose-dependent effect in mice has been reported (Salahuddin 2013).…”

Section: Discussionmentioning

confidence: 61%

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Strompfová

Kubašová

Farbáková

et al. 2015

Appl Microbiol Biotechnol

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Chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. Although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. Our aim was to test the growth characteristics of canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in the presence of different concentrations of chlorophyllin in vitro. Antimicrobial activity of chlorophyllin against canine indicator bacteria was also detected. In the in vivo study, chlorophyllin, L. fermentum CCM 7421 and the combination of both additives on faecal microbiota, faecal organic acid concentrations, haematological and immunological parameters in dogs were tested. Forty dogs were divided into 4 treatment groups; control (C); receiving chlorophyllin (60 mg/day/dog, CH group); L. fermentum CCM 7421 (10(8) CFU/day/dog, LF group); and both additives (CH + LF group), 10 dogs in each group. The experiment lasted for 28 days with a 14-day treatment period (sample collection at days 0, 7, 14 and 28). Results showed no growth inhibition of strain CCM 7421 by 0.05-0.25 % of chlorophyllin in broth after 24 h. Reduced growth of staphylococci, Listeria monocytogenes and Citrobacter freundii was observed at 1 % chlorophyllin (P < 0.05). In dogs, lower coliform bacteria numbers and higher concentration of propionic acid in faeces of the CH group during the treatment compared to baseline were detected (P < 0.01). Phagocytic activity of leukocytes was stimulated in all three treated groups of dogs (P < 0.05).

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Section: Discussionmentioning

confidence: 61%

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Strompfová

Kubašová

Farbáková

et al. 2015

Appl Microbiol Biotechnol

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“…Penelitian oleh Astawan et al (2011), pada kelompok tikus yang diinfeksi Enteropathogensis E. coli (EPEC) menunjukan bahwa kelompok tikus yang diberi diet probiotik memiliki jumlah sel darah merah yang lebih tinggi dibandingkan dengan kelompok tikus yang hanya diberi diet standar. Sementara pada penelitian Salahuddin et al (2013), menunjukan bahwa terjadi perubahan yang signifikan pada parameter sel darah merah yaitu peningkatan jumlah eritrosit dan konsentrasi hemoglobin pada kelompok tikus yang diberikan diet probiotik dibandingkan dengan kelompok yang tidak mendapat diet probiotik. 6,14 Sebagian besar penelitian tentang pengaruh probiotik berfokus pada pencegahan dan terapi penyakit gastrointestinal, padahal probiotik memiliki potensi dalam mencegah perluasan infeksi dan berpengaruh pada konsentrasi hemoglobin.…”

Section: Pendahuluanunclassified

“…Sementara pada penelitian Salahuddin et al (2013), menunjukan bahwa terjadi perubahan yang signifikan pada parameter sel darah merah yaitu peningkatan jumlah eritrosit dan konsentrasi hemoglobin pada kelompok tikus yang diberikan diet probiotik dibandingkan dengan kelompok yang tidak mendapat diet probiotik. 6,14 Sebagian besar penelitian tentang pengaruh probiotik berfokus pada pencegahan dan terapi penyakit gastrointestinal, padahal probiotik memiliki potensi dalam mencegah perluasan infeksi dan berpengaruh pada konsentrasi hemoglobin. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh probiotik (Lactobacillus spp.)…”

Section: Pendahuluanunclassified

“…Pemberian probiotik mampu melindungi epitel usus dari kerusakan akibat infeksi mikroba sehingga mengoptimalkan penyerapan zat besi (Fe), dan mengoptimalkan pemenuhan vitamin B12 dan asam folat yang penting dalam pembentukan dan pematangan sel darah merah dan hemoglobin. Hasil ini didukung oleh hasil penelitian Salahuddin et al (2013) dan Astawan et al (2011) yang menunjukan bahwa terdapat perbedaan konsentrasi hemoglobin yang bermakna antara kelompok yang diberi diet probiotik dibandingkan dengan kelompok yang tidak diberi diet probiotik. 6,14 Keterbatasan penelitian ini adalah tidak melakukan pemberian probiotik dengan dosis yang berbeda untuk melihat perbedaan respons imun dan perbedaan respons pemenuhan nutrisi untuk pembentukan hemoglobin.…”

Section: Pembahasanunclassified

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Probiotik Meningkatkan Konsentrasi Hemoglobin Pada Tikus Putih Yang Diinduksi Lipopolisakarida Escherichia Coli

Sari

Wihastuti

Ardiansyah³

2018

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AbstrakInfeksi bakteri Escherichia coli (E. coli) dapat menyebabkan gangguan sistem hematologi, di antaranya berupa penurunan konsentrasi hemoglobin. Konsumsi probiotik dianggap sebagai salah satu upaya untuk meningkatkan sintesis hemoglobin. Penelitian ini bertujuan untuk mengetahui pengaruh Lactobacillus spp. terhadap konsentrasi hemoglobin pada tikus putih (Rattus norvegicus) yang diinduksi oleh lipopolisakarida (LPS) E. coli. Rancangan penelitian ini adalah true experimental dengan desain randomized post test only control group. Dua puluh satu ekor tikus putih dibagi ke dalam tiga kelompok yaitu: (1) kelompok tikus tanpa perlakuan sebagai kontrol negatif (n = 7), (2) kelompok tikus yang diinduksi LPS E. coli (n = 7), dan (3) kelompok tikus yang dipapar Lactobacillus spp. dan diinduksi LPS E. coli (n = 7). Konsentrasi hemoglobin diukur dengan metode oksihemoglobin. Hasil penelitian menunjukkan perbedaan konsentrasi hemoglobin antara kelompok tikus dengan paparan probiotik dan diinduksi LPS E. coli (8,9±0,3 mmol/L) dibandingkan dengan kelompok kontrol negatif (8,0±0,8 mmol/L), dan kelompok yang hanya diinduksi LPS E. coli (8,4±0,5 mmol/L) (p < 0,05). Disimpulkan bahwa Lactobacillus spp. dapat meningkatkan konsentrasi hemoglobin pada tikus putih yang diinduksi LPS E. coli. AbstractInfection caused by E. coli may lead to a hematological system disorder, such as decreasing of hemoglobin concentration. Consumption of probiotic was considered as an effort to improve hemoglobin synthesis. This study aimed to assess the effect of Lactobacillus spp. on hemoglobin concentration in rats (Rattus norvegicus) induced by lipopolysaccharide (LPS) of E. coli. A true experimental research with randomized post test only control group design was performed. Twenty one rats were divided into three groups including: (1) a negative control (n = 7), (2) a group was induced with LPS of E. coli (n = 7), and (3) a group was exposed to Lactobacillus spp. prior to induction by LPS of E. coli (n = 7). Hemoglobin concentration was measured by oxyhemoglobin method. The results showed the difference of hemoglobin concentration among groups of rat exposed to Lactobacillus spp prior to induction by LPS of E. coli (8,9±0,3 mmol/L), control negative group (8,0±0,8 mmol/L), and group of rat induced by LPS E. coli (8,4±0,5 mmol/L) (p < 0,05). It is concluded that Lactobacillus spp. can increase the hemoglobin concentration in rats induced by LPS of E. coli.

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“…Kinal et al (2007) suggested that yeast cells also improved the digestibility and absorption of minerals such as phosphorus, magnesium, calcium, copper, potassium, zinc, and manganese. Study about the effect of probiotic on hematology was reported in mice and result showed that probiotic increased hematological parameters and lipid profile (Salahudin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Nutrient Digestibility and Performances of Frisian Holstein Calves Fed with Pennisetum purpureum and Inoculated with Buffalo’s Rumen Bacteria

Prihantoro

Evvyernie

Suryani

et al. 2014

Med Pet

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Buffalo's rumen bacteria (BRB) are potential in digesting fiber feed. BRB already adapted well with low quality forages and agricultural byproducts. The aim of this study was to determine the effect of buffalo's rumen bacteria (BRB) consortium inoculated into preweaning Frisian Holstein calves on nutrient digestibility, physiological status, mineral uptake, and blood profile. This study used 14 isolates of bacteria isolated from rumen fluid of four local buffalos. The research units consisted of seven Frisian Holstein calves at two weeks old with the average body weight of 43.6±4.5 kg. Calves were inoculated with 20 mL of buffalo's rumen bacteria isolates [4.56 x 10 9 cfu/mL] every morning for 10 weeks. The calves were divided into two groups i.e., three calves received bacterial inoculation and four calves without any inoculation. The variables which were analyzed in the preweaning and weaning period were feed intake, digestibility, average daily gain (ADG), feed conversion ratio (FCR), rumen fermentation characteristics, body weight, physiological status, blood profile, and mineral status. Data were analyzed statistically using t-test. The results showed that inoculation of buffalo's rumen bacteria into Frisian Holstein calves effectively increased feed intake, characteristics of leukocytes and neutrophils, and cobalt (Co) uptake during the weaning period. Inoculation of rumen bacteria improved rumen pH during preweaning and weaning periods. Inoculation of rumen bacteria also had no negative effects on digestibility, feed conversion (FCR), average daily gain (ADG), and physiological status.Key words: buffalo rumen bacteria, digestibility, physiological status, blood profile, Pennisetum purpureum ABSTRAK Bakteri rumen kerbau (BRK) merupakan bakteri yang mempunyai potensi tinggi dalam pemanfaatan pakan sumber serat. Bakteri ini telah teradaptasi dengan baik terhadap pakan hijauan dan limbah pertanian yang berkualitas rendah. Inokulasi bakteri rumen kerbau pada pedet memungkinkan rumen berkembang lebih awal dan fermentasi di dalam rumen lebih optimal. Penelitian ini bertujuan untuk mengetahui efektivitas inokulasi bakteri asal rumen kerbau pada pedet Frisian pada kecernaan pakan, status fisiologis, serapan mineral, dan profil darah. Penelitian ini menggunakan 14 isolat bakteri yang berasal dari cairan rumen empat jenis kerbau lokal. Penelitian terdiri atas tujuh ekor pedet prasapih jenis Frisian Holstein berumur dua minggu dengan rataan bobot badan 43,6±4,5 kg. Rumen pedet diinokulasi 20 mL konsorsium bakteri dalam bentuk dadih setiap pagi hari selama 10 minggu dengan cara dicekok menggunakan spoit pada konsentrasi bakteri 4,56 x 10 9 cfu/mL. Tiga ekor pedet diberi inokulan bakteri dan empat ekor sebagai kontrol. Peubah yang dianalisis pada penelitian periode prasapih dan pascasapih antara lain konsumsi pakan, kecernaan, pertambahan bobot badan, konversi pakan, karakteristik fermentasi, bobot badan, status fisiologi, profil darah, dan status mineral. Data dianalisis menggunakan uji-t. Hasil penelitian men...

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Effects of probiotics on haematology and biochemical parameters in mice

Cited by 16 publications

References 14 publications

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Probiotik Meningkatkan Konsentrasi Hemoglobin Pada Tikus Putih Yang Diinduksi Lipopolisakarida Escherichia Coli

Nutrient Digestibility and Performances of Frisian Holstein Calves Fed with Pennisetum purpureum and Inoculated with Buffalo’s Rumen Bacteria

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