Effects of pomegranate aril juice and its punicalagin on some key regulators of insulin resistance and oxidative liver injury in streptozotocin-nicotinamide type 2 diabetic rats

El-Beih, Nadia M.; Ramadan, Gamal; El-Husseiny, Enas A; Hussein, Ashraf S.

doi:10.1007/s11033-019-04813-8

Cited by 24 publications

(21 citation statements)

References 41 publications

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“…This effect may be influenced by the observed notable presence of ferulic acid, caffeic acid, and gallic acid in the ACE (Figure 1 and Table 2), as well as punicalagin and derivatives, which have been reported as a major constituent of P. granatum fruit peel acetone extract (Abid et al, 2017). While these phenolic have been reported to suppress lipid peroxidation and free radical production on iron‐ and diabetes‐induced oxidative stress (Balasubashini, Rukkumani, Viswanathan, & Menon, 2004; Kristinová, Mozuraityte, Storrø, & Rustad, 2009; Punithavathi, Stanely Mainzen Prince, Kumar, & Selvakumari, 2011), a recent study has shown that punicalagin reduces hepatic lipid peroxidation in type 2 diabetic rats (El‐Beih, Ramadan, El‐Husseiny, & Hussein, 2019).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the in vitro ⍺‐amylase inhibitory, antiglycation, and antioxidant properties ofPunica granatumL. (pomegranate) fruit peel acetone extract and its effect on glucose uptake and oxidative stress in hepatocytes

2020

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Punica granatum L. (pomegranate) is a widely eaten fruit. The antidiabetic, antioxidative, and antilipidemic properties of the hydroalcoholic extracts of the different plant's parts have been extensively studied, with scarce information on the acetone extract (ACE). This study investigated antidiabetic, antioxidative, and antiobesogenic properties of ACE of the fruit peel. Preliminary data showed that ACE showed stronger antioxidant (radical‐scavenging IC50 = 1.56 μg/ml) and ⍺‐amylase inhibitory (IC50 = 10.6 μg/ml) properties than the hydroalcoholic extracts and Acarbose. The ACE inhibited protein glycation and lipase activity. In hepatocytes, ACE impaired oxidative stress‐induced lipid peroxidation and reduced glutathione depletion but increased glucose uptake without decreasing the cell viability. HPLC analysis showed predominant presence of bioactive phenolic acids (ferulic, caffeic, and gallic acids) in this extract. This study suggests that ACE of P. granatum fruit peel may be an understudied extract that contains potent antidiabetic and antioxidative bioactive principles with minimal toxicity. Practical applications Plant derived medicines have been an affordable and effective alternative therapy for many metabolic diseases, including diabetes. The fruit juice and fruits of pomegranate is widely consumed for the palatable taste and cardiovascular benefits. This study provides preliminary experimental evidences confirming that of the acetone extracts of pomegranate fruit peel, which has been sparsely studied, may possess more potent antidiabetic and antioxidative property than to the routinely studied hydroalcoholic counterparts. Additionally, the acetone extract is rich in bioactive phenolic acids, suggesting that the acetone extract of pomegranate fruit peel may be a promising candidate for further antidiabetic study and a source of bioactive principles for the management of diabetes and oxidative complications.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the in vitro ⍺‐amylase inhibitory, antiglycation, and antioxidant properties ofPunica granatumL. (pomegranate) fruit peel acetone extract and its effect on glucose uptake and oxidative stress in hepatocytes

2020

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“…Pomegranate and PCG have been associated with various biological activities, including anti‐obesity, anti‐inflammatory, and anti‐diabetic effects . However, studies on the effect of PCG on obesity‐related pathogenesis and its mechanism are limited.…”

Section: Discussionmentioning

confidence: 99%

“…Pomegranate and PCG have been associated with various biological activities, including anti-obesity, anti-inflammatory, and anti-diabetic effects. [25][26][27] However, studies on the effect of PCG on obesity-related pathogenesis and its mechanism are limited. In this study, the effects of PCG on obesity-induced inflammation and oxidative stress, and its mechanism, were investigated in co-culture systems and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Punicalagin, a Pomegranate‐Derived Ellagitannin, Suppresses Obesity and Obesity‐Induced Inflammatory Responses Via the Nrf2/Keap1 Signaling Pathway

Kang

Kim

Hwang

et al. 2019

Molecular Nutrition Food Res

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Scope Punicalagin (PCG) is one of the most abundant phytochemicals found in pomegranates. The effects and mechanistic action of PCG on obesity and obesity‐induced inflammatory and oxidant responses are investigated in vitro and in vivo. Methods and results The effect of PCG on adipogenesis is examined using Oil red O staining. The effects and mechanism of action of PCG on inflammatory responses are determined in adipocyte‐conditioned medium (ACM)‐cultured macrophages, a cell‐to‐cell contact system, and a transwell system. The effects of PCG on obesity and obesity‐induced inflammatory/oxidant responses are examined in high‐fat diet (HFD)‐fed mice. PCG effectively suppresses lipid accumulation in adipocytes and adipocyte‐induced inflammatory responses in adipocyte‐macrophage co‐culture systems. Small interfering RNA (siRNA) transfection indicates that the PCG‐mediated anti‐inflammatory effect is exerted via the nuclear factor erythroid 2‐related factor 2/Kelch‐like ECH‐associated protein 1(Nrf2/Keap1) pathway. PCG administration results in a significant reduction in body and white adipose tissue (WAT) weights. PCG favorably regulates pro‐ and anti‐inflammatory cytokines, downregulating nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB). Immunohistochemical (IHC) analysis demonstrates that PCG differentially modulates the distribution of complement component 3 receptor 4 subunit (CD11c) and cluster of differentiation 206 (CD206). PCG regulates the level of antioxidant and oxidant molecules by activating Nrf2/Keap1 signaling. Conclusions PCG ameliorates obesity and obesity‐induced inflammatory responses via activation of Nrf2/Keap1 signaling, suggesting that PCG has potential as an oral agent to control obesity‐mediated diseases.

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“…In addition, some legumes contain bioactive compounds that can influence glucose metabolism (Ge R tek et al 2014). A large number of these bioactive molecules are being investigated for their mechanism of action and some compounds were found to inhibit JNK gene and protein expression (Bhuvaneswari et al 2014;Jiang et al 2015;Li et al 2015;El-Beih et al 2019;Zhang et al 2019). Further research is needed to understand the inhibition of these molecules by natural compounds.…”

Section: Introductionmentioning

confidence: 99%

Effect of Lupinus rotundiflorus gamma conglutin treatment on JNK1 gene expression and protein activation in a rat model of type 2 diabetes

Zepeda-Peña

Gurrola-Díaz

Domı́nguez-Rosales

et al. 2021

Pharmaceutical Biology

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Context: Gamma conglutin (Cc) from lupine species represents a potential complementary treatment for type 2 diabetes mellitus (T2DM) because of its hypoglycaemic effect. However, its underlying mechanism of action is not fully known. Objective: To evaluate whether Cc from Lupinus rotundiflorus M. E. Jones (Fabaceae) modulates c-Jun Nterminal kinase 1 (JNK1) expression and activation in a T2DM rat model. Materials and methods: Gamma conglutin isolated from L. rotundiflorus seeds was characterized by SDS-PAGE. Fifteen Wistar rats with streptozotocin-induced T2DM (HG) were randomized into three groups (n ¼ 5): vehicle administration (HG-Ctrl), oral treatment with Cc (120 mg/kg/day) (HG-Lr) for one week, and treatment with metformin (300 mg/kg/day) (HG-Met); a healthy group (Ctrl, n ¼ 5) was included as control. The levels of glucose and biomarkers of renal and hepatic function were measured pre-and post-treatment. Hepatic Jnk1 expression and phosphorylation of JNK1 were evaluated by qRT-PCR and western blot, respectively. Results: Oral treatment with either Cc or metformin reduced serum glucose level to 86.30 and 74.80 mg/ dL, respectively (p ˂ 0.05), from the basal levels. Jnk1 expression was 0.65-and 0.54-fold lower (p ˂ 0.05) in the HG-Lr and HG-Met groups, respectively, than in HG-Ctrl. Treatment with Cc decreased JNK1 phosphorylation. However, Cc did not change the levels of kidney and liver biomarkers. Discussion and conclusions: Treatment with Cc from L. rotundiflorus inhibited Jnk1 expression, in vivo, suggesting JNK1 as a potential therapeutic target in diabetes and revealing one mechanism underlying the hypoglycaemic effect of lupine Cc. Nevertheless, further studies are required.

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Effects of pomegranate aril juice and its punicalagin on some key regulators of insulin resistance and oxidative liver injury in streptozotocin-nicotinamide type 2 diabetic rats

Cited by 24 publications

References 41 publications

Evaluation of the in vitro ⍺‐amylase inhibitory, antiglycation, and antioxidant properties ofPunica granatumL. (pomegranate) fruit peel acetone extract and its effect on glucose uptake and oxidative stress in hepatocytes

Evaluation of the in vitro ⍺‐amylase inhibitory, antiglycation, and antioxidant properties ofPunica granatumL. (pomegranate) fruit peel acetone extract and its effect on glucose uptake and oxidative stress in hepatocytes

Punicalagin, a Pomegranate‐Derived Ellagitannin, Suppresses Obesity and Obesity‐Induced Inflammatory Responses Via the Nrf2/Keap1 Signaling Pathway

Effect of Lupinus rotundiflorus gamma conglutin treatment on JNK1 gene expression and protein activation in a rat model of type 2 diabetes

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