Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara

Abstract

The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.

Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots

“…In the present study, culture of jute cotyledons with attached petioles in the presence of either commercial grade or purified Pluronic F-68 up to 0.5% (w/v) increased shoot growth, with no further stimulation at higher concentrations. In contrast, maximum growth of S. dulcamara transformed roots occurred in the presence of 0.01% (w/v) commercial grade Pluronic, and that of leaf-derived callus at 0.1% (Kumar et al 1991(Kumar et al , 1992. Furthermore, cell suspension cultures of S. dulcamara will grow normally in the presence of Pluronic up to 1.0% (w/v), although growth is inhibited at higher concentrations (King et al 1990).…”

“…Previous work has shown that the non-ionic, block co-polymer surfactant, Pluronic F-68 (Poloxamer 188; Fig. 1), is a valuable growth-promoting supplement in transformed root, callus and protoplast cultures of Solanum dulcamara (Kumar et al 1991(Kumar et al , 1992. Although the mechanism(s) of this growth-promoting effect were not determined, related studies with animal cells have shown that Pluronic F-68 stimulates increased 2-deoxyglucose uptake and amino acid incorporation into protein (Cawrse et al 1991), suggesting an enhancement of nutrient uptake from culture medium.…”

Conversion of mouse fibroblasts to sphere cells induced by AlbuMAXI-containing medium

“…It has been used in early postsurgical wound healing to facilitate early attachment and enhance the growth rate of human gingival fibroblasts (17). Pluronic F-68 can also improve plant protoplast proliferation, cell proliferation, bud induction and shoot regeneration (18). Albumin-associated lipids have been shown to play a role in regulating human ES cell self-renewal, but little is known about the role of these lipids in cellular reprogramming (19).…”

Conversion of mouse fibroblasts to sphere cells induced by AlbuMAXI-containing medium

“…It has been used in early postsurgical wound healing to facilitate early attachment and enhance the growth rate of human gingival fibroblasts (17). Pluronic F-68 can also improve plant protoplast proliferation, cell proliferation, bud induction and shoot regeneration (18). Albumin-associated lipids have been shown to play a role in regulating human ES cell self-renewal, but little is known about the role of these lipids in cellular reprogramming (19).…”