Effects of Plasma Rich in Growth Factors on Bone Formation in Rat Calvaria

Abstract

Abstruct: Plasma rich in growth factors (PRGF) had been used in regenerative therapy such as soft tissue and newly bone formation. However, there has been little basic research into the efficacy of PRGF in bone regeneration. In this study, we evaluated the efficacy for new bone formation by transplantation of PRGF onto rat calvaria. The osteogenic potential was evaluated by histologic findings, immunohistochemistry and bone formation analyzed with micro-computed tomography (micro-CT). PRGF was prepared by centrifugation of rat whole blood (WB), and then was activated using 10% calcium chloride solution. The activated PRGF transplanted within a polytetrafluoroethylene (PTFE) tube was transplanted onto calvarial bone of rats. Histological observation demonstrated that PRGF group showed newly formed bone in a wide range. Immunohistochemistry showed Runx2, Osterix, Bone Alkaline Phosphatase (BAP) and Osteocalcin expressed in PRGF group during the early stage of bone formation. Micro-CT showed that PRGF group promoted an increase in bone volume which compared to control group. We concluded that PRGF has more capacity for bone regeneration, and PRGF may be useful in bone regeneration treatment.

Platelet-Rich Plasma in Grafted Maxillae

“…Hatakeyama et al have used calcium chloride for PRP activation, and it was previously reported that calcium BADR ET AL IMPLANT DENTISTRY / VOLUME 25, NUMBER 3 2016 chloride alone might not lead to proper platelet degranulation, 32 which might subsequently affect the outcome. On the other hand, our results were in agreement with Eda et al 33 in their experimental study in which they demonstrated more newly formed bone in the PRP group compared to the control group in rat calvarial defects.…”

Histological Evaluation of Alveolar Bone Ridge for Dental Implant Placement Using a Nondecalcified Frozen Section Technique

“…However, the majority of these have used the decalcified sectioning method 17,18) . To date, reports of studies such as the present, with biological analysis of bone quality at jawbone site, have been non-existent both in Japan and overseas due to 63 This study used the nondecalcified frozen section technique to evaluate the bone quality of harvested bone by site.…”