A high amount of functional membrane protein complex was introduced into a folded-sheet silica mesoporous material (FSM) that has nanometer-size pores of honeycomb-like hexagonal cylindrical structure inside. The photosynthetic light-harvesting complex LH2, which is a typical membrane protein, has a cylindrical structure of 7.3 nm diameter and contains 27 bacteriochlorophyll a and nine carotenoid molecules. The complex captures light energy in the anoxygenic thermophilic purple photosynthetic bacterium Thermochromatium tepidum. The amount of LH2 adsorbed to FSM was determined optically and by the adsorption isotherms of N2. The FSM compounds with internal pore diameters of 7.9 and 2.7 nm adsorbed LH2 at 1.11 and 0.24 mg/mg FSM, respectively, suggesting the high specific affinity of LH2 to the interior of the hydrophobic nanopores with a diameter of 7.9 nm. The LH2 adsorbed to FSM showed almost intact absorption bands of bacteriochlorophylls, and was fully active in the capture and transfer of excitation energy. The LH2 complex inside the FSM showed increased heat stability of the exciton-type absorption band of bacteriochlorophylls (B850), suggesting higher circular symmetry. The environment inside the hydrophobic silica nanopores can be a new matrix for the membrane proteins to reveal their functions. The silica-membrane protein adduct will be useful for the construction of new probes and reaction systems.
The dynamic aspects in latex particle dispersions were studied using video imagery combined with an image data analyzer and Kossel line analysis. The lattice vibrations and lattice defects in the colloidal crystals were demonstrated. The trajectories of particles in the ordered regions were shown to be quite different from those in coexisting disordered regions. The kinetics of (2D) crystal growth was followed by using the microscopic information (as a density function) and its Fourier transformation. The process was also followed by the radial distribution function g(r), which was obtained by direct measurements of interparticle distances. The Ostwald ripening mechanism was confirmed in the process of crystallization.
SUMMARYThis paper presents the results of a pilot test conducted for evaluating the energy dissipation behaviour of shear panels made of low yield steel whose 0-2 per cent offset yield stress is 120 MPa. A total of six full-scale shear panels were tested with the loading condition, stiffener spacing, and magnitude of axial force as test variables. The shear panels tested yielded at a shear force that is approximately 4 of the yield shear force of equivalent shear panels made of common mild steel. Shear panels with proper stiffener arrangement exhibited stable hysteresis, thus ensuring large energy dissipation capacity. Sufficient strain hardening was observed in the shear panels tested, with their energy dissipation capacity about 1.5 times larger than that of an equivalent linear-elastic and perfect-plastic system. Plate buckling did not lead the shear panels to immediate degradation in their energy dissipation capacity. Post-buckling resistance was found to be a subject that requires further studies for quantifying the performance of shear panels made of low yield stress steel as hysteretic dampers.
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