Effects of plant protease inhibitors, oryzacystatin I and soybean Bowman–Birk inhibitor, on the aphid Macrosiphum euphorbiae (Homoptera, Aphididae) and its parasitoid Aphelinus abdominalis (Hymenoptera, Aphelinidae)

Azzouz, Hichem; Cherqui, Anas; Campan, Erick; Rahbé, Yvan; Duport, G.; Jouanin, Lise; Kaiser, Laure; Giordanengo, Philippe

doi:10.1016/j.jinsphys.2004.11.010

Cited by 88 publications

(62 citation statements)

References 46 publications

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“…In 2002, Banks et al have isolated trypsin/chymotrypsin inhibitor with potent antifungal properties from broad bean (Banks et al 2002). In addition, several studies have demonstrated that cysteine and serine proteases, in particular, reduced fecundity, delayed development, and growth and may also increase mortality (Ryan 1990;Oppert et al 1993;Annadana et al 2002;Oppert et al 2003;Azzouz et al 2005). Thus, the protease inhibitor isolated from R. frangula could be developed for use as a possible drug in pharmaceutical industries and also for the direct application as a biocontrol agent for the protection of plants against phytopathogenic fungi.…”

Section: Effect Of the Rfip1 On Proteases With Therapeutic Importancementioning

confidence: 99%

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

et al. 2017

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Protease inhibitors from plants are well known to be potent inhibitors of the growth of bacteria, fungi, and even certain viruses which make them excellent candidates for use as the lead compounds for the development of novel antimicrobial agents for applications in medicine. In this study, Rhamnus frangula was selected as a protease inhibitor source. The maximum recovery of the protease inhibitor against trypsin was recorded in the crude extract made in 0.1 M phosphate buffer (pH 7.0) and isolated from the mature leaves. Then, the protease inhibitor designated as RfIP1 was purified to homogeneity by Sephadex G50 with an apparent molecular mass of 22.5 kDa and its N-terminal sequence exhibited a high degree of homology with known serine protease inhibitor sequences. The RfIP1 displayed maximal activity at pH 7 and 37°C. It maintained almost 80% of its maximal activity through a large pH range. The thermo-stability of RfIP1 was markedly enhanced by BSA, CaCl 2, and sorbitol, whereas the addition of Mg 2? , Zn 2? , NaTDC, SDS, DTT, and b-ME significantly promoted inhibitory activity. The protease inhibitor displayed high inhibitory activity toward some known proteases (cathepsin B, chymotrypsin, collagenase, thrombin, and trypsin) that have more importance in pharmaceutical industry and it acted as potent inhibitor of some commercially proteases from Aspergillus oryzae, Bacillus sp, and Bacillus licheniformis. The protease inhibitor also possessed an appreciable antibacterial effect against both Gram-positive and Gram-negative bacteria.

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Section: Effect Of the Rfip1 On Proteases With Therapeutic Importancementioning

confidence: 99%

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

et al. 2017

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“…lepidopteran, 75 and hemipteran insects. 76 The success of transgenic crops in expressing PIs against insect pests has accentuated the need to understand the mechanisms, and interactions of multiple PIs with other defenses, and the adaptive responses of the herbivores.…”

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confidence: 99%

“…74,75 PIs bind to the digestive enzymes in insect gut and inhibit their activity, thereby reduce protein digestion, resulting in the shortage of amino acids, and slow development and/or starvation of the insects. 76 The defensive function of many PIs against insect pests, directly or by expression in transgenic plants to improve plant resistance against insects has been studied against many litura (Fab. ), H. armigera, Bemisia tabaci (Gen.), Tetranychus cinnabarinus (Boisd.…”

mentioning

confidence: 99%

Mechanisms of plant defense against insect herbivores

War

Paulraj

Ahmad

et al. 2012

Plant Signaling & Behavior

1,553

1,129

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“…The cysteine protease inhibitor oryzacystatin-1 (OC-1), derived from rice seed [108], has been widely demonstrated to exert deleterious effects on a number of economically important insects, including coleopteran [109,110] and homopteran pests [63,111]. Protection against plant-pathogenic nematodes has also been demonstrated [112].…”

Section: Decemlineatamentioning

confidence: 99%

Insect-resistant biotech crops and their impacts on beneficial arthropods

Gatehouse

Ferry

Edwards

et al. 2011

Phil. Trans. R. Soc. B

104

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With a projected population of 10 billion by 2050, an immediate priority for agriculture is to achieve increased crop yields in a sustainable and cost-effective way. The concept of using a transgenic approach was realized in the mid-1990s with the commercial introduction of genetically modified (GM) crops. By 2010, the global value of the seed alone was US $11.2 billion, with commercial biotech maize, soya bean grain and cotton valued at approximately US $150 billion. In recent years, it has become evident that insect-resistant crops expressing δ -endotoxin genes from Bacillus thuringiensis have made a significant beneficial impact on global agriculture, not least in terms of pest reduction and improved quality. However, because of the potential for pest populations to evolve resistance, and owing to lack of effective control of homopteran pests, alternative strategies are being developed. Some of these are based on Bacillus spp. or other insect pathogens, while others are based on the use of plant- and animal-derived genes. However, if such approaches are to play a useful role in crop protection, it is desirable that they do not have a negative impact on beneficial organisms at higher trophic levels thus affecting the functioning of the agro-ecosystem. This widely held concern over the ecological impacts of GM crops has led to the extensive examination of the potential effects of a range of transgene proteins on non-target and beneficial insects. The findings to date with respect to both commercial and experimental GM crops expressing anti-insect genes are discussed here, with particular emphasis on insect predators and parasitoids.

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Effects of plant protease inhibitors, oryzacystatin I and soybean Bowman–Birk inhibitor, on the aphid Macrosiphum euphorbiae (Homoptera, Aphididae) and its parasitoid Aphelinus abdominalis (Hymenoptera, Aphelinidae)

Cited by 88 publications

References 46 publications

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

Purification and characterization of a newly serine protease inhibitor from Rhamnus frangula with potential for use as therapeutic drug

Mechanisms of plant defense against insect herbivores

Insect-resistant biotech crops and their impacts on beneficial arthropods

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