Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects

Abstract

Aim To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP). Materials and Methods Twenty five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analyzed using multiplex bead immunoassay for: GM-CSF, IFN-γ, IL-10, IL-1β, IL-2, IL-6 and TNF-α. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analyzed using the Wilcoxon test and between groups using the Mann-Whitney test. Results Significant reductions in GCF GM-CSF, IL-1β and the ratio IL-1β/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups. Conclusions Periodontal therapy improved GCF cytokine profiles by lowering IL-1β and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines.

IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters

“…It was demonstrated that periodontal therapy improved GCF cytokine profiles by lowering IL-1β and increasing IL-10 levels but the mean change in IL-10 did not differ between AgP and control groups. 29 Similarly, these findings were observed in our previous study. 7 In the literature, there are few studies investigating the relationship between AgP and IL-6 gene polymorphisms.…”

“…We showed that reduced clinical inflammation was related to lower GCF IL-6 cytokine level. Contrary to our study, Oliveira et al 29 reported that GCF IL-6 level was increased in GAgP patients after treatment, but not significantly. The authors explained this situation by affirming that IL-6 acts both as pro-and anti-inflammatory cytokine at the same time.…”

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

“…They can also be used to discriminate healthy subjects from patients with AgP [41, 51, 80, 99]. Owing to the slight differences in IL-1 β levels between healthy and gingivitis sites [41, 51, 103], it is difficult to use them as indicators or predictors for disease initiation from healthy status to gingivitis.…”

Effects of periodontal treatment on primary sjȫgren’s syndrome symptoms

“…Sterile paper strips (Periopaper Strip ® Collection, Oraflow, Plainview, NY, USA) were then inserted for 30 seconds. 20 Samples visibly contaminated with blood were discarded. The volume Lost to follow-up: n = 0 n = 7 with complete data n = 7 analyzed…”