Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants

Petri, Maximilian; Ufer, K.; Toma, Ionel; Becher, Christoph; Liodakis, Emmanouil; Brand, Stephan; Haas, Philipp; Liu, C.; Richter, B.; Haasper, Carl; Lewinski, Gabriela von; Jagodzinski, Michael

doi:10.1007/s00167-011-1600-3

Cited by 48 publications

(28 citation statements)

References 44 publications

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“…Menaflex is derived from devitalized bovine collagen (Achilles tendon) and this scaffold allows cellular ingrowth and improves tissue regeneration [10,12]. The use of Bone marrow derived stem cells under perfusion and cyclic compression has been shown to have a positive effect on the cellular ingrowth in vitro [13]. So far, several studies have proven the positive effect of Menaflex implantation on the clinical outcome measured by IKDC, Tegner Score and VAS [14-16].…”

Section: Introductionmentioning

confidence: 99%

Biomechanical comparison of menisci from different species and artificial constructs

Sandmann

Adamczyk

García

et al. 2013

BMC Musculoskelet Disord

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BackgroundLoss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. The purpose of the current study was to biomechanically characterize bovine, ovine, and porcine menisci (each n = 6, midpart of the medial meniscus) and compare their properties to that of normal and degenerated human menisci (n = 6) and two commercially available artificial scaffolds (each n = 3).MethodsSamples were tested in a cyclic, minimally constraint compression–relaxation test with a universal testing machine allowing the characterization of the viscoelastic properties including stiffness, residual force and relative sample compression. T-tests were used to compare the biomechanical parameters of all samples. Significance level was set at p < 0.05.ResultsThroughout cyclic testing stiffness, residual force and relative sample compression increased significantly (p < 0.05) in all tested meniscus samples. From the tested animal meniscus samples the ovine menisci showed the highest biomechanical similarity to human menisci in terms of stiffness (human: 8.54 N/mm ± 1.87, cycle 1; ovine: 11.24 N/mm ± 2.36, cycle 1, p = 0.0528), residual force (human: 2.99 N ± 0.63, cycle 1 vs. ovine 3.24 N ± 0.13, cycle 1, p = 0.364) and relative sample compression (human 19.92% ± 0.63, cycle 1 vs. 18.72% ± 1.84 in ovine samples at cycle 1, p = 0.162). The artificial constructs -as hypothesized- revealed statistically significant inferior biomechanical properties.ConclusionsFor future research the use of ovine meniscus would be desirable showing the highest biomechanical similarities to human meniscus tissue. The significantly different biomechanical properties of the artificial scaffolds highlight the necessity of cellular ingrowth and formation of extracellular matrix to gain viscoelastic properties. As a consequence, a period of unloading (at least partial weight bearing) is necessary, until the remodeling process in the scaffold is sufficient to withstand forces during weight bearing.

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Section: Introductionmentioning

confidence: 99%

Biomechanical comparison of menisci from different species and artificial constructs

Sandmann

Adamczyk

García

et al. 2013

BMC Musculoskelet Disord

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show abstract

“…Dynamic compression on micro-channeled scaffolds resulted in aligned cell layers and collagen fibers [96] and hydrostatic pressure combined with growth factor can enhance compressive properties [97]. Other stimulations were also reported to increase ECM production and newly formed tissues’ mechanical properties, such as tension-compression loading [98], perfusion and cyclic compression [99]. …”

Section: Scaffold Propertiesmentioning

confidence: 99%

An Overview of Scaffold Design and Fabrication Technology for Engineered Knee Meniscus

2017

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Current surgical treatments for meniscal tears suffer from subsequent degeneration of knee joints, limited donor organs and inconsistent post-treatment results. Three clinical scaffolds (Menaflex CMI, Actifit ® scaffold and NUsurface ® Meniscus Implant) are available on the market, but additional data are needed to properly evaluate their safety and effectiveness. Thus, many scaffold-based research activities have been done to develop new materials, structures and fabrication technologies to mimic native meniscus for cell attachment and subsequent tissue development, and restore functionalities of injured meniscus for long-term effects. This study begins with a synopsis of relevant structural features of meniscus and goes on to describe the critical considerations. Promising advances made in the field of meniscal scaffolding technology, in terms of biocompatible materials, fabrication methods, structure design and their impact on mechanical and biological properties are discussed in detail. Among all the scaffolding technologies, additive manufacturing (AM) is very promising because of its ability to precisely control fiber diameter, orientation, and pore network micro-architecture to mimic the native meniscus microenvironment.

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“…Similar results were shown in a study with a collagen scaffold for a meniscus implant. Continuous fluid perfusion in combination with mechanical stimulation improved the biomechanical properties of the in vitro tissue-engineered construct (Petri et al, 2012). A porous poly urethane scaffold intended for a meniscus implant also showed the beneficial effect of fluid perfusion and mechanical stimulation on the biomechanical properties of the tissue-engineered construct (Liu et al, 2012).…”

Section: Mechanical Stimuli and Tissue Formationmentioning

confidence: 99%

The Use of Finite Element Analyses to Design and Fabricate Three-Dimensional Scaffolds for Skeletal Tissue Engineering

Hendrikson

Blitterswijk

Rouwkema

et al. 2017

Front. Bioeng. Biotechnol.

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Computational modeling has been increasingly applied to the field of tissue engineering and regenerative medicine. Where in early days computational models were used to better understand the biomechanical requirements of targeted tissues to be regenerated, recently, more and more models are formulated to combine such biomechanical requirements with cell fate predictions to aid in the design of functional three-dimensional scaffolds. In this review, we highlight how computational modeling has been used to understand the mechanisms behind tissue formation and can be used for more rational and biomimetic scaffold-based tissue regeneration strategies. With a particular focus on musculoskeletal tissues, we discuss recent models attempting to predict cell activity in relation to specific mechanical and physical stimuli that can be applied to them through porous three-dimensional scaffolds. In doing so, we review the most common scaffold fabrication methods, with a critical view on those technologies that offer better properties to be more easily combined with computational modeling. Finally, we discuss how modeling, and in particular finite element analysis, can be used to optimize the design of scaffolds for skeletal tissue regeneration.

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Effects of perfusion and cyclic compression on in vitro tissue engineered meniscus implants

Abstract: Biomechanical stimulation and perfusion have impact on collagen scaffolds seeded with BMSCs. Cell proliferation can be enhanced using continuous perfusion and differentiation is fostered by mechanical stimulation.

Cited by 48 publications

References 44 publications

Biomechanical comparison of menisci from different species and artificial constructs

Biomechanical comparison of menisci from different species and artificial constructs

An Overview of Scaffold Design and Fabrication Technology for Engineered Knee Meniscus

The Use of Finite Element Analyses to Design and Fabricate Three-Dimensional Scaffolds for Skeletal Tissue Engineering

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