Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae

Qin, Chuanjie; De, Zhao; Gong, Quan; Qi, Zemin; Zou, Youlan; Yue, Xingjian; Xie, Bin

doi:10.1016/j.fsi.2013.07.007

Cited by 20 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the fish were exposed to stress, over-expression of HSP70 and HSP90 may have been immediately induced to help the cells relieve stress and adapt to the external environmental changes (Ceyhun et al, 2010). This result was consistent with those of the ammonia toxicity test on Takifugu obscurus (Cheng et al, 2015) and Botia reevesae (Qin et al, 2013). To understand the functions of HSPs, the gene sequences of many marine organisms have been cloned and detected, including the rainbow trout (Ojima et al, 2005), Chinese mitten crab (Li et al, 2009a), black tiger shrimp (Rungrassamee et al, 2010), and silver sea bream (Deane and Woo, 2005).…”

Section: Discussionsupporting

confidence: 90%

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

Cao

Wang

et al. 2018

Preprint

View full text Add to dashboard Cite

2The silver pomfret (Pampus argenteus) is a major economically important marine fish in China. 3However, P. argenteus is sensitive to many stress factors and susceptible to injury. This problem could 2 4 be resolved using anesthesia. We determined the lowest effective dose (LED) of tricaine 2 5 methanesulfonate (MS-222) and assessed the longest safe deep anesthesia time and effect after 2 6 aquaculture treatment stresses. P. argenteus juveniles were exposed to six concentrations of MS-222 2 7(10, 25, 50, 75, 100, and 125 mg L -1 ); LED was established at 75 mg L -1 . The juveniles were exposed 2 8to different deep anesthesia times (4, 7, 10, 12, and 15 min) at 75 mg L -1 ; the longest safe deep 2 9anesthesia time under LED was 10 min. Finally, the juveniles were randomly divided into four groups: 3 0 control group (CG), draining group (DG, drain), anesthetic group (AG, drain + MS-222 + aquaculture 3 1 treatment); and non-anesthetic group (NAG, drain + aquaculture treatment). Plasma cortisol levels in 3 2 the NAG, AG, DG, and CG groups were 38.739 ± 1.065 (highest), 25.083 ± 0.587, 28.644 ± 0.612, and 3 322.620 ± 0.836 ng mL -1 (lowest). The AG group showed significant differences in superoxide 3 4 dismutase, catalase, and malondialdehyde activities, except for glutathione. HSP70, HSP90, GR1, and 3 5GR2 mRNA levels in the NAG group increased sharply in response to stressors. GR1 and GR2 mRNA 3 6 levels in the AG group also increased significantly, whereas HSP70 and HSP90 mRNA levels showed 3 7 no significant differences. Thus, MS-222 can reduce oxidative damage, stress reaction, and resistance 3 8to aquaculture treatment stresses in P. argenteus.

show abstract

Section: Discussionsupporting

confidence: 90%

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

Cao

Wang

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…A large amount of study has reported that the expression pattern of Hsp70 gene can be induced by various stress factors ( Arai et al, 1995 ; Liu et al, 2014 ). They play an important role, not only in protein folding, membrane, translocated, misfolded, protein degradation and other cell-protecting processes, but also responses to stress, bacterial infection, parasitism and inflammatory ( Qin et al, 2013 ). Members of the Hsp70 gene family has been systematically described in a number of species, such as Humans, birds, amphibians, zebrafish, catfish, and medaka ( Song et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Luo

2020

PeerJ

View full text Add to dashboard Cite

Heat shock proteins (Hsps) play an important role in many biological processes. However, as a typical cold water fish, the systematic identification of Hsp70/110 gene family of rainbow trout (Oncorhynchus mykiss) has not been reported, and the role of Hsp70/110 gene in the evolution of rainbow trout has not been described systematically. In this study, bioinformatics methods were used to analyze the Hsp70/110 gene family of rainbow trout. A total of 16 hsp70/110 genes were identified and classified into ten subgroups. The 16 Hsp70/110 genes were all distributed on chromosomes 2, 4, 8 and 13. The molecular weight is ranged from 78.93 to 91.39 kD. Gene structure and motif composition are relatively conserved in each subgroup. According to RNA-seq analysis of rainbow trout liver and head kidney, a total of four out of 16 genes were significantly upregulated in liver under heat stress, and a total of seven out of 16 genes were significantly upregulated in head kidney. RT-qPCR was carried out on these gene, and the result were consistent with those of RNA-seq. The significantly regulated expressions of Hsp70/110 genes under heat stress indicats that Hsp70/110 genes are involved in heat stress response in rainbow trout. This systematic analysis provided valuable information about the diverse roles of Hsp70/110 in the evolution of teleost, which will contribute to the functional characterization of Hsp70/110 genes in further research.

show abstract

“…Qin et al (2013) reported that the level of HSP-70 exp ression in tissues regulates the biological processes in fish that reduce the deleterious effects of environmental stress. Zhang and Zhang (2012) have shown that Cu 2+ and malachite green significantly up regulate hemocyte HSP-70 mRNA expression in the digestive glands and gills of oysters (Crassostrea hongkongensis).…”

Section: Discussionmentioning

confidence: 99%

“…GSTs are involved in the detoxication of many chemical compounds including heavy metal, hydrocarbons, organochlorine insecticides and polychlorinated biphenyls (PCBs) (George and Buchanan, 1990). Heat shock proteins (HSP) are a subset of molecu lar chaperones, and play key role in the process of protein metabolism under stress conditions, including the refolding of denatured protein, maintenance of structure integrity and other regulatory processes (Feder and Ho fmann, 1999 needed even more after stress such heavy metals (Qin et al, 2013). HSP-70 proteins are highly conserved across all liv ing organisms, not only in de novo protein folding, membrane translocation, degradation of misfolded proteins and other p rocesses for the protection of cells, but also in responses to stress and inflammation (Jing et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Jing¹,

Li²,

Zhang³

et al. 2017

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

A study was carried to test the response of mudskipper for 8 days under four lead concentrations. Fish exposed to 2 mg/L Pb had the lowest glutathione peroxidase (GPX) in liver and glutathione (G SH) activities in liver and gill. Liver superoxide dismutase (SOD) in fish exposed to 1 and 2 mg/L Pb was lower than that in fish exposed to 0 and 0.5 mg/L Pb. However, the higher content of malondialdehyde (M DA) in gill and liver was found in 2 mg/L Pb group. The lower SOD, glutathione s-transferase (G ST), heat shock proteins 70 (HSP-70) and HSP-90 expression in gill and liver were found in 0 mg/L Pb group. During the 8-day period, catalase(CAT), GPX and GSH activates in gill were the lowest at 8d. The lowest CAT activity in liver and the highest M DA content in gill and liver were found at day 8. The highest values of SOD and HSP -90 expression in gill were found at day 2. but the highest values of HSP-70 expression in liver were found from days 6 to 8. This study indicated that Pb-induced ROS generation is not fully counteracted by antioxidant enzymes. Functional enzyme activity was affected by mRNA expression.

show abstract

Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae

Cited by 20 publications

References 30 publications

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

Effects of the anesthetic MS-222 on silver pomfret (Pampus argenteus) juveniles under aquaculture treatment stresses

Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

Untitled

Contact Info

Product

Resources

About