Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar toothIn vitro

Sakakura, Yasunori

doi:10.1007/bf02555728

Cited by 14 publications

(11 citation statements)

References 19 publications

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“…Moreover, the Tm7 region shows significant homology to the glucagon/vasoactive intestinal peptide/calcitonin receptor family that includes the receptors for calcitonin (CT), parathyroid hormone (PTH), and parathyroid hormonerelated peptide (PTHrP), among others . CT and PTH each have an influence on the differentiation of odontoblasts and the formation of predentin and dentin (Sakakura 1987;Sakakura et al 1984Sakakura et al , 1989, and significantly increase the intracellular cyclic AMP levels of isolated dental papilla cells (Shteyer et al 1987) and active odontoblasts expressing PTH receptors (Lundgren et al 1998). In addition, PTHrP and its receptor mRNAs are expressed in dental epithelium and dental papilla, respectively (Beck et al 1995;Lee et al 1995;Liu et al 1998).…”

Section: Discussionmentioning

confidence: 98%

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Tsuruga

Sakakura

Yajima

et al. 1999

Histochemistry and Cell Biology

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Dendritic cells and macrophages were examined in dental pulp during the postnatal development of mouse mandibular first molars, by immuno- and enzyme histochemistry. F4/80 antibody against dendritic cells and macrophages demonstrated labeled cells predominantly in and around the odontoblastic layer during tooth development from postnatal day 0 (PN0) to PN5. Labeling with Mac-1, Mac-2, and MOMA-2 antibodies against macrophages showed varied distribution patterns. Mac-1-positive cells were not detected in the dental pulp. Mac-2-positive cells appeared in the dental pulp at PN0, but not in or around the odontoblastic layer, and disappeared by PN3. A few MOMA-2-positive cells were detected in the dental pulp during the period examined. The F4/80-positive cells in and around the odontoblastic layer did not exhibit acid phosphatase or non-specific esterase activities. In addition, the F4/80-positive cells showed continued expression of Fcgamma receptor, but not class II major histocompatibility complex (MHC). Other antibodies against dendritic cells (NLDC-145, MIDC-8, and 33D1) did not label the F4/80-positive cells. We concluded that the F4/80-positive and class II MHC-negative cells in and around the odontoblastic layer may be immature dendritic cells in the early stages before eruption, weaning, and crucial exposure to antigenic stimuli. They may not only act primarily as immunosurveillance cells, but also play a role in a regulatory function and differentiation of odontoblasts.

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Section: Discussionmentioning

confidence: 98%

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Tsuruga

Sakakura

Yajima

et al. 1999

Histochemistry and Cell Biology

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“…However, in vtb studies of the effects of calcium-regulating hormones (PTHH, CT, and vitariin Di 3 on odontoigenesis-in eluding the mineralization of dentin and enamel-have been reported only by our laboratory (Sakakura ci at, 1984(Sakakura ci at, , 1988Sakakura, 1987).…”

Section: Methodsmentioning

confidence: 94%

“…A previous report showed the drastic effect of PT1H on odontogenesis in Wmar (Sakakura, 1987). PTH inhibited the cyto differentiation of pre-odontoblasts into odontoblasts.…”

Section: Methodsmentioning

confidence: 95%

“…-Eighty-nine mandibular first molar tooth germs from 17-day-old ddY mouse embryos were removed aseptically. The molars represented the cap stage of tooth morphogenesis, and both inner enamel epithelium and dental papilla cells remained undifferentiated (Sakakura, 1987). The culture medium consisted of minimum essential medium (alpha modification, GIBCO, Grand Island, New York) supplemented with 10% fetal bovine serum (Flow Lab., North Ryde, Australia) and 60 mg/L kanamycin.…”

Section: Methodsmentioning

confidence: 99%

“…A partial explanation of such inconsistent reports was provided by Mallek et al (1979), who indicated that the influence of CT on calcium uptake in vitro varies with the developmental stages of tooth germs, as well as with the kind of tooth germ, i.e., incisor or molar. Sakakura et al (1984) and Sakakura (1987) studied the effects of CT and PTH on the odontogenesis of mouse embryonic molars in vitro. They reported that two concentrations of CT had different effects on dentinogenesis, either suppressing or promoting the differentiation of odontoblasts and predentin formation.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Effects of Calcitonin and/or Parathyroid Hormone on Odontogenesis of Mouse Embryonic Molars

et al. 1989

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Mandibular first molars of mouse embryos were cultivated for examination of the effects of calcitonin (CT) and/or parathyroid hormone (PTH) on the odontogenesis of the molars, and for determination of whether and how CT, which is a PTH antagonist, has an influence on the effect of PTH on odontogenesis. On the second day, the inner enamel epithelium in the control group had already differentiated into pre-ameloblasts. Typical odontoblasts had secreted a layer of predentin. On the fourth day of culture, the pre-ameloblasts achieved terminal differentiation into secretory ameloblasts, and enamel and dentin had already been deposited. PTH (1 unit/mL) inhibited the odontogenesis of the cultured molars during the designated culture periods (two and four days), while CT (0.5 unit/mL) stimulated odontogenesis. On the second day, the development of the molars in the CT + PTH group showed an intermediate stage between the control and PTH-treated explants, but on day 4 it corresponded to that of the controls. Moreover, when the molars exposed to PTH for two days were untreated and treated with CT for an additional two days, the former produced a small quantity of enamel matrix, while the latter formed a large amount of the matrix. These histological findings were also supported by a morphometric analysis of the enamel matrix in the cultured molars. The present results suggest that CT stimulates, but PTH suppresses, the odontogenesis of the mouse embryonic molars, and that CT is an antagonist to the inhibitory effect of PTH on odontogenesis.

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Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

et al. 1988

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Mandibular first molars from 17-day-old mouse embryos were cultured for 2 and 4 days in alpha-MEM supplemented with 10% newborn bovine serum containing 0.02% ethanol or 0.1, 1.0 or 10 ng/ml 1,25-(OH)2D3. After embedding, every 6th section was stained and mitotic features of inner dental epithelium (IDE) and dental papilla cells (DP) were counted under a light microscope. On the 2nd day, no significant differences were observed in the IDE and DP mitotic indexes among the control, ethanol, and the three vitamin D groups. On the 4th day, the indexes for all groups decreased. But the IDE indexes for all vitamin D groups were significantly different from those for the control (P less than 0.01) and ethanol (P less than 0.05) groups, whereas the DP index was significant (P less than 0.05) only in the 10 ng/ml vitamin D group. The present results suggest that vitamin D3 may have an influence on cell proliferation of IDE and DP in mouse embryonic molars in vitro.

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Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar toothIn vitro

Cited by 14 publications

References 19 publications

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

In vitro Effects of Calcitonin and/or Parathyroid Hormone on Odontogenesis of Mouse Embryonic Molars

Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

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