Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

Sakakura, Yasunori; Fujiwara, Naoki; Ishizeki, Kiyoto; Nawa, Tokio

doi:10.1007/bf02555168

Cited by 5 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A distinct up‐regulation characterized the last step of ameloblast differentiation when mitosis arrest occurs. In vitro studies have suggested that 1,25(OH) 2 D 3 affects cell proliferation in tooth germ (46) . Therefore, both nuclear‐ and membrane‐initiated pathways may participate to the vitamin D‐dependent epithelial‐mesenchymal cascade leading to proper dental morphogenesis and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Mesbah

Nemere

Papagerakis

et al. 2002

J of Bone & Mineral Res

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Mesbah

Nemere

Papagerakis

et al. 2002

J of Bone & Mineral Res

View full text Add to dashboard Cite

“…Supportive evidence for vitamin D sites of action in teeth was provided from immunohistochemical studies with antibodies to vitamin D receptor protein that was localized in precursor cells, differentiated ameloblasts, and odontoblasts, together with calbindin-D 28K antibodies in odontoblasts (17). Incubation of mouse embryonic molars with 1,25(OHh vitamin D 3 resulted in an increase of the mitotic index of inner dental epithelium, indicating effects on cell proliferation (18). Similarly, in vitamin D-deficient rats, in postnatal molar tooth germ development, vitamin D treatment enhanced cytodifferentiation of pulp cells as well as enamel and dentin mineralization (19).…”

Section: Oral Regionmentioning

confidence: 99%

Vitamin D and the digestive system

Stumpf

2008

Eur. J. Drug Metabol. Pharmacokinet.

View full text Add to dashboard Cite

Target tissues of in vivo receptor binding and deposition of 1,25(OH)2 vitamin D3 and its oxygen analog OCT are reviewed in rats, mice, hamsters and zebra finch, identified with high-resolution microscopic autoradiography. Throughout the digestive system numerous sites with nuclear receptor binding of 3H-1,25(OH)2 vitamin D3 and 3H-OCT exist: in the oral region, epithelial cells of the oral cavity, tongue and gingiva, teeth odontoblast and ameloblast precursor pulp and stratum intermedium cells; in the parotid, submandibular and sublingual salivary glands, epithelial cells of striated ducts and granular convoluted tubules, intercalated ducts and acinar cells, as well as myoepithelial cells; in the stomach, neck mucous cells of gastric glands, endocrine cells of the antrum, and muscle cells of the pyloric sphincter; in the small and large intestine, absorptive and crypt epithelial cells; in the pancreas, predominantly islet B-cells. Perisinusoidal stellate (Ito) cells in the liver concentrate and retain variable amounts of radiolabeled compound in regions of their cytoplasm after administration of 3H-I,25(OH)2 vitamin D3 and 3H-25(OH) vitamin D3, probably sites of specific storage, similar to vitamin A. Submucosa in stomach and intestine also retain variable amounts of radiolabel, however unspecific with all compounds studied. In pilot studies with 3H-25(OH)2 vitamin D3 and 3H-24,25(OH)2 vitamin D3, no nuclear concentration was detectable. The reviewed data for vitamin D and its oxygen analogue OCT indicate genomic effects on multiple target tissues of the digestive system that involve cell proliferation and differentiation, endo- and exocrine secretion, digestion and absorption for maintaining optimal functions, with potentials for health prophylaxis and therapies.

show abstract

A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

Sakakura

Fujiwara

Nawa

1989

In Vitro Cell Dev Biol

View full text Add to dashboard Cite

Mandibular first molars from 17-d-old mouse embryos were cultured in vitro for 2 to 4 d by a simple, disposable, improved floatation method. This method consisted of using a 24-well multidish and a plastic culture chamber with a membrane filter. The improved floatation method, as well as our previous method, was capable of the three-dimensional development of tooth germs. Cytodifferentiation of odontoblasts and ameloblasts and formation of extracellular matrices were accelerated by the present culture system, in comparison with our previous method. All the molars cultivated by this method were very similar in morphology to in vivo. On Day 2 of culture the terminal cytodifferentiation of odontoblasts and the formation of predentin were ascertained in the bucco-lingual sections of the cultured molars. A thick layer of predentin was formed at the tip of the cusp and gradually decreased toward the cervical loop and the fissure between the buccal and lingual cusps. On Day 4 in vitro, secretory ameloblasts produced enamel matrix, and the mineralized enamel showed showed prismatic structure very similar to that in vivo. Dentin and predentin also were normal in ultrastructure. The extracellular matrices (enamel, dentine, and predentin) were formed in line with the pattern of the cusp and the formation of matrices normally started at the tip of the cusp. We conclude that the three-dimensional development of whole tooth germs in vitro may be very important for normal expression of the developmental program intrinsic to mouse embryonic molars.

show abstract

Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

Cited by 5 publications

References 14 publications

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Vitamin D and the digestive system

A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

Contact Info

Product

Resources

About

Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

Cited by 5 publications

References 14 publications

Expression of a 1,25‐Dihydroxyvitamin D3 Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Expression of a 1,25‐Dihydroxyvitamin D3 Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Vitamin D and the digestive system

A simple, disposable, and improved organ culture system for maintaining three-dimensional development of mouse embryonic molars

Contact Info

Product

Resources

About

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization

Expression of a 1,25‐Dihydroxyvitamin D₃ Membrane‐Associated Rapid‐Response Steroid Binding Protein During Human Tooth and Bone Development and Biomineralization