Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Tsuruga, Eichi; Sakakura, Yasunori; Yajima, Toshihiko; Shide, Noriyuki

doi:10.1007/s004180050407

Cited by 16 publications

(10 citation statements)

References 66 publications

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“…spleen, intestine, skin, and oral mucosa (Jontell et al, 1991). Further, antigenic characteristics of alacytes are different from those of macrophages and dendritic cells (Tsuruga et al, 1999). The basis for these differences is not completely understood.…”

Section: Telacytes: Astrocyte-like Glial Cellsmentioning

confidence: 98%

Blueprint of an ancestral neurosensory organ revealed in glial networks in human dental pulp

Farahani

Simonian

Hunter

2011

J of Comparative Neurology

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Sensory function of human dental pulp has long been known. A composite role has been suggested for odontoblasts as sensory cells in addition to the synthesis of dentinal matrix. However, the neural basis for such a composite sensory activity remains enigmatic. Here, we aimed to probe the question by pursuing an evolutionary logic; if dental pulp is a vestigial sensory organ co-opted to a function of synthesis of mineralized matrix, essential elements of neurosensory organs may persist in dental pulp. Through structural analysis by confocal laser scanning microscopy, three distinct cell populations adjacent to odontoblasts, glial fibrillary acidic protein (GFAP)(+) seracytes, S100(+) telacytes, and HLA-II(+) alacytes were identified in peripheral human dental pulp. Subsequent molecular fingerprinting by quantitative reverse transcriptase-polymerase chain reaction established these cells as analogous to radial glia (GFAP(+) cells), astrocytes (S100(+) cells), and microglia (HLA-II(+) cells) of central nervous system organs. In the cell-rich zone of the pulp, S100(+) cells formed a network, ensheathed unmyelinated axons, and extended end-feet around the capillaries. The microcirculation adjacent to the glial cells in the cell-rich zone possessed ultrastructural features and a gene expression profile typical of the blood-brain barrier system. These novel findings support a new paradigm for understanding sensory functionality of dental pulp by the demonstration of a sophisticated neural structure in the human dental pulp that is analogous to other central sensory organs. Further, the structure that is revealed informs the concept of the evolutionary origin of the dental pulp, suggesting that a neurosensory organ was the precursor structure of teeth.

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Section: Telacytes: Astrocyte-like Glial Cellsmentioning

confidence: 98%

Blueprint of an ancestral neurosensory organ revealed in glial networks in human dental pulp

Farahani

Simonian

Hunter

2011

J of Comparative Neurology

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“…Immunohistochemistry for mouse macrophages, monocytes, and dendritic cells was performed using the rat anti-mouse F4/80 antibody (Serotec) as described by Tsuruga et al 26 A biotinylated rabbit anti-rat IgG was used as the secondary antibody. Immunoreactivity was visualized by the avidin-biotin peroxidase complex method (Vectastain ABC kit; Vector Laboratories, Burlingame, CA).…”

Section: Zymography and Immunohistochemistrymentioning

confidence: 99%

Tumorigenic Potential of Extracellular Matrix Metalloproteinase Inducer

Zucker

Hymowitz

Rollo

et al. 2001

The American Journal of Pathology

233

202

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Extracellular matrix metalloproteinase inducer (EMMPRIN), a glycoprotein present on the cancer cell plasma membrane, enhances fibroblast synthesis of matrix metalloproteinases (MMPs). The demonstration that peritumoral fibroblasts synthesize most of the MMPs in human tumors rather than the cancer cells themselves has ignited interest in the role of EMMPRIN in tumor dissemination. In this report we have demonstrated a role for EMMPRIN in cancer progression. Human MDA-MB-436 breast cancer cells, which are tumorigenic but slow growing in vivo, were transfected with EMMPRIN cDNA and injected orthotopically into mammary tissue of female NCr nu/nu mice. Green fluorescent protein was used to visualize metastases. In three experiments, breast cancer cell clones transfected with EMMPRIN cDNA were considerably more tumorigenic and invasive than plasmid-transfected cancer cells. Increased gelatinase A and gelatinase B expression (demonstrated by in situ hybridization and gelatin substrate zymography) was demonstrated in EMMPRIN-enhanced tumors. In contrast to de novo breast cancers in humans, human tumors transplanted into mice elicited minimal stromal or inflammatory cell reactions. Based on these experimental studies and our previous demonstration that EMMPRIN is prominently displayed in human cancer tissue, we propose that EMMPRIN plays an important role in cancer progression by increasing synthesis of MMPs.

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“…Several studies have mentioned the possibility of resident macrophages being involved in the regulatory function and differentiation of odontoblasts (Ohshima et al 1995; Tsuruga et al 1999; Nakakura-Ohshima et al 2003). However, the precise functions of the macrophages in the dental pulp have not yet been clarified.…”

Section: Discussionmentioning

confidence: 99%

In situ proliferation and differentiation of macrophages in dental pulp

et al. 2011

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The presence of macrophages in dental pulp is well known. However, whether these macrophages proliferate and differentiate in the dental pulp in situ, or whether they constantly migrate from the blood stream into the dental pulp remains unknown. We have examined and compared the development of dental pulp macrophages in an organ culture system with in vivo tooth organs to clarify the developmental mechanism of these macrophages. The first mandibular molar tooth organs from ICR mice aged between 16 days of gestation (E16) to 5 days postnatally were used for in vivo experiments. Those from E16 were cultured for up to 14 days with or without 10% fetal bovine serum. Dental pulp tissues were analyzed with immunohistochemistry to detect the macrophages and with reverse transcription and the polymerase chain reaction (RT-PCR) for the detection of factors related to macrophage development. The growth curves for the in vivo and in vitro cultured cells revealed similar numbers of F4/80-positive macrophages in the dental pulp. RT-PCR analysis indicated the constant expression of myeloid colony-stimulating factor (M-CSF) in both in-vivo- and in-vitro-cultured dental pulp tissues. Anti-M-CSF antibodies significantly inhibited the increase in the number of macrophages in the dental pulp. These results suggest that (1) most of the dental pulp macrophages proliferate and differentiate in the dental pulp without a supply of precursor cells from the blood stream, (2) M-CSF might be a candidate molecule for dental pulp macrophage development, and (3) serum factors might not directly affect the development of macrophages.

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Appearance and distribution of dendritic cells and macrophages in dental pulp during early postnatal morphogenesis of mouse mandibular first molars

Cited by 16 publications

References 66 publications

Blueprint of an ancestral neurosensory organ revealed in glial networks in human dental pulp

Blueprint of an ancestral neurosensory organ revealed in glial networks in human dental pulp

Tumorigenic Potential of Extracellular Matrix Metalloproteinase Inducer

In situ proliferation and differentiation of macrophages in dental pulp

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