Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model

Abstract

Background/aim: To investigate the effect of ozone and/or taurine treatment comparatively on testicular damage due to ischemia/ reperfusion (I/R) injury in an experimental torsion model in rats.Materials and methods: Adult Wistar rats with and without torsion/detorsion were used. In order to monitor the effect of ozone and/ or taurine treatment on testicular damage due to I/R injury, following histopathological investigation apoptotic indexes were scored by TUNEL method. Moreover, tumor necrosis factor receptor 1 (TNFR1), caspase 3, caspase 8, endothelial nitric oxide synthase (eNOS), tumor necrosis factor alpha (TNFα), and cytochrome C immunostainings were performed and the levels of malondialdehyde, glutathione peroxidase, superoxide dismutase, catalase, total sulfhydryl, and nitric oxide were determined in the testicular tissue.Results: Intraperitoneal ozone and/or taurine treatment prevented both histopathological damage and increase in the apoptotic index. Torsion did not exert an effect on the levels of TNFα and cytochrome C. Ozone and/or taurine treatment prevented increases in TNFR1, caspase 3, and caspase 8. The level of oxidative stress markers was unchanged. The increases in NO level and eNOS expression were prevented by ozone and/or taurine treatment in I/R groups. Conclusion:Using ozone therapy and/or taurine before reperfusion may be a solution for germ cell degeneration resulting from testicular torsion and related infertility.

The protective effect of taurine and/or vanillin against renal, testicular, and hematological alterations induced by potassium bromate toxicity in rats

“…The author reported that exposure to KBrO 3 alters the histology of the rat testis and impaired spermatogenesis. The antioxidant effect of taurine and its protective effect against apoptosis and germ cell degeneration resulting from testicular torsion was reported by Aydos, Başar, Kul, Atmaca, Uzunalioğlu, Kisa, & Efe, 2014).…”

Testicular torsion–detorsion and potential therapeutic treatments: A possible role for ischemic postconditioning

“…Taurine is an organic acid present in mammalian tissues with proven anti‐oxidant properties, and has been used previously in testicular I‐R models . It was found to have a beneficial effect on the ischemic testis; not only could it significantly decrease histopathological damage, apoptosis and MDA, as well as markers for neutrophil, MPO levels, but also could reverse the I‐R injury‐induced damage to spermatogenesis …”

“…39 Taurine is an organic acid present in mammalian tissues with proven anti-oxidant properties, and has been used previously in testicular I-R models. 20,[40][41][42] It was found to have a beneficial effect on the ischemic testis; not only could it significantly decrease histopathological damage, apoptosis and MDA, as well as markers for neutrophil, MPO levels, but also could reverse the I-R injury-induced damage to spermatogenesis. 20,[40][41][42] Previously, Tamamura et al developed a short-term rat model of testicular I-R. 11 The animals were exposed to unilateral torsion for 30-min followed by 1-h of reperfusion.…”

“…20,[40][41][42] It was found to have a beneficial effect on the ischemic testis; not only could it significantly decrease histopathological damage, apoptosis and MDA, as well as markers for neutrophil, MPO levels, but also could reverse the I-R injury-induced damage to spermatogenesis. 20,[40][41][42] Previously, Tamamura et al developed a short-term rat model of testicular I-R. 11 The animals were exposed to unilateral torsion for 30-min followed by 1-h of reperfusion. A high dose of the free radical scavenger, edaravone, significantly reduced MPO levels and HSP-70 protein activity, or partially decreased NO 2 -NO 3 levels, MDA and 8-hydroxy-2 0deoxyguanosine (a marker of oxidative stress to DNA), and further reduced histological alterations, such as vacuolation and necrosis.…”

The effect and safety of ozone autohemotherapy combined with pharmacological therapy in postherpetic neuralgia

“…Oxygen-ozone therapy causes an increase in the rate of red blood cell glycolysis, causing the stimulation of 2,3-diphosphoglycerate, which leads to an increase in oxygen released to the tissues 13. It also causes a reduction in NADH and helps to oxidize cytochrome C 14. It increases the production of interferon, tumor necrosis factor, and interleukin-2, activating the immune system 15.…”