Effects of oxidative stress on sex-specific gene expression in the copepod Tigriopus californicus revealed by single individual RNA-seq

Li, Ning; Arief, Natasha; Edmands, Suzanne

doi:10.1016/j.cbd.2019.100608

Cited by 17 publications

(20 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the measured DNA damage is representative of endogenous ROS levels, male and females may experience different levels of oxidative damage throughout their lives. In T. californicus, gene expression was more greatly affected by sex differences than exogenous oxidant exposure, where male copepods differentially expressed more genes than females [51,52], including the upregulation of antioxidant associated genes. Elevated endogenous ROS levels could result in the male-specific hormetic response especially if the ROS is mitochondrially derived.…”

Section: Discussionmentioning

confidence: 99%

“…Foley et al [50] reported no difference in sex-specific longevity except in one replicate at high temperature where median longevity was slightly higher in females. Also, when challenged by both chronic [51] and acute [52] exposure to exogenous oxidative stress, males and females had divergent transcriptomic responses, suggesting that longevity and age-related phenotypes may differ between the sexes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mitonuclear interactions alter sex-specific longevity in a species without sex chromosomes

Flanagan

Edmands

2021

Proc. R. Soc. B.

Self Cite

View full text Add to dashboard Cite

Impaired mitochondrial function can lead to senescence and the ageing phenotype. Theory predicts degenerative ageing phenotypes and mitochondrial pathologies may occur more frequently in males due to the matrilineal inheritance pattern of mitochondrial DNA observed in most eukaryotes. Here, we estimated the sex-specific longevity for parental and reciprocal F1 hybrid crosses for inbred lines derived from two allopatric Tigriopus californicus populations with over 20% mitochondrial DNA divergence. T. californicus lacks sex chromosomes allowing for more direct testing of mitochondrial function in sex-specific ageing. To better understand the ageing mechanism, we estimated two age-related phenotypes (mtDNA content and 8-hydroxy-20-deoxyguanosine (8-OH-dG) DNA damage) at two time points in the lifespan. Sex differences in lifespan depended on the mitochondrial and nuclear backgrounds, including differences between reciprocal F1 crosses which have different mitochondrial haplotypes on a 50 : 50 nuclear background, with nuclear contributions coming from alternative parents. Young females showed the highest mtDNA content which decreased with age, while DNA damage in males increased with age and exceed that of females 56 days after hatching. The adult sex ratio was male-biased and was attributed to complex mitonuclear interactions. Results thus demonstrate that sex differences in ageing depend on mitonuclear interactions in the absence of sex chromosomes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitonuclear interactions alter sex-specific longevity in a species without sex chromosomes

Flanagan

Edmands

2021

Proc. R. Soc. B.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sex-biased gene expression under oxidative stress was also identified in copepod Tigriopus californicus. More specifically, females were found to display a more targeted response to oxidative stress by differentially expressing fewer genes but with a greater magnitude of fold change [147].…”

Section: Mitonuclear Communication In Homeostasis and Stressmentioning

confidence: 99%

Mitonuclear Interactions in the Maintenance of Mitochondrial Integrity

Karakaidos

Ράμπιας

2020

Life

View full text Add to dashboard Cite

In eukaryotic cells, mitochondria originated in an α-proteobacterial endosymbiont. Although these organelles harbor their own genome, the large majority of genes, originally encoded in the endosymbiont, were either lost or transferred to the nucleus. As a consequence, mitochondria have become semi-autonomous and most of their processes require the import of nuclear-encoded components to be functional. Therefore, the mitochondrial-specific translation has evolved to be coordinated by mitonuclear interactions to respond to the energetic demands of the cell, acquiring unique and mosaic features. However, mitochondrial-DNA-encoded genes are essential for the assembly of the respiratory chain complexes. Impaired mitochondrial function due to oxidative damage and mutations has been associated with numerous human pathologies, the aging process, and cancer. In this review, we highlight the unique features of mitochondrial protein synthesis and provide a comprehensive insight into the mitonuclear crosstalk and its co-evolution, as well as the vulnerabilities of the animal mitochondrial genome.

show abstract

“…This result indicates a sex-specific response to exposure to TEB at the concentration and exposure duration tested in the present work, which is consistent with findings from previous studies on copepods. A higher elevated transcriptomic response in males than in females to endocrine disruptor pesticides, oxidative stress, and cadmium (Cd) has been reported in the copepods E. affinis, Tigriopus californicus, and Pseudodiaptomus annandalei, respectively [33,[54][55][56]. Male copepods have been found to be more sensitive to stress for a range of stressors including temperature, salinity, and pollutants such as Cd at higher biological levels [57][58][59].…”

Section: Sex-specific Transcriptomic Responsementioning

confidence: 99%

Susceptibility of the Non-Targeted Crustacean Eurytemora affinis to the Endocrine Disruptor Tebufenozide: A Transcriptomic Approach

Arcanjo

Trémolet

Giusti-Petrucciani

et al. 2021

Genes

View full text Add to dashboard Cite

Copepods are zooplanktonic crustaceans ubiquitously widespread in aquatic systems. Although they are not the target, copepods are exposed to a wide variety of pollutants such as insect growth regulators (IGRs). The aim of this study was to investigate the molecular response of a non-targeted organism, the copepod Eurytemora affinis, to an IGR. Adult males and females were exposed to two sub-lethal concentrations of tebufenozide (TEB). Our results indicate a sex-specific response with a higher sensitivity in males, potentially due to a differential activation of stress response pathways. In both sexes, exposure to TEB triggered similar pathways to those found in targeted species by modulating the transcription of early and late ecdysone responsive genes. Among them were genes involved in cuticle metabolism, muscle contraction, neurotransmission, and gametogenesis, whose mis-regulation could lead to moult, locomotor, and reproductive impairments. Furthermore, genes involved in epigenetic processes were found in both sexes, which highlights the potential impact of exposure to TEB on future generations. This work allows identification of (i) potential biomarkers of ecdysone agonists and (ii) further assessment of putative physiological responses to characterize the effects of TEB at higher biological levels. The present study reinforces the suitability of using E. affinis as an ecotoxicological model.

show abstract

Effects of oxidative stress on sex-specific gene expression in the copepod Tigriopus californicus revealed by single individual RNA-seq

Cited by 17 publications

References 66 publications

Mitonuclear interactions alter sex-specific longevity in a species without sex chromosomes

Mitonuclear interactions alter sex-specific longevity in a species without sex chromosomes

Mitonuclear Interactions in the Maintenance of Mitochondrial Integrity

Susceptibility of the Non-Targeted Crustacean Eurytemora affinis to the Endocrine Disruptor Tebufenozide: A Transcriptomic Approach

Contact Info

Product

Resources

About