Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions

Zhao, Li; Zheng, Yu; Ma, Xiaochun; Wei, Dongzhi

doi:10.1007/s00449-008-0250-4

Cited by 42 publications

(29 citation statements)

References 32 publications

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“…Currently, glycerol is produced as a surplus by-product in the growing oleochemical industries for the production of soaps, fatty acids, waxes and surfactants [17]. Therefore, in an attempt to utilize glycerol as a starting material for microbial fermentations, several environmentally friendly bioprocesses have been proposed for the production of b-hydroxypropionaldehyde (reuterin) [18], 3-hydroxypropionic acid [19],2,3-butanediol [20] and 1,3-propandiol [21,22], and PHB [23].…”

Section: Introductionmentioning

confidence: 99%

Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol

Lee

Chang

2009

Bioprocess Biosyst Eng

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Succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown in a glycerol-fed continuous bioreactor in order to investigate the physiological responses of the cell to different pH values (5.9, 6.2, or 6.5) and various dilution rates, D. In these experiments, A. succiniciproducens showed a pH-dependent glycerol consumption behavior. When pH was maintained at 5.9 or 6.5, glycerol started to accumulate even at a very low D of 0.027 h(-1). Succinic acid yield was not significantly affected by the pH of the culture or the Ds. However, more acetic acid formation was observed when the growth rate of A. succiniciproducens was fast on glycerol at pH 6.2 (at D > or = 0.15 h(-1)). The highest obtainable succinic acid/acetic acid ratio was 40:1, which was 10 times higher than that obtained by batch cultures grown on glucose. The maximum obtainable productivity of succinic acid was 2.1 g L(-1) h(-1)), which was 14 times higher than that obtained by batch culture.

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Section: Introductionmentioning

confidence: 99%

Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol

Lee

Chang

2009

Bioprocess Biosyst Eng

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“…Some of the studies presented statistical optimization of fermentation parameters [10,15]. Others reports describe the use of metabolic engineering tools, purposed at metabolic flux manipulation by oxidative route interruptions [13] or by over expression of enzymes relevant to 1,3-PD synthesis [4]. A comparatively simple, but with great influence on the glycerol fermentation approach was used in our work.…”

Section: Discussionmentioning

confidence: 98%

“…The most significant factors, affecting the fermentation are the medium content, the aeration regime, and pH. The first two influencing factors were a subject of detailed research by a number of scientific experiments [4][5][6][7] and were optimized to a great extend. In contrast, pH requirements for predominant 1,3-PD production and low by-products receiving at the same time, still to be obscure until now [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations

Petrov

Stoyanov

2011

Bioprocess Biosyst Eng

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1,3-Propanediol (1,3-PD) is a bivalent alcohol, used in a number of chemical syntheses. It could be produced from glycerol in course of microbial fermentation by Klebsiella pneumoniae along with more than five minor liquid products. With the purpose to enhance 1,3-PD production and to eliminate by-products formation, principally new pH control on the process was applied. The method, named "forced pH fluctuations" was realized by consecutive raisings of pH with definite ΔpH amplitude (ranging from 1.0 to 2.0) at time intervals between 2 and 4 h, during a series of fed batch processes. The fermentation performed by forced pH fluctuations with ΔpH = 1.0, risen at every 3 h was evaluated as the most successful. Increase by 10% of the maximal amount of 1,3-PD (g/l), 22% higher productivity [g/(l h)], and 29% increase in 1,3-PD molar yield were achieved, compared to the referent fed batch (with constant pH = 7.0). In addition, significant decrease in by-products formation was obtained. The most important reduction was observed in the lactic and acetic acids yields, where 50 and 70% decrease were reached. The results suggested the potential of pH to manage the share and quantity of product spectrum in mixed diols-acids fermentations. The application of "forced pH fluctuations method" achieves the desirable increase in 1,3-PD formation and decrease in by-products accumulation at the same time by a comparatively simple approach by adjustment of one bioprocess parameter only.

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“…After culturing for several generations, the titre or yield and the activity of key enzymes of 10-100 passages were still identical to that of passage 0, which indicated that the recombinants had good genetic stability. However, in the study of some strains like Kp-M1 and Kp-M7 (69), their longevity profile was not very good (70,71). In order to increase their stability and make their repeated or continued use possible, many researchers adopted different methods to maintain the biological activity of engineered strains.…”

Section: Genetic Stability Of Engineered Strainsmentioning

confidence: 99%

“…The most common are batch and fed-batch fermentation. Production parameters of 1,3-PD are given in Table 3 (36,70,(72)(73)(74)(75).…”

Section: Different Fermentation Approachesmentioning

confidence: 99%

Genetically Engineered Strains: Application and Advances for 1,3-Propanediol Production from Glycerol

Yang

Yun

Zhang

et al. 2017

Food Technol. Biotechnol.

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SUMMARY1,3-Propanediol (1,3-PD) is one of the most important chemicals widely used as monomers for synthesis of some commercially valuable products, including cosmetics, foods, lubricants and medicines. Although 1,3-PD can be synthesized both chemically and biosynthetically, the latter offers more merits over chemical approach as it is economically viable, environmentally friendly and easy to carry out. The biosynthesis of 1,3-PD can be done by transforming glycerol or other similar substrates using some bacteria, such as Clostridium butyricum and Klebsiella pneumoniae. However, these natural microorganisms pose some bottlenecks like low productivity and metabolite inhibition. To overcome these problems, recent research efforts have been focused more on the development of new strains by modifying the genome through different techniques, such as mutagenesis and genetic engineering. Genetically engineered strains obtained by various strategies cannot only gain higher yield than wild types, but also overcome some of the barriers in production by the latter. This review paper presents an overview on the recent advances in the technological approaches to develop genetically engineered microorganisms for efficient biosynthesis of 1,3-PD.

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Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions

Cited by 42 publications

References 32 publications

Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol

Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol

Accelerated production of 1,3-propanediol from glycerol by Klebsiella pneumoniae using the method of forced pH fluctuations

Genetically Engineered Strains: Application and Advances for 1,3-Propanediol Production from Glycerol

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