Kinetic study on succinic acid and acetic acid formation during continuous cultures of Anaerobiospirillum succiniciproducens grown on glycerol

Abstract

Succinic acid-producing Anaerobiospirillum succiniciproducens was anaerobically grown in a glycerol-fed continuous bioreactor in order to investigate the physiological responses of the cell to different pH values (5.9, 6.2, or 6.5) and various dilution rates, D. In these experiments, A. succiniciproducens showed a pH-dependent glycerol consumption behavior. When pH was maintained at 5.9 or 6.5, glycerol started to accumulate even at a very low D of 0.027 h(-1). Succinic acid yield was not significantly affected by the pH of the culture or the Ds. However, more acetic acid formation was observed when the growth rate of A. succiniciproducens was fast on glycerol at pH 6.2 (at D > or = 0.15 h(-1)). The highest obtainable succinic acid/acetic acid ratio was 40:1, which was 10 times higher than that obtained by batch cultures grown on glucose. The maximum obtainable productivity of succinic acid was 2.1 g L(-1) h(-1)), which was 14 times higher than that obtained by batch culture.

Conversion of agroindustrial residues for high poly(γ-glutamic acid) production by Bacillus subtilis NX-2 via solid-state fermentation

“…Waste glycerol is co-produced at a weight ratio of approximately 10% of biodiesel as a primary byproduct, which is impure and shows low economic value (Da Silva et al, 2009). In fermentation, waste glycerol as a carbon source can be converted to various value-added products, such as 3-hydroxypropionic acid (Raj et al, 2008) and succinic acid (Lee et al, 2010). In this present study, waste glycerol was targeted for γ-PGA production via SSF by B.…”

A complete industrial system for economical succinic acid production by Actinobacillus succinogenes

“…Among succinic acid producers such as Actinobacillus succinogenes (Guettler et al, 1999), Anaerobiospirillum succiniciproducens (Lee et al, 2010), Mannheimia succiniciproducens (Lee et al, 2002), and recombinant Escherichia coli (Jantama et al, 2008), A. succinogenes not only could efficiently produce succinate (Mckinlay et al, 2007), but also could use a wide range of carbon sources, including lactose, xylose, arabinose, cellobiose, and other reduced sugars, to produce succinic acid as the major end product (Guettler et al, 1999;Van der Werf et al, 1997). The cost of carbon sources has been reduced by many renewable resources, such as straw (Zheng et al, 2009), corn fiber , crop stalk wastes (Li et al, 2010c), whey (Wan et al, 2008), wheat (Dorado et al, 2009), and cane molasses (Liu et al, 2008b), all of which could be hydrolyzed into mixed sugars.…”

Continuous succinic acid production from xylose by Actinobacillus succinogenes

“…Various microbial platforms have been explored for bio-production of SA with the best bench-scale performance achieved by Actinobacillus succinogenes [11][12][13][14], Anaerobiospirillum succiniciproducens [15,16], Mannheimia succiniciproducens [17][18][19], and engineered strains of Escherichia coli [20][21][22].…”