A complete industrial system for economical succinic acid production by Actinobacillus succinogenes

Engineered biosynthesis of biodegradable polymers

“…These mutants also produced less acetate and formic acid compared to the parent 130Z strain. There have been several efforts focused on replacing expensive nitrogen sources such as yeast extract with cheaper sources such as corn steep liquor [97]. McKinlay et al used chemically defined medium for succinic acid production and since this eliminates the use of complex medium, downstream separation and purification processes are much cheaper and simpler [118].…”

Bio-generation of succinic acid by fermentation of Physaria fendleri seed polysaccharides

“…6. It is understandable why most renewable approaches to succinic acid emphasize glucose as feedstock (Lee et al, 1999;Wu et al, 2012;Li et al, 2011;Leung et al, 2012), glucose is the most abundant renewable woody biomass. A recent approach has also added glycerol to the mix of metabolizable feedstock to succinic acid (Guettler et al, 1999).…”

“…Isolation of the generated succinate as succinic acid from the fermentation broth as described in the literature (de Baros et al, 2013;Song and Lee, 2006;Chen et al, 2011;Saha and Bothast, 1999;Hong et al, 2001;Tamada et al, 1990;Hong and Hong, 2000;Li, 2011) was markedly simplified and improved when the broth was directly stirred with dilute HCl to protonate the carboxylate salts in the mixture followed by direct continuous liquid-liquid extraction with diethyl ether at its boiling temperature (34 • C). Application of the partition law (Kim et al, 2004) in this procedure simplified removal of succinic acid into diethyl ether; the latter's immiscibility in water enabled quantitative extraction of the solutes, succinic acid, acetic and lactic acids from the aqueous milieu.…”

Metabolic capabilities of Actinobacillus succinogenes for succinic acid production

“…Moreover, many of these sources are waste made by other industries and reusing them will lead to reducing environmental pollution. Different sources has been used as renewable carbon substrate, including corn stalk and cotton stalk (Li et al, 2010), cane molasses (Liu et al, 2008), sugarcane baggase (Borges and Pereira, 2011), straw hydrolysate , corn stover (Zheng et al, 2010), sake lees (Chen et al, 2010), corn fiber (Li et al, 2011) and waste yeast hydrolysate (Chen et al, 2011). Many of these cheap renewable sources are mixtures of hexose and pentose after depolymerisation (Jeffries and Jin, 2004).…”