Effects of olive oil and its fractions on oxidative stress and the liver's fatty acid composition in 2,4-Dichlorophenoxyacetic acid-treated rats

Abstract

BackgroundOlive oil's beneficial effects are not only related to its high content of oleic acid, but also to the antioxidant potential of its polyphenols. In this study, we assess the effects of virgin olive oil and its fractions on 2,4-D- induced oxidative damage in the liver of rats.MethodsMale Wistar rats were randomly divided into eight groups of ten each: (C) a control group, (D) group that received 2,4-D (5 mg/kg b.w.), (D/EVOO) group treated with 2,4-D plus extra virgin olive oil, (D/OOHF) group that received 2,4-D plus hydrophilic fraction, (D/OOLF) group treated with 2,4-D plus lipophilic fraction, (EVOO) group that received only extra virgin olive oil, (OOHF) group given hydrophilic fraction and (OOLF) group treated with lipophilic fraction. These components were daily administered by gavage for 4 weeks.ResultsA significant liver damage was observed in rats treated with 2,4-D via increased serum levels of transaminases and alkaline phosphatase, hepatic lipid peroxidation and decreased hepatic antioxidant enzyme activities, namely, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase. The liver's fatty acid composition was also significantly modified with 2,4-D exposure. However, extra virgin olive oil and hydrophilic fraction intake during 2,4-D treatment induced a significant increase in the antioxidant enzyme activities and a decrease in the conjugated dienes (CD) and thiobarbituric acid-reactive substances (TBARs) levels in the liver. The lipophilic fraction supplemented to 2,4-D- treated rats did not show any improvement in the liver oxidative status while a marked improvement was detected in the hepatic fatty acid composition of rats supplemented with olive oil and the two fractions.ConclusionWe concluded that the protective effect of olive oil against oxidative damage induced by 2,4-D is mainly related to the antioxidant potential of its hydrophilic fraction.

High Fatty Diet Effects on Rat Liver

“…Nakbi et al (27) concluded that protective effect of oleic acid against oxidative damage (26). These findings clarifies that how a high-fat diet protects the liver in our study.…”

“…Gomez-Lechona et al (28), designed a study to define an experimental model of hepatocellular steatosis, and found that a high proportion of palmitic acid (oleate/ palmitate, 0:3 ratio) might represent a cellular model of steatosis (27).…”

Analysis of toxic, antidiabetic and antioxidant potential of Bambusa balcooa Roxb. leaf extracts in alloxan-induced diabetic rats

“…Results were in accordance to previous works on other bamboo species (Goyal et al 2011a;Nagavani and Rao 2010). Mostly, the antioxidant activity in plants is due to the presence of polyphenols (Nakbi et al 2010;Middha et al 2013;Usha et al 2014). Moreover, the higher the inhibitory action, the more powerful is the antioxidant activity (Ajith 2010).…”

Olive oil protects against 2,4‐dichlorophenoxyacetic acid‐induced oxidative renal dysfunction in adult rats

“…Extra virgin olive oil and its fractions analysis were performed as reported in our previous paper [27]. In fact, the determination of fatty acids was accomplished through the quantification of their methyl esters (FAMEs) by gas chromatography using a model 5890 series II instruments (HewelettPackard Ca Palo Alto, California, USA) as described in ref.…”

“…The olive oil lipophilic fraction (OOLF) was obtained from EVOO as follows: the EVOO was homogenized for 1 min with water (1:1, v/v), and the oil was separated by centrifugation; this procedure was repeated six times. Then, the oil fraction (OOLF) was filtered through a cellulose acetate membrane [26,27].…”