Effects of Oligosaccharides on Galactosamine Hepatitis in Rats.

Wang, Binbin; Chen, Cheng; Egasira, Yukari; Ohta, Takeo; Sanada, Hiroo

doi:10.4327/jsnfs.48.181

Cited by 10 publications

(3 citation statements)

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“…8,9,[23][24][25] Some investigators have also observed that liver weight decreases 24 h after GalN injection as a result of atrophy followed by necrosis. 26,27) In this study, serum ALT, AST, and LDH activities that were increased by GalN treatment were significantly decreased by pretreated PPE. Furthermore, although there was no significant difference in liver weight between the two GalN-treated groups, the PPE + GalN group tended to increase in liver weight more than the control + GalN group.…”

Section: Discussionmentioning

confidence: 81%

Hepatoprotective Effects of Purple Potato Extract againstD-Galactosamine-Induced Liver Injury in Rats

Han

Hashimoto

Shimada

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 81%

Hepatoprotective Effects of Purple Potato Extract againstD-Galactosamine-Induced Liver Injury in Rats

Han

Hashimoto

Shimada

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…On the other hand, it has been reported that GalN causes a decrease in liver weight as a result of atrophy followed by necrosis in rats. 20,21) This phenomenon might originate in hepatocellular injury secondary to loss of proteins, because GalN reduces the intracellular pool of uracil nucleotide in hepatocytes, thus inhibiting RNA and protein synthesis. 22) As Table 1 shows, liver weight in the GalN-treated groups was significantly (p < 0:05) lower than in the control group.…”

Section: )mentioning

confidence: 99%

Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Han

Hashimoto

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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The protective effects of red potato extract (RPE) as to liver damage were determined in D-galactosamine (GalN)-intoxicated rats. Increases in serum aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase activities, all of which were induced by GalN injection, decreased in RPE administered rats, suggesting that RPE acts as a functional food showing anti-hepatotoxicity.Key words: colored potato; anthocyanin; D-galactosamine; hepatotoxicity; rat It is well-known that polyphenols originating from various fruits and vegetables have a wide range of biological effects such as antioxidant, anti-inflammatory, and anti-carcinogenic activities.1,2) Generally, potato has not been regarded as a food rich in antioxidants, but several researchers have found that depending on the genetic variety, some potato contain considerable amounts of polyphenols, flavonoids, and anthocyanins.3,4) Furthermore, recently new varieties of potato have been developed around the world to find new uses, as these are rich in antioxidants. Among new potatoes, the colored potato especially has attracted special interest in many countries due to its color appeal, such as yellow, purple, or red. The pigments of colored potatoes have been identified as anthocyanin derivatives.3-6) Like natural antioxidants from other plants, antioxidants from potato are believed to behave as an important functional food, 4) but little information is available. Recently, we found that purple potato extract (PPE, Hokkai no.92) attenuates the liver damage induced by D-galactosamine (GalN) in rats, 7) but it has not yet been reported that different colored potato extracts have analogous health benefits. Hence in this study we investigated the hepatoprotective effects of red potato extract (RPE, Hokkai no.91) against GalNinduced liver injury in rats.RPE was prepared according to our previous report. 7)Briefly, pared potato was homogenized with 5% formic acid and centrifuged at 2;000 Â g for 20 min at 4 C. Finally, the supernatant fraction was lyophilized and dissolved in 70% ethanol or distilled water (RPE). The total polyphenol content in the ethanol solution was determined by the Folin-Ciocalteu method.8) Absorbance was measured at 750 nm using a spectrophotometer (Shimadzu 1600-UV, Kyoto, Japan). The flavonoid content in the ethanol solution was determined according to the report of Jia et al., 9) using standards based on the known (+)-catechin concentration. Briefly, 200 ml of a known concentration of extract was mixed with 1.25 ml of distilled water and 75 ml of 5% NaNO 2 , and then 150 ml of 10% AlCl 3 was added 6 min later. Next, 500 ml of 1 N NaOH was added, and the total was made up to 2.5 ml with distilled water. Absorbance was measured at 510 nm using a spectrophotometer. The monomeric anthocyanin content in aqueous solution was measured by a pH differential method using a spectrophotometer.10) It was calculated using the molar extinction coefficient of pelargonidin-3-glucoside (31,600 l cm À1 mg À1 ), a molecular weight of 433.2 g mol À...

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“…25 Protective effects on galactosamine-induced liver damage have been reported for green tea and dietetic fibers, although the mechanism of such protection has not been explained. 41,42 D-003 has been found to be effective for preventing liver damage induced with agents like CCl 4 and paracetamol, 39,40 whose hepatotoxicity has been linked to contributions by augmented LP and free radicals. [16][17][18][19][20][21][22][23][24] In fact, the hypothesis underlying the rationale for investigating the putative protection of D-003 on the liver damage induced with both paracetamol and CCl 4 was based in the effective antioxidant profile of D-003 to prevent LP induced with several agents in rats 34 and humans.…”

Section: Discussionmentioning

confidence: 98%

Lack of Protective Effect of D-003, a Mixture of High-Molecular-Weight Primary Acids from Sugar Cane Wax, on Liver Damage Induced by Galactosamine in Rats

Noa

Mendoza

Más

et al. 2005

Journal of Medicinal Food

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D-003 is a mixture of very-high-molecular-weight aliphatic primary acids purified from sugar cane wax, wherein octacosanoic acid is the most abundant. Experimental and clinical studies have shown that D-003 lowers cholesterol and prevents plasma lipoprotein peroxidation (LP). D-003 has protected against the histological changes characteristic of CCl4- and paracetamol-induced hepatic injury in rats, in which LP plays a pivotal role for explaining the resulting hepatotoxicity. Galactosamine induces hepatotoxicity associated with depressed RNA and protein synthesis, not with LP. The aim of this study was to evaluate whether D-003 could prevent hepatoxicity induced by mechanisms others than increased LP. We investigated the effects on galactosamine hepatotoxicity in rats distributed into five groups: a negative control group, a positive control group, and three groups treated with galactosamine and D-003 (5, 25, and 100 mg/kg). To induce liver damage, galactosamine (800 mg/kg) was injected intraperitoneally 30 minutes after dosing with vehicle or D-003. Twenty-four hours later, rats were sacrificed, and livers were immediately removed for histopathological studies. Livers from positive controls showed the characteristic pattern of galactosamine-induced damage. Galactosamine significantly reduced the percentage of normal hepatocytes, increasing both necrotic or lipid-rich hepatocytes compared with negative controls. D-003, however, did not increase the percentage of normal hepatocytes compared with positive controls, indicating that treatment was not effective for preventing the hepatic injury induced with galactosamine. Likewise, D-003 failed to change the content of necrotic and lipid-rich hepatocytes relative to positive controls. It is concluded that D-003 did not protect against the histological changes of galactosamine-induced hepatotoxicity.

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Effects of Oligosaccharides on Galactosamine Hepatitis in Rats.

Cited by 10 publications

References 0 publications

Hepatoprotective Effects of Purple Potato Extract againstD-Galactosamine-Induced Liver Injury in Rats

Hepatoprotective Effects of Purple Potato Extract againstD-Galactosamine-Induced Liver Injury in Rats

Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Lack of Protective Effect of D-003, a Mixture of High-Molecular-Weight Primary Acids from Sugar Cane Wax, on Liver Damage Induced by Galactosamine in Rats

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