Hepatoprotective Effects of Purple Potato Extract against<scp>D</scp>-Galactosamine-Induced Liver Injury in Rats

Han, Kyu‐Ho; Hashimoto, Naoto; Shimada, Kaoru; Sekikawa, Mitsuo; Noda, Takahiro; Yamauchi, Hiroaki; Hashimoto, Makoto; Chiji, Hideyuki; Topping, David L.; Fukushima, Michihiro

doi:10.1271/bbb.50670

Cited by 49 publications

(37 citation statements)

References 28 publications

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“…19) Previously, we reported that polyphenol-rich PPE attenuates the serum aminotransferases and LDH activities of GalN-induced hepatotoxicity in rats. 7) In the present study, we found that serum ALT, AST, and LDH activities in the RPE + GalN group decreased dramatically more than those in the control + GalN group ( Table 1). In view of these data, RPE might have preventive effects as to GalN liver injury in rats.…”

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confidence: 57%

“…[3][4][5][6] Like natural antioxidants from other plants, antioxidants from potato are believed to behave as an important functional food, 4) but little information is available. Recently, we found that purple potato extract (PPE, Hokkai no.92) attenuates the liver damage induced by D-galactosamine (GalN) in rats, 7) but it has not yet been reported that different colored potato extracts have analogous health benefits. Hence in this study we investigated the hepatoprotective effects of red potato extract (RPE, Hokkai no.91) against GalNinduced liver injury in rats.…”

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confidence: 99%

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Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Han

Hashimoto

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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The protective effects of red potato extract (RPE) as to liver damage were determined in D-galactosamine (GalN)-intoxicated rats. Increases in serum aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase activities, all of which were induced by GalN injection, decreased in RPE administered rats, suggesting that RPE acts as a functional food showing anti-hepatotoxicity.Key words: colored potato; anthocyanin; D-galactosamine; hepatotoxicity; rat It is well-known that polyphenols originating from various fruits and vegetables have a wide range of biological effects such as antioxidant, anti-inflammatory, and anti-carcinogenic activities.1,2) Generally, potato has not been regarded as a food rich in antioxidants, but several researchers have found that depending on the genetic variety, some potato contain considerable amounts of polyphenols, flavonoids, and anthocyanins.3,4) Furthermore, recently new varieties of potato have been developed around the world to find new uses, as these are rich in antioxidants. Among new potatoes, the colored potato especially has attracted special interest in many countries due to its color appeal, such as yellow, purple, or red. The pigments of colored potatoes have been identified as anthocyanin derivatives.3-6) Like natural antioxidants from other plants, antioxidants from potato are believed to behave as an important functional food, 4) but little information is available. Recently, we found that purple potato extract (PPE, Hokkai no.92) attenuates the liver damage induced by D-galactosamine (GalN) in rats, 7) but it has not yet been reported that different colored potato extracts have analogous health benefits. Hence in this study we investigated the hepatoprotective effects of red potato extract (RPE, Hokkai no.91) against GalNinduced liver injury in rats.RPE was prepared according to our previous report. 7)Briefly, pared potato was homogenized with 5% formic acid and centrifuged at 2;000 Â g for 20 min at 4 C. Finally, the supernatant fraction was lyophilized and dissolved in 70% ethanol or distilled water (RPE). The total polyphenol content in the ethanol solution was determined by the Folin-Ciocalteu method.8) Absorbance was measured at 750 nm using a spectrophotometer (Shimadzu 1600-UV, Kyoto, Japan). The flavonoid content in the ethanol solution was determined according to the report of Jia et al., 9) using standards based on the known (+)-catechin concentration. Briefly, 200 ml of a known concentration of extract was mixed with 1.25 ml of distilled water and 75 ml of 5% NaNO 2 , and then 150 ml of 10% AlCl 3 was added 6 min later. Next, 500 ml of 1 N NaOH was added, and the total was made up to 2.5 ml with distilled water. Absorbance was measured at 510 nm using a spectrophotometer. The monomeric anthocyanin content in aqueous solution was measured by a pH differential method using a spectrophotometer.10) It was calculated using the molar extinction coefficient of pelargonidin-3-glucoside (31,600 l cm À1 mg À1 ), a molecular weight of 433.2 g mol À...

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confidence: 57%

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Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Han

Hashimoto

et al. 2006

Bioscience, Biotechnology, and Biochemistry

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“…In a preliminary study, however, an anthocyanin-rich fraction separated from BE by a column chromatography was assayed with a cell viability test to determine the influence of the diluent on the antioxidant system, and we found the anthocyanin-rich fraction effective (data not shown). These data did not contradict those of our previous studies (3,16). Meanwhile, contents and compositions of anthocyanins and PACs in BE will differ depending on year, area, race, and other factors, suggesting that repeatability and reproducibility should be assessed in further studies.…”

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confidence: 72%

Black Soybean Seed Coat Extract Prevents Hydrogen Peroxide-Mediated Cell Death via Extracellular Signal-Related Kinase Signalling in HepG2 Cells

Hashimoto

Oki

Sasaki

et al. 2015

J Nutr Sci Vitaminol

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Summary Oxidative stress reduces cell viability and contributes to disease processes. Flavonoids including anthocyanins and proanthocyanidins reportedly induce intracellular antioxidant defence systems. Thus, in this study, we examined the antioxidant effects of a commercial extract from black soybean seed coats (BE), which are rich in anthocyanin and proanthocyanidin, and investigated the associated intracellular mechanisms in HepG2 cells. HepG2 cells treated with hydrogen peroxide (HPO) showed 60% viability, whereas pretreatment with BE-containing media for 2 h ameliorated HPO-mediated cell death by up to 90%. Pretreatment with BE for 2 h partially blocked HPO-mediated activation of ERK in HepG2 cells, and that for 1 h led to a 20% increase in intracellular total protein phosphatase (PP) activity, which is known to deactivate protein kinases. These results indicate that BE prevents HPO-mediated cell damage by inhibiting ERK signalling, potentially via PPs.

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“…EA is known to possess a variety of biological activities including potent antioxidant anticancer and antimutagen properties (Hassoun et al 1997). Even though the exact molecular mechanism of EA remains unknown, its potent scavenging action on OH and (O 2 -) might be responsible for these effects (Han et al 2006). All these taken into account, the present study aims to investigate the therapeutic and protective effects of EA on D-GalN-induced kidney damage in rats.…”

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confidence: 99%

Protective effects of ellagic acid in d-galactosamine-induced kidney damage in rats

et al. 2015

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D-Galactosamine (D-GalN), which is an established experimental toxin, primarily causes liver injury by the generation of free radicals and depletion of UTP nucleotides. D-GalN intoxication also induces renal dysfunction thus, renal failure is often associated with the end-stage of the liver damage. We have investigated both preventive and curative effects of ellagic acid (EA) in this study. EA treatment at a gavage dose of 20 mg/kg body weight was administered before and after intraperitoneal (i.p.) injection of D-GalN at a dose of 750 mg/kg. Tissue and blood samples of animals were collected for morphological and biochemical evaluations. Our study results suggest that EA treatment both prior to and after the toxin administration successfully altered the toxic effects on the rats. Moreover, pre-treatment of EA was more protective than post-treatment indicated by histopathological and biochemical values. In conclusion, EA treatment both before and after D-GalN intoxication could protect kidney tissues against D-GalN induced oxidative stress.

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Hepatoprotective Effects of Purple Potato Extract againstD-Galactosamine-Induced Liver Injury in Rats

Cited by 49 publications

References 28 publications

Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Red Potato Extract Protects fromD-Galactosamine-induced Liver Injury in Rats

Black Soybean Seed Coat Extract Prevents Hydrogen Peroxide-Mediated Cell Death via Extracellular Signal-Related Kinase Signalling in HepG2 Cells

Protective effects of ellagic acid in d-galactosamine-induced kidney damage in rats

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