Effects of NAA and BAP, Double-Layered Media, and Light Distance on<i>In Vitro</i>Regeneration of<i>Nelumbo nucifera</i>Gaertn. (Lotus), an Aquatic Edible Plant

Mahmad, Noraini; Taha, Rosna Mat; Othman, Rashidi; Saleh, Abd. Latif; Hasbullah, Nor Azlina; Elias, Hashimah

doi:10.1155/2014/745148

Cited by 12 publications

(11 citation statements)

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“…Lotus seeds are regarded as a wellknown health food for protein, amino acids, vitamins, unsaturated fatty acids. Its seeds are also used as a folk medicine to treat tissue inflammation, cancer, diuretics and skin disease (Bhat and Sridhar 2008;Liu et al 2014;Mahmad et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In rice, its peak viscosity, as well as breakdown of paste increased within the first 2 months, but decreased after the 6 th month (Tananuwong and Malila 2011;Shukla et al 2014). As for sorghum and maize, areas of the endosperm became hard, because the solubility of proteins decreased, as well as the interactions between starch and protein (McDonough et al 2004). Similarly, amylose activity and protein digestibility of wheat grain decreased as the storage time increased (Oladunmoye et al 2014;Wang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Chemical Deterioration of Lotus Seeds During Storage

Luo

Liu

Huang

et al. 2016

Journal of Food Quality

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Starch, protein and lipids, the major components in lotus seeds, affected taste and cooking properties of the food. The chemical compositions, gelatinization properties, crystal structure of the starch, compositions of fatty acids and soluble protein of lotus seeds, stored for 24 months, 12 months and as fresh were studied. The overall starch, protein and lipid contents in lotus seeds remained unchanged during storage, but structural changes occurred. The contents of amylose and free fatty acid increased significantly during prolonged storage, but the solubility of protein decreased dramatically. The increases of onset temperature, peak temperature and gelatinization enthalpy of lotus seed starch were also studied by a differential scanning calorimetry. Its content especially for disulfide bonds, increased from 63.24 to 95.59 μ mol/g. All changes had significant correlation with storage time and ultimately denatured the sensory quality of selected aged lotus seeds. The study could be conductive to fresh lotus seed discrimination from stale seed, and dynamic monitoring of lotus seed, by determination chemical deterioration of lotus seeds during storage. Practical Applications This study is helpful to discriminate fresh lotus seed from stale seed, and explain aging mechanism, and put forward the improvement measures of lotus seed storage conditions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chemical Deterioration of Lotus Seeds During Storage

Luo

Liu

Huang

et al. 2016

Journal of Food Quality

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“…Furthermore, rhizomes of specimens grown in in vitro conditions required a longer time to reach their full developmental stage or maturity, even though both specimens were collected at the same plant age. According to Mahmad et al [ 35 ], in vitro lotus plants ( Nelumbo nucifera Gaertn.) grew vigorously and possessed similar physiological characteristics as the mother plant only after 8 months of acclimatization.…”

Section: Resultsmentioning

confidence: 99%

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

Wafa

Taha

Mohajer

et al. 2016

BioMed Research International

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An efficient protocol for micropropagation of Canna indica L., an economically and pharmaceutically important plant, was standardized using rhizome explants, excised from two-month-old aseptic seedlings. Complete plant regeneration was induced on MS medium supplemented with 3.0 mg/L BAP plus 1.5 mg/L NAA, which produced the highest number of shoots (73.3 ± 0.5%) and roots (86.7 ± 0.4%) after 2 weeks. Furthermore, the optimum media for multiple shoots regeneration were recorded on MS enriched with 7.0 mg/L BAP (33.0 ± 0.5%). Plantlets obtained were transplanted to pots after two months and acclimatized in the greenhouse, with 75% survival. In addition, ultrastructural studies showed that rhizomes of in vitro grown specimens were underdeveloped compared to the in vivo specimens, possibly due to the presence of wide spaces. Meanwhile, the leaves of in vivo specimens had more open stomata compared to in vitro specimens, yet their paracytic stomata structures were similar. Hence, there were no abnormalities or major differences between in vitro regenerants and mother plants.

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“…Hence, there are predominant advantages of in vitro culture compared with the traditional propagation methods (Borah et al, 2019). Although there are studies on in vitro propagation of lotus (Shou et al, 2008); (Mahmad et al, 2014). There is no study describing such studies on lotus in Vietnam especially Red Lotus variety or Vinh Thanh Red Lotus.…”

Section: In Vitro Propagation Of Red Lotus (Nelumbo Nucifera Gaertn) mentioning

confidence: 99%

“…The seed needs to be carried out by scarifying the hard seed coat and then incubating at 25  -30  C. Seeds should be submerged in a water saturated media. If the hard coat remains intact, the seed will remain viable for centuries and it may take a few years for the seed to sprout if placed in water (Mahmad et al, 2014). However, propagation by seed will not be true-to-type as lotus has a high degree of genetic variability (Nguyen, 2001).…”

Section: Introductionmentioning

confidence: 99%

In vitro Propagation of Red Lotus (Nelumbo nucifera Gaertn) - An Aquatic Edible Plant in Vietnam

Trang

Avtar

Hương

et al. 2020

ASD

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Background: Lotus is an important aquatic plant with great economic value, not only as an ornamental flower but also as a source of herbal medicine. In general, lotus is usually propagated through the rhizome or tuber but the normal propagation rate is very low and it often depends on the quality of water environment and the weather conditions where it is grown. Lotus propagation by tissue culture has many predominant advantages compared with the traditional propagation methods. The current study aims to study the in vitro propagation of Hue’s Red Lotus, a famous local lotus in Hue city, Vietnam. Methods: Fresh mature seeds of Red lotus (23 to 25 days old) were used as initial materials for in vitro propagation. Experiments are carried out to assess sterilization ability by HgCl2, 0.1% and determine the appropriate formula for shoot regeneration, multiple shoot clumps induction, root induction of multiple shoot clumps and acclimatization. Result: The optimum formula for seeds of Vinh Thanh Red Lotus is to sterilize surface of seeds by 70% ethanol for 30 seconds and then HgCl2 0.1% for 16 minutes. The samples were cultured on MS (Murashige Skoog) medium supplemented without plant growth regulators for in vitro shoot regeneration. MS medium added with 0.5 mg/L BAP gave the highest multiplication rate (5.60 shoots per explant) and good quality shoots. Adding 0.5 mg/L NAA in rooting medium provided the highest rooting effect and good root quality (18.17 roots/shoot clumps). The survival rate of transplanting plants was 40% on mud medium which were soaked for one week in 2g/L KMnO4 after two weeks in the greenhouse.

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Effects of NAA and BAP, Double-Layered Media, and Light Distance onIn VitroRegeneration ofNelumbo nuciferaGaertn. (Lotus), an Aquatic Edible Plant

Cited by 12 publications

References 11 publications

Chemical Deterioration of Lotus Seeds During Storage

Chemical Deterioration of Lotus Seeds During Storage

Organogenesis and Ultrastructural Features ofIn VitroGrownCanna indicaL.

In vitro Propagation of Red Lotus (Nelumbo nucifera Gaertn) - An Aquatic Edible Plant in Vietnam

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