Effects of microcurrent application and 670 nm InGaP low-level laser irradiation on experimental wound healing in healthy and diabetic Wistar rats

Neves, Lia Mara Grosso; Matheus, R L; Santos, Gláucia Maria Tech dos; Esquisatto, Marcelo Augusto Marretto; Amaral, Maria Esméria Corezola do; Mendonça, Fernanda Aparecida Sampaio

doi:10.1088/1054-660x/23/3/035604

Cited by 14 publications

(19 citation statements)

References 62 publications

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“…TGF-β1 is expressed at the beginning of the repair of injured tissues, when it exerts a chemoattractive effect on neutrophils, macrophages and fibroblasts to the inflammatory site [70]. Neves et al [51] observed an increase in TGF-β1 expression at the beginning of skin wound healing irradiated with laser and a decrease in late stages of repair excisional wounds in healthy and diabetic rats. The same was observed in our study.…”

Section: Discussionmentioning

confidence: 99%

“…For this purpose, the animals were immobilised without sedation and treated daily at the same time for 21 days. Daily treatment was chosen according to the literature and experimental designs previously conducted by our and other research groups [15,17,24,51,52].…”

Section: Methodsmentioning

confidence: 99%

“…The density energy used in this experimental model has been used by our research group with favourable results for tissue repair [15,51,52], and the literature reports that different authors used similar energy density [13,24,25,53]. Non-contact laser irradiation was performed punctually, in the continuous mode, at a distance of ±2 mm and an angle of 90°in relation to the wound surface.…”

Section: Methodsmentioning

confidence: 99%

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InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Catarino¹,

Godoy²,

Scharlack³

et al. 2015

Lasers Med Sci

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“…Two different types of laser (670-InGaP and 830-nm GaAlAs) are used for Chiarotto et al [8] in the treatment of second-degree burns in rats and it was observed that the laser 670-nm InGaP was more effective in increasing the numbers fibroblast at the injury site. Other authors have also demonstrated the beneficial effects of low intensity laser in tissue repair using 670nm [6,23,24].…”

Section: Introductionmentioning

confidence: 95%

Differing energy densities with laser 670 nm InGaP controls inflammation and collagen reorganization in burns

Maligieri

Neves

Morais

et al. 2017

Burns

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“…These results are in accordance with the literature that considers the laser as an angiogenesis stimulator [ 30 ]. Neves et al [ 31 ] studied the effect of laser irradiation 670-nm InGaP on excisional wounds in Wistar rats and observed that this approach also promoted an increase in angiogenesis at the injury site. Similarly, Sousa et al [ 32 ] observed significant angiogenic effects promoted by low-level laser therapy in skin burns and excisional wounds on the back of Wistar rats, respectively.…”

Section: Discussionmentioning

confidence: 99%

Activity of Porophyllum ruderale leaf extract and 670-nm InGaP laser during burns repair in rats

Jácomo¹,

Velozo²,

Lotti³

et al. 2015

BMC Complement Altern Med

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BackgroundIn this study, we investigated the effects of an extract of the leaves of Porophyllum ruderale and laser irradiation on the healing of burns.MethodsSeventy-two rats were divided in four groups: untreated controls, treated with laser irradiation, treated with P. ruderale and treated with both P. ruderale and laser irradiation. Burns were produced with a metal plate on the backs of the animals. Wound samples were collected for structural and morphometric analyses and to quantify the expression of TGF-β1 and VEGF.ResultsLaser irradiation increased the number of fibroblasts, collagen fibers and newly formed vessels and decreased the number of granulocytes at the site of the wounds. Densitometric analysis revealed a significant increase in the expression of TGFβ-1 in the wounds treated with laser irradiation and with the P. ruderale extract at the beginning of the healing process and a decreased during the experimental period. The expression of VEGF was highlighted in the lesions irradiated with laser alone.ConclusionInspite of not showing a beneficial effect on the laser combination with the P. ruderale extract, when the laser was used separately, a positive effects to enhance the healing of second-degree burns was promoted. P. ruderale was effective in decreasing the granulocytes during the repair process indicating a possible anti-inflammatory action of this extract of native flora, widely used in folk medicine, but little studied experimentally.

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Effects of microcurrent application and 670 nm InGaP low-level laser irradiation on experimental wound healing in healthy and diabetic Wistar rats

Cited by 14 publications

References 62 publications

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries

Differing energy densities with laser 670 nm InGaP controls inflammation and collagen reorganization in burns

Activity of Porophyllum ruderale leaf extract and 670-nm InGaP laser during burns repair in rats

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