Effects of methyl jasmonate and salicylic acid on the production of camphor and phenolic compounds in cell suspension culture of endemic Turkish yarrow(Achilleagypsicola) species

Abstract

Elicitors have been widely used as biotic and abiotic stimulants in triggering the production of secondary metabolites in plant cell culture. The present study aimed to enhance the production of camphor and phenolic compounds and cell growth using methyl jasmonate (MeJA) and salicylic acid (SA) in the cell suspension culture of Turkish endemic species Achillea gypsicola. Various concentrations (0, 10, 50, and 100 µM) of MeJA and SA were applied to 8-day-old cell cultures. The camphor and phenolic compound contents were determined using a headspace gas chromatographic-mass spectrometer device and spectrophotometer. Increasing doses of MeJA and SA significantly enhanced the accumulation of the camphor and phenolic compounds in general. The highest amount of camphor accumulation occurred in cells treated with 100 µM MeJA (0.3449 µg/g) and 50 µM SA (0.3816 µg/g). Increasing concentrations of MeJA resulted in a significant decrease in the total anthocyanin when compared to the initial culture. The present study showed that MeJA and SA could effectively be used as potent elicitors to enhance the production of camphor and phenolic compounds, along with cell growth, in cell suspension cultures of the endemic Turkish yarrow species Achillea gypsicola.

Improved Production of Industrially Important Essential Oils Through Elicitation in the Adventitious Roots of Artemisia amygdalina

“…Results revealed biosynthesis of the major bioactive compounds such as α-thujene (6.8%), α-pinene (8.3%), 1,8-cineole (16.2%), camphor (8.4%) and verbenole (10.2%) in the Me-J treated AR. Accordingly, methyl jasmonate (Me-J) has been reported for its influential role in the maximum accumulation of terpene volatiles in cell suspension cultures of Ajuga bracreosa and Achillea gypsicola , respectively [54,55]. Contrarily, lower values of essential oils were observed in shoot cultures of Sautreja khuzistanica in response to Me-J [56].…”

Designer nanoparticles for plant cell culture systems: Mechanisms of elicitation and harnessing of specialized metabolites

“…Metal and metal oxide NPs of silver, cobalt, and zinc oxide, copper oxide, titanium oxide, respectively, are the most widely studied group of nanoscale materials and are extensively reviewed elsewhere. [5][6][7][8][9][10] Synthesis and manipulation of the physicochemical characteristics of these are relatively simple, and therefore many studies have focused on this class as compared to the other types of NPs (Box 1). Table S1 provides further insights into the genetic, enzymatic, and reactive oxygen species (ROS) responses of plant cell culture system to these NPs.…”

“…Although elicitation with NPs in plant cell culture systems is currently an emerging field, recent years have shown a rapid increase in research activity in this area. The scope of this review differs from the available literature on nanoelicitation, [ 5–10 ] because it consolidates the information on nanoelicitors used specifically in plant cell culture systems, provides a critical analysis of their advantages, perceived mechanisms of elicitation, potential issues around cytotoxicity, and prospects of qualitative nanostructure‐activity relationship (QNAR) to optimize the synthesis of designer NPs for plant cell culture systems. It also sheds light on the possible routes to customize the NPs for hormone delivery and ways to reuse NPs to generate higher yields of specialized metabolites and reduce cost of nanoelicitation, respectively.…”

Comparison of Different Culture Media and Hormonal Concentrations for In-Vitro Propagation of Pomegranate

“…Micropropagation is a branch of applied science in the plant biotechnology, which recently has undergone a remarkable transformation and plays a decisive role in the propagation and breeding of species of trees, shrubs, and herbal plants. The advantages such as rapid propagation, genetic stability, and no limitation of time and space havemade this trend particularly important (Acikgoz et al, 2019;Guney, 2019). Today, studies have been done on using in-vitro culture techniques to propagate pomegranate.…”