Comparison of Different Culture Media and Hormonal Concentrations for In-Vitro Propagation of Pomegranate

Abstract

Pomegranate (Punica granatum) is one of the most popular fruits which is rich in nutrients and varied in cultivars including Hybrid, Wonderful (a sweet and delicious fruit with big size), and Seedless (sweet taste and very juicy). In this research, micropropagation of hybrid pomegranate cultivar (Wonderful and Seedless) was investigated. For the primary culture, the lateral and apical buds of pomegranate plant were used, and the plant materials were disinfected by Mercuric Chloride in the presence of Ascorbic Acid. Then disinfected explants cultivated to MS culture medium supplemented by 0.2 mg l −1 BAP and IBA 0.01 mgl −1 as an initial culture medium. For elongation and proliferation, BAP at five levels of 0, 0.2, 0.8, 1.4, and 2 mgl −1 , and IBA at two levels of 0 and 0.01 mgl −1 were used in woody plant medium (WPM). To determine the most suitable rooting medium, IBA was used at concentrations of 0, 0.5, and 1 mgl −1 in different culture media containg MS, WPM, and DKW. Different soil compositions were also used for the adaptation of plantlets. The results showed that the best concentration for Mercuric Chloride disinfectant was 0.1% and all the disinfection treatment times were appropriate. Moreover, woody plant medium with 1.4 mg l −1 of BAP without IBA was the best culture medium for proliferation. In rooting stage, using woody plant medium was detected as the most suitable too. In the adaptation stage, using Vermicompost, Perlite, and Cocopeat (1: 1: 1) was observed to be the best treatment.

Worthy Micropropagation Protocols of Seven Cultivars of Pomegranate (Punica Granatum L.) Cultivars in the Province of Duhok, Kurd Istan Rigion of Iraq.

“…In general, plantlets generate more roots when placed in a rooting media with low salt concentration (Drazeta, 1997). The optimum method for root inducing in pomegranates, according to various researchers, is to employ 0.5 mg/l NAA or IBA (Patil et al, 2011;Baghdad-Abadi et al, 2020;Maheswari et al, 2023 a,b). Contrarily, half-strength MS medium with 0.5 NAA or IBA demonstrated the highest adventitious rooting percentage in Punica granatum, which is consistent with the findings of this study (Desai et al, 2018;Bachake et al, 2019;Mulaei et al, 2020;Kabir et al, 2021).…”

Development and Optimization of a Rapid In Vitro Micropropagation System for the Perennial Vegetable Night Lily, Hemerocallis citrina Baroni

“…Cytokinins are mainly used to promote cell division and shoot regeneration from calli or organs [45]. Selecting PGR concentrations and the proper ratios of different PGRs is critical for tissue culture research [46]. TDZ is a highly effective cytokinin used in the tissue culture of various plants, while 6-BA, NAA and 2,4-D are often used in combination to induce adventitious buds or roots [47,48].…”

Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models

“…Plant tissue culture methods offer a promising approach for the rapid production of true-to-type pomegranate plants and the biotechnological exploitation of pomegranate and other plant species with valuable properties [5]. Previous studies have attempted to apply in vitro culture techniques to propagate different cultivars of pomegranate [6,7]. However, the findings have clearly emphasized that pomegranate micropropagation is moderately difficult and can vary depending on the cultivar, probably due to genetic variations among them [6,8].…”

“…The successful in vitro propagation of fruit trees is an intricate process that is influenced by numerous factors, including culture conditions, plant materials, and the composition of culture media, particularly PGRs [11]. Extensive research has emphasized the crucial role of PGRs, such as cytokinins and auxins, and their different combinations with gibberellic acid (GA 3 ) in promoting shoot regeneration in different pomegranate cultivars [7]. However, certain PGRs have shown varying levels of effectiveness in promoting proliferation.…”