Improved Production of Industrially Important Essential Oils Through Elicitation in the Adventitious Roots of Artemisia amygdalina

Taj, Faqeer; Khan, Mubarak Ali; Ali, Huma; Khan, Raham Sher

doi:10.3390/plants8100430

Cited by 14 publications

(3 citation statements)

References 51 publications

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“…Xét tỷ lệ % mẫu tạo rễ giữa mẫu cấy mảnh lá và LMTB trong cùng điều kiện chiếu sáng không có sự khác biệt về mặt thống kê. Đến nay đã ghi nhận nhiều nghiên cứu tạo rễ bất định với kết quả tốt với các điều kiện chiếu sáng khác nhau như tốiví dụ trường hợp nuôi cấy mảnh lá Cichorium intybus (Nandagopal, Kumari, 2007), Centella asiatica (Ling et al, 2009a); chiếu sáng 15 µmol m -2 s -1 (1.111 lux) đối với Ophiorrhiza prostrata (Martin et al, 2008); 40 µmol m -2 s -1 (3.000 lux) đối với Artemisia amygdalina (Taj et al, 2019), và 4.000 -5.000 lux ở nuôi cấy tạo rễ từ mô lá Vernonia amygdalina (Khalafalla et al, 2009). Tương tự một số trường hợp nuôi cấy tạo rễ nêu trên, kết quả nghiên cứu này cho thấy mảnh lá/LMTB NGBCC tái sinh rễ bất định tốt ở các nghiệm thức có cường độ chiếu sáng 2.000 lux và 4.000 lux.…”

Section: ảNh Hưởng Của Cường độ Chiếu Sáng đếN Cảm ứNg Tạo Rễ Bất địNh Trực Tiếp Từ Mô Lá Ngbccunclassified

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Luy¹,

Ho²,

Lệ³

2021

Vietnam J. Biotechnol.

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Schefflera octophylla (Lour.) Harms is a precious plant species belonging to the Araliaceae family. All parts of plant have been used to create products for human health. In tissue culture of medicinal plants, the induction and multiplication of adventitious root of Schefflera octophylla for biomass collection have been studied. In this report, results on induction of adventitious root from leaf explants cultured in vitro of this plant species were presented. Leaf disks (~ 10 x 10 mm), leaf transverse - thin cell layers (t-TCLs) (~ 3 x 10 mm) were cultured on different mineral media MS, ½MS, B5, SH with NAA (0 - 5 mg/L), sucrose (0 - 50 g/L) and light intensity (0 - 4,000 lux). The results showed that, 30 days after culturing on ½MS solid medium plus 3 mg/L NAA, and 30 g/L sucrose in 4,000 lux light condition, direct formation of adventitious root was best from leaf disks, t-TCLs with rooting rate (%) 100, 100; root number/sample 68.80, 21.96; root lenght (mm) 16.53, 15.53, respectively. Leaf disk culture resulted in better rooting than t-TCL culture in two criteria of root number and root length. Morphological and histological observations of adventitious root primordia formation in the leaf disk were also performed. This is the first report on direct formation of adventitious root by in vitro culture of leaf disks/t-TCLs in Schefflera octophylla with very high efficiency, creating basis for further studies on root biomass multiplication for production of bioactive compounds.

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Section: ảNh Hưởng Của Cường độ Chiếu Sáng đếN Cảm ứNg Tạo Rễ Bất địNh Trực Tiếp Từ Mô Lá Ngbccunclassified

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Luy¹,

Ho²,

Lệ³

2021

Vietnam J. Biotechnol.

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“…The plants produce secondary metabolites in trace concentrations; the synthesis, however, depends upon different factors, such as physiological conditions, growth stages and environmental situations [ 2 ]. Different classes of secondary metabolites (SMs), such as phenolic, flavonoids, alkaloids, triterpenoids, saponins and steroids, are of great human interest as these serve as potent therapeutic drugs in the pharmaceutical industry [ 3 ]. These plant secondary metabolites are now being extensively utilized in herbal, cosmetic and nutraceutical formulations [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

Mamgain,

Mujib,

Bansal

et al. 2023

Plants

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Tylophora indica (Burm. f.) Merrill is an endangered medicinal plant that possesses various active agents, such as tylophorinine, kaempferol, quercetin, α-amyrin and beta-sitosterol, with multiple medicinal benefits. α-amyrin, a triterpenoid, is widely known for its antimicrobial, anti-inflammatory, gastroprotective and hepatoprotective properties. In this study, we investigated the metabolite profiling of tissues and the effects of cadmium chloride and chitosan on in vitro accumulation of alkaloids in T. indica. First, the callus was induced from the leaf in 2,4-D-, NAA- and/or BAP-fortified MS medium. Subsequent shoot formation through organogenesis and in vitro roots was later induced. Gas chromatography–mass spectrometry (GC–MS)-based phytochemical profiling of methanolic extracts of in vivo and in vitro regenerated plants was conducted, revealing the presence of the important phytocompounds α-amyrin, lupeol, beta-sitosterol, septicine, tocopherol and several others. Different in vitro grown tissues, like callus, leaf and root, were elicited with cadmium chloride (0.1–0.4 mg L−1) and chitosan (1–50 mg L−1) to evaluate the effect of elicitation on α-amyrin accumulation, measured with high-performance thin layer chromatography (HPTLC). CdCl2 and chitosan showed improved sugar (17.24 and 15.04 mg g−1 FW, respectively), protein (10.76 and 9.99 mg g−1 FW, respectively) and proline (7.46 and 7.12 mg g−1 FW), especially at T3 (0.3 and 25 mg L−1), in the leaf as compared to those of the control and other tissues. The antioxidant enzyme activities were also evaluated under an elicitated stress situation, wherein catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) displayed the highest activities in the leaf at T4 of both of the two elicitors. The α-amyrin yield was quantified with HPTLC in all tested tissues (leaf, callus and root) and had an Rf = 0.62 at 510 nm wavelength. Among all the concentrations tested, the T3 treatment (0.3 mg L−1 of cadmium chloride and 25 mg L−1 of chitosan) had the best influence on accumulation, irrespective of the tissues, with the maximum being in the leaf (2.72 and 2.64 μg g−1 DW, respectively), followed by the callus and root. Therefore, these results suggest future opportunities of elicitors in scaling up the production of important secondary metabolites to meet the requirements of the pharmaceutical industry.

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“…Taj et al, 2019). It had been proved to be a key factor affecting micrografting survival in Citrus(Navarro et al, 1975; Thimmappaiah et al, 2002).…”

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confidence: 99%

Establishment of a n efficient method for kiwifruit in vitro micrografting

Yao

Kong²,

Lei

et al. 2022

Preprint

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This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of kiwifruit ‘Miliang-1’ and ‘Hongyang’ was used as rootstock and scion. Accomplishment of in vitro grafting has been examined by varying numerous factors, the physiological state of the rootstock and scion, the pH value of the medium, the concentration of sucrose, the type of medium and the PGRs. The results showed that the best condition of kiwifruit in vitro grafting was that the rootstock does not take root, and both the rootstock and scion have no leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid media along with 0.5 mg L− 1 GA3, 1.0mg L− 1 IBA, 40 g L− 1 sucrose and 7 g L− 1 agar, pH 6.0. The matrix formula of perlite: peat soil: vermiculite volume ratio of 1:2:1 was the most suitable substrate for transplanting micrografted seedlings.

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Improved Production of Industrially Important Essential Oils Through Elicitation in the Adventitious Roots of Artemisia amygdalina

Cited by 14 publications

References 51 publications

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Direct induction of adventitious root in Schefflera octophylla (Lour.) Harms from leaf explants cultured in vitro

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

Establishment of a n efficient method for kiwifruit in vitro micrografting

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