Effects of low level laser therapy (LLLT) on pressured human osteoblasts: A histomorphologic and quantitative study

Pyo, Se-Jeong; Song, Won-Wook; Kim, I. R.; Park, B. S.; Kim, C. H.; Kim, Sung Sik; Chung, Il‐Kwun; Kim, Y. D.

doi:10.1134/s1054660x12030164

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…However, XPA mRNA expression is decreased in muscle tissue, suggesting a tissue-dependent effect (figure 2). Low-intensity laser radiation is considered to increase cellular metabolism [2][3][4][5][6][7][8][9][10], and this effect could be more effective on muscle tissue, explaining the lower XPA mRNA expression relative to GAPDH mRNA expression. In fact, absorption and scattering of laser light is different in each equivalent volumetric element in these tissues because of their different physical properties, such as refractive index [2], and radiation doses in muscle tissue could be lower than in skin tissue; this could explain, at least in part, the opposite results obtained in skin and muscle tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Frequency is extremely pure, and this characteristic is associated with their high intensity compared with other light sources and makes laser light useful for a number of biomedical applications [2]. Studies have reported and discussed the biostimulative effect of low-intensity red and near-infrared lasers in prokaryotic and eukaryotic cells [3][4][5][6][7][8][9][10][11][12]. This cellular effect is the reason why red and near-infrared low-intensity lasers in clinical protocols are used in a variety of health care settings for soft tissue repair (wound healing) and treatment of musculoskeletal pain and neurologic and inflammatory conditions.…”

Section: Introductionmentioning

confidence: 99%

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Low-intensity red and infrared lasers on XPA and XPC gene expression

Fonseca¹,

Magalhães²,

Mencalha³

et al. 2014

Laser Phys. Lett.

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Laser devices emit monochromatic, coherent, and highly collimated intense beams of light that are useful for a number of biomedical applications. However, for low-intensity lasers, possible adverse effects of laser light on DNA are still controversial. In this work, the expression of XPA and XPC genes in skin and muscle tissue exposed to low-intensity red and infrared lasers was evaluated. Skin and muscle tissue of Wistar rats were exposed to low-intensity red and infrared lasers at different fluences in continuous mode emission. Skin and muscle tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of actin gene expression by quantitative polymerase chain reaction. Data obtained show that laser radiation alters the expression of XPA and XPC mRNA differently in skin and muscle tissue of Wistar rats, depending on physical (fluence and wavelength) and biological (tissue) parameters. Laser light could modify expression of genes related to the nucleotide excision repair pathway at fluences and wavelengths used in clinical protocols.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Low-intensity red and infrared lasers on XPA and XPC gene expression

Fonseca¹,

Magalhães²,

Mencalha³

et al. 2014

Laser Phys. Lett.

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“…A number of studies have reported and discussed the biostimulative effect of low-intensity red and near infrared lasers on prokaryotic and eukaryotic cells [1][2][3][4][5][6][7][8][9][10][11]. This cellular effect is the reason why red and near infrared low-intensity lasers are used in low-level laser therapy protocols in a variety of health care settings for soft tissues repair (wound healing) and treatment of musculoskeletal pain, neurological and inflammatory conditions, as well as dental diseases.…”

Section: Introductionmentioning

confidence: 99%

Low-intensity infrared lasers alter actin gene expression in skin and muscle tissue

et al. 2012

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The biostimulative effect of low-intensity lasers is the basis for treatment of diseases in soft tissues. However, data about the influence of biostimulative lasers on gene expression are still scarce. The aim of this work was to evaluate the effects of low-intensity infrared lasers on the expression of actin mRNA in skin and muscle tissue. Skin and muscle tissue of Wistar rats was exposed to low-intensity infrared laser radiation at different fluences and frequencies. One and 24 hours after laser exposure, tissue samples were withdrawn for total RNA extraction, cDNA synthesis and evaluation of actin gene expression by quantitative polymerase chain reaction. The data obtained show that laser radiation alters the expression of actin mRNA differently in skin and muscle tissue of Wistar rats depending of the fluence, frequency and time after exposure. The results could be useful for laser dosimetry, as well as to justify the therapeutic protocols for treatment of diseases of skin and muscle tissues based on low-intensity infrared laser radiation.

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“…Another study reported that LIPUS induced BMP-2 to accelerate bone formation in animal models 69 . Note, however, that the inconsistent volume and the limitation of the effect of low-level laser in disadvantageous bone environment was reported in the in vitro test 60 . Still, LLLT also requires more additional studies for clinical application.…”

Section: Llltmentioning

confidence: 99%

“…For bone metabolism capacity in particular, stimulation by compression or function of osteoblast under a hypoxic environment was accelerated through laser irradiation; this becomes the basis 60 for clinical application in disadvantageous situations such as aging and post-surgical care.…”

Section: Lipusmentioning

confidence: 99%

Biophysical therapy and biostimulation in unfavorable bony circumstances: adjunctive therapies for osseointegration

Kim

2012

J Korean Assoc Oral Maxillofac Surg

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Dental implants using titanium have greatly advanced through the improvement of designs and surface treatments. Nonetheless, the anatomical limits and physiological changes of the patient are still regarded as obstacles in increasing the success rate of implants further, even with the enhancement of implant products. So there have been many efforts to overcome these limits. The intrinsic potential for bone regeneration can be stimulated through adjuvant treatments with the continuous improvement of implant properties, and this can play an important role in achieving optimum osseointegration toward peripheral bone tissue and securing ultimate long-term implant stability in standard surgical procedures. For this purpose, various chemical, biological, or biophysical measures were developed such as bone grafts, materials, pharmacological agents, growth factors, and bone formation proteins. The biophysical stimulation of bone union includes non-invasive and safe methods. In the beginning, it was developed as a method to enhance the healing of fractures, but later evolved into Pulsed Electromagnetic Field, Low-Intensity Pulsed Ultrasound, and Low-Level Laser Therapy. Their beneficial effects were confirmed in many studies. This study sought to examine bone-implant union and its latest trend as well as the biophysical stimulation method to enhance the union. In particular, this study suggested the enhancement of the function of cells and tissues under a disadvantageous bone metabolism environment through such adjunctive stimulation. This study is expected to serve as a treatment guideline for implant-bone union under unfavorable circumstances caused by systemic diseases hampering bone metabolism or the host environment.

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Effects of low level laser therapy (LLLT) on pressured human osteoblasts: A histomorphologic and quantitative study

Cited by 4 publications

References 31 publications

Low-intensity red and infrared lasers on XPA and XPC gene expression

Low-intensity red and infrared lasers on XPA and XPC gene expression

Low-intensity infrared lasers alter actin gene expression in skin and muscle tissue

Biophysical therapy and biostimulation in unfavorable bony circumstances: adjunctive therapies for osseointegration

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