Low-intensity red and infrared lasers on XPA and XPC gene expression

Fonseca, A S; Magalhães, Luís Alexandre Gonçalves; Mencalha, André Luiz; Ferreira-Machado, Samara Cristina; Geller, Mauro; Paoli, Flávia de

doi:10.1088/1612-2011/11/9/095601

Cited by 9 publications

(9 citation statements)

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“…Our results also emphasizing that mRNA expression from genes related to DNA repair systems can be differentially induced in skin and muscle tissues after exposure to lowintensity lasers [43]. There are few studies comparing laser effects on mRNA expression from genes related to DNA lesion repair in different tissues.…”

Section: Discussionsupporting

confidence: 50%

“…Previous data demonstrated that infrared laser exposure differently affects mRNA expression from genes coding enzymes involved in BER pathway of nuclear (APE1, OGG1, APEX1, and APEX2) [19,20,39] and mitochondrial (γ-polimerase) [20] DNA repair in skin and muscle tissue after infrared laser exposure. XPA and XPC mRNA expression is also differently altered in skin and muscle tissue after low-intensity infrared laser [43]. These findings could be explained due to different chromophores or different chromophore concentrations in skin and muscle cells.…”

Section: Discussionmentioning

confidence: 95%

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Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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Lasers emit light beams with specific characteristics, in which wavelength, frequency, power, fluence, and emission mode properties determine the photophysical, photochemical, and photobiological responses. Low-intensity lasers could induce free radical generation in biological tissues and cause alterations in macromolecules, such as DNA. Thus, the aim of this work was to evaluate excision repair cross-complementing group 1 (ERCC1) and excision repair cross-complementing group 2 (ERCC2) messenger RNA (mRNA) expression in biological tissues exposed to low-intensity lasers. Wistar rat (n = 28, 4 for each group) skin and muscle were exposed to low-intensity red (660 nm) and near-infrared (880 nm) lasers at different fluences (25, 50, and 100 J/cm(2)), and samples of these tissues were withdrawn for RNA extraction, cDNA synthesis, and gene expression evaluation by quantitative polymerase chain reaction. Laser exposure was in continuous wave and power of 100 mW. Data show that ERCC1 and ERCC2 mRNA expressions decrease in skin (p < 0.001) exposed to near-infrared laser, but increase in muscle tissue (p < 0.001). ERCC1 mRNA expression does not alter (p > 0.05), but ERCC2 mRNA expression decreases in skin (p < 0.001) and increases in muscle tissue (p < 0.001) exposed to red laser. Our results show that ERCC1 and ERCC2 mRNA expression is differently altered in skin and muscle tissue exposed to low-intensity lasers depending on wavelengths and fluences used in therapeutic protocols.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 95%

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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“…Notably, no telomere shortening was observed in healthy animals irradiated with a laser at 20 J cm −2 . A study on the evaluation of the influence of a low-power laser therapy on parameters of oxidative stress and DNA damage in skeletal muscle and plasma of rats with heart failure demonstrated that the DNA damage index had a significant increase at 21 J cm −2 compared to that at 3 J cm −2 , which suggests that high doses of a low-power laser seem to increase the DNA damage [ 41 ]. On the other hand, a study on the evaluation of DNA damage in blood cells of healthy animals after red and infrared laser irradiations demonstrated that the DNA damage is not dependent on the fluence because the irradiation at 50 J cm −2 induced higher levels of DNA damage in blood cells than those at 25 and 100 J cm −2 [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Low-power infrared laser modulates telomere length in heart tissue from an experimental model of acute lung injury

Trajano

Sergio

Oliveira

et al. 2021

Photochem Photobiol Sci

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Acute lung injury and acute respiratory distress syndrome can occur as a result of sepsis. Cardiac dysfunction is a serious component of multi-organ failure caused by severe sepsis. Telomere shortening is related to several heart diseases. Telomeres are associated with the shelterin protein complex, which contributes to the maintenance of telomere length. Low-power infrared lasers modulate mRNA levels of shelterin complex genes. This study aimed to evaluate effects of a low-power infrared laser on mRNA relative levels of genes involved in telomere stabilization and telomere length in heart tissue of an experimental model of acute lung injury caused by sepsis. Animals were divided into six groups, treated with intraperitoneal saline solution, saline solution and exposed to a low-power infrared laser at 10 J cm −2 and 20 J cm −2 , lipopolysaccharide (LPS), and LPS and, after 4 h, exposed to a low-power infrared laser at 10 J cm −2 and 20 J cm −2 . The laser exposure was performed only once. Analysis of mRNA relative levels and telomere length by RT-qPCR was performed. Telomere shortening and reduction in mRNA relative levels of TRF1 mRNA in heart tissues of LPS-induced ALI animals were observed. In addition, laser exposure increased the telomere length at 10 J cm −2 and modulated the TRF1 mRNA relative levels of at 20 J cm −2 in healthy animals. Although the telomeres were shortened and mRNA levels of TRF1 gene were increased in nontreated controls, the low-power infrared laser irradiation increased the telomere length at 10 J cm −2 in cardiac tissue of animals affected by LPS-induced acute lung injury, which suggests that telomere maintenance is a part of the photobiomodulation effect induced by infrared radiation.

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“…Although, biological effects caused by free radicals induced by low-level laser radiation are controversial, the possible adverse effects on cells and DNA damage after laser exposure have been reported in eukaryotic cells [13] and prokaryotic cells, at different powers, wavelengths, and fluences [12,14], as well as inducing adaptation or DNA damage at sub-lethal levels [14]. In addition, low-level lasers alter the relative expression of mRNA from DNA repair genes in tissues from Wistar rats [13,[15][16][17].…”

Section: Laser Physicsmentioning

confidence: 99%

Nuclear phenotype evaluation in skeletal muscle fromWistarrats exposed to low-level lasers

et al. 2017

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Low-level laser therapy includes devices emitting red and near-infrared radiation with output power below 100 mW. These devices are successfully used for the treatment of injuries and to improve exercise performance based on their biomodulatory effect. Despite the wide use of clinical protocols based on these lasers, the laser-induced effects on DNA are still disputed. Thus, the objective of this study was to investigate chromatin organization, ploidy degrees, and DNA fragmentation in skeletal muscle tissue from Wistar rats exposed to low-level red and infrared lasers. Wistar rats were exposed to low-level red and infrared lasers (25, 50, and 100 J cm −2 , 100 mW, continuous-wave emission mode) and, after 24h, samples of this tissue were withdrawn for the analysis of chromatin organization, ploidy degrees, and DNA fragmentation by Feulgen reaction detection of micronucleus, and apoptosis by TUNEL assay. Data obtained show that low-level red and infrared lasers alter geometric and densitometric parameters as well ploidy degree in muscle nuclei from Wistar rats, but do not induce DNA fragmentation, chromatin loss, and apoptosis at fluences taken out from clinical protocols.

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Low-intensity red and infrared lasers on XPA and XPC gene expression

Cited by 9 publications

References 58 publications

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

Low-power infrared laser modulates telomere length in heart tissue from an experimental model of acute lung injury

Nuclear phenotype evaluation in skeletal muscle fromWistarrats exposed to low-level lasers

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