Low-intensity infrared lasers alter actin gene expression in skin and muscle tissue

Fonseca, A S; Mencalha, André Luiz; Campos, Vera Maria Araújo de; Ferreira-Machado, Samara Cristina; Peregrino, Antônio Augusto de Freitas; Magalhães, Luís Alexandre Gonçalves; Geller, Mauro; Paoli, Flávia de

doi:10.1088/1054-660x/23/2/025602

Cited by 7 publications

(11 citation statements)

References 38 publications

(41 reference statements)

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“…Also, red laser has no effect on XPA mRNA expression in burned skin [18]. In addition, laser-induced alteration on gene expression has been reported to alter the expression of other genes, as type 1 collagen [40], actin [19], myogenin and VEGF [41], Cox-2 [42], as well as mRNA from genes related to BER pathway of DNA repair [19,20].…”

Section: Discussionmentioning

confidence: 99%

“…There are few studies comparing laser effects on mRNA expression from genes related to DNA lesion repair in different tissues. Previous data demonstrated that infrared laser exposure differently affects mRNA expression from genes coding enzymes involved in BER pathway of nuclear (APE1, OGG1, APEX1, and APEX2) [19,20,39] and mitochondrial (γ-polimerase) [20] DNA repair in skin and muscle tissue after infrared laser exposure. XPA and XPC mRNA expression is also differently altered in skin and muscle tissue after low-intensity infrared laser [43].…”

Section: Discussionmentioning

confidence: 99%

“…Base excision repair acts on single strand breaks, oxidized bases, and few bulky adducts [15,16], induced by endogenous free radicals or from exogenous exposure to genotoxic agents [17]. Previous data demonstrated that the expression of messenger RNA (mRNA) related to base excision repair is altered in biological tissues exposed to low-intensity red [18] and infrared [19,20] lasers. Also, exposure to low-intensity lasers alters the expression of mRNA related to nucleotide excision repair [18,20].…”

Section: Introductionmentioning

confidence: 99%

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Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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Lasers emit light beams with specific characteristics, in which wavelength, frequency, power, fluence, and emission mode properties determine the photophysical, photochemical, and photobiological responses. Low-intensity lasers could induce free radical generation in biological tissues and cause alterations in macromolecules, such as DNA. Thus, the aim of this work was to evaluate excision repair cross-complementing group 1 (ERCC1) and excision repair cross-complementing group 2 (ERCC2) messenger RNA (mRNA) expression in biological tissues exposed to low-intensity lasers. Wistar rat (n = 28, 4 for each group) skin and muscle were exposed to low-intensity red (660 nm) and near-infrared (880 nm) lasers at different fluences (25, 50, and 100 J/cm(2)), and samples of these tissues were withdrawn for RNA extraction, cDNA synthesis, and gene expression evaluation by quantitative polymerase chain reaction. Laser exposure was in continuous wave and power of 100 mW. Data show that ERCC1 and ERCC2 mRNA expressions decrease in skin (p < 0.001) exposed to near-infrared laser, but increase in muscle tissue (p < 0.001). ERCC1 mRNA expression does not alter (p > 0.05), but ERCC2 mRNA expression decreases in skin (p < 0.001) and increases in muscle tissue (p < 0.001) exposed to red laser. Our results show that ERCC1 and ERCC2 mRNA expression is differently altered in skin and muscle tissue exposed to low-intensity lasers depending on wavelengths and fluences used in therapeutic protocols.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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“…Laser exposure and zymosan-induced did cause neither DNA fragmentation nor cell death by caspase-6 pathway. Several studies have been reported controversial and possible adverse effects on cells and data about DNA damage after laser exposure in eukaryotic 42,43 and prokaryotic cells 32,[44][45] at different powers, wavelengths and fluences. In addition, as regard to DNA, a variety of studies have suggested that exposure to red and near infrared low-intensity lasers could induce adaptation or DNA damage at a sub-lethal level 32, 45 .…”

Section: Discussionmentioning

confidence: 99%

Low-intensity infrared laser effects on zymosan-induced articular inflammatory response

et al. 2015

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Low-level therapy laser is a phototherapy treatment that involves the application of low power light in the red or infrared wavelengths in various diseases such as arthritis. In this work, we investigated whether low-intensity infrared laser therapy could cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosaninduced articular inflammatory process. Inflammatory process was induced in C57BL/6 mouse by intra-articular injection of zymosan into rear tibio-tarsal joints. Thirty animals were divided in five groups: (I) control, (II) laser, (III) zymosan-induced, (IV) zymosan-induced + laser and (V). Laser exposure was performed after zymosan administration with low-intensity infrared laser (830 nm), power 10 mW, fluence 3.0 J/cm 2 at continuous mode emission, in five doses. Twenty-four hours after last irradiation, the animals were sacrificed and the right joints fixed and demineralized. Morphological analysis was observed by hematoxylin and eosin stain, pro-apoptotic (caspase-6) was analyzed by immunocytochemistry and DNA fragmentation was performed by TUNEL assay in articular cartilage cells. Inflammatory process was observed in connective tissue near to articular cartilage, in IV and V groups, indicating zymosan effect. This process was decreased in both groups after laser treatment and dexamethasone. Although groups III and IV presented higher caspase-6 and DNA fragmentation percentages, statistical differences were not observed when compared to groups I and II. Our results suggest that therapies based on low-intensity infrared lasers could reduce inflammatory process and could not cause death by caspase-6 apoptosis or DNA damage pathways in cartilage cells after zymosan-induced articular inflammatory process.

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“…Biological effects caused by free radicals from laser light are controversial and possible adverse effects on cells and data about DNA damage after laser exposure have been reported in eukaryotic [10][11][12][13][14] and prokaryotic cells [15][16][17][18][19][20][21][22][23] , at different powers, wavelengths and fluences. In addition, as regard to DNA, a variety of studies have suggested that exposure to red and near infrared low-intensity lasers could induce adaptation or DNA damage at a sub-lethal level.…”

Section: Introductionmentioning

confidence: 99%

DNA fragmentation and nuclear phenotype in tendons exposed to low-intensity infrared laser

et al. 2015

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Clinical protocols are recommended in device guidelines outlined for treating many diseases on empirical basis. However, effects of low-intensity infrared lasers at fluences used in clinical protocols on DNA are controversial. Excitation of endogenous chromophores in tissues and free radicals generation could be described as a consequence of laser used. DNA lesions induced by free radicals cause changes in DNA structure, chromatin organization, ploidy degrees and cell death. In this work, we investigated whether low-intensity infrared laser therapy could alter the fibroblasts nuclei characteristics and induce DNA fragmentation. Tendons of Wistar rats were exposed to low-intensity infrared laser (830 nm), at different fluences (1, 5 and 10 J/cm 2 ), in continuous wave (power output of 10mW, power density of 79.6 mW/cm 2 ). Different frequencies were analyzed for the higher fluence (10 J/cm 2 ), at pulsed emission mode (2.5, 250 and 2500 Hz), with the laser source at surface of skin. Geometric, densitometric and textural parameters obtained for Feulgen-stained nuclei by image analysis were used to define nuclear phenotypes. Significant differences were observed on the nuclear phenotype of tendons after exposure to laser, as well as, high cell death percentages was observed for all fluences and frequencies analyzed here, exception 1 J/cm 2 fluence. Our results indicate that low-intensity infrared laser can alter geometric, densitometric and textural parameters in tendon fibroblasts nuclei. Laser can also induce DNA fragmentation, chromatin lost and consequently cell death, using fluences, frequencies and emission modes took out from clinical protocols.

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Low-intensity infrared lasers alter actin gene expression in skin and muscle tissue

Cited by 7 publications

References 38 publications

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

Low-intensity infrared laser effects on zymosan-induced articular inflammatory response

DNA fragmentation and nuclear phenotype in tendons exposed to low-intensity infrared laser

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