Effects of lipidic carbon sources on the extracellular lipolytic activity of a newly isolated strain of Bacillus subtilis

Abstract

An isolate exhibiting high extracellular lipolytic activity was identified as Bacillus subtilis by 16S rRNA gene sequence analysis. The enzyme activity of the isolate was improved by using different concentrations of lipidic carbon sources such as vegetable oils, fatty acids and triglycerides. Lipolytic activity was assayed spectrophotometrically using p-nitrophenyl palmitate. One percent (v/v) of sesame oil provided the highest activity with 80 and 98% enhancements with respect to 1% (v/v) concentrations of linoleic acid and triolein as the favored fatty acid and triglyceride, respectively. Glucose presented a repressive effect on lipase production. Lipase secreted by B. subtilis was partially purified by ultrafiltration and anion exchange chromatography; and the purified enzyme was tested for its residual activity in the presence of EDTA, SDS, Triton X-100, Tween 20, Tween 80 and protease. The present work reports, for the first time, that the lipolytic activity of a B. subtilis strain can be improved by using inexpensive vegetable oils; and also that B. subtilis lipase is suitable for use in detergents.

A Review on Microbial Lipases Production

“…Glucose, olive oil, peptone, and FeCl 3 ⋅6H 2 O were found to have more significant influence on lipase production by C. rugosa. Takaç and Marul (2008) improved the lipase production by B. subtilis using different concentrations of lipidic carbon sources such as vegetable oils, fatty acids, and triglycerides. One percent of sesame oil afforded the highest activity with 80% and 98% enhancements with respect to 1% concentrations of linoleic acid and triolein as the favored fatty acid and triglyceride, respectively.…”

“…The genes that encode lipase in Candida sp., Geotrichum sp., Trichosporon sp., and Y. lipolytica have been cloned and Bacterial Takaç and Marul (2008) over-expressed (Wang et al 2007). Although lipases from C. rugosa and C. antarctica have been extensively used in different fields, there are several recent publications reporting the production of lipases by other yeasts, as shown in Table 1.…”

“…Abada (2008) produced lipase from a strain of B. stearothermophilus AB-1 isolated from air and obtained a maximum lipase activity of 1,585 U mL −1 in 48 h of fermentation. Takaç and Marul (2008) isolated microbial cultures from soil enriched by periodic sub-culturing of samples in nutrient broth containing 1% (v/v) tributyrin. The isolation process was performed by serial dilution samples on tributyrin agar (TBA) plates.…”

Characterization of an extracellular thermophilic alkaline esterase produced by Bacillus subtilis DR8806

“…The fluorescent halo zone surrounding colony was used as an indicator of lipolytic activity [16,17]. The optimum temperature of the growth was determined by culturing the B. subtilis DR8806 in a medium containing 0.5% (w/v) peptone, 0.5% (w/v) yeast extracts, and 3.0% (v/v) olive oil [18]. The medium was incubated under shaking (120 rpm) at 37, 42 and 50 • C for 34 h. The samples were withdrawn at two-hour intervals.…”

Production of multifunctional lipases by Penicillium verrucosum and Penicillium brevicompactum under solid state fermentation of babassu cake and castor meal

“…Takaç and Marul [36] showed that the time necessary to reach the maximum activity is dependent on the type of vegetable oil used as supplement. However, an enhancement in fermentation time leads to a drop of lipase activity, independent on the vegetable oil used.…”