Effects of light intensity and addition of carotene rich Dunaliella salina live cells on growth and antioxidant activity of Solea senegalensis Kaup (1858) larval and metamorphic stages

Abstract

Senegal sole Solea senegalensis larval and metamorphic stages were exposed to a range of light intensities (200, 1000 and 2000 lx) in cultures with or without supplementation of β‐carotene‐rich live Dunaliella salina cells. Antioxidant biomarkers such as superoxide dismutase (SOD), catalase (KAT), total glutathione peroxidase (t‐GPX) and malondialdehyde (MDA) were determined in larval and metamorphic stages. Growth was not affected (P > 0·05) either by light intensity or D. salina supplementation. Survival after metamorphosis was also unaffected by D. salina supplementation (mean ±s.e. 81·0 ± 2·5% against 80·6 ± 2·9% those fed the control algal diet) or light intensity (mean ±s.e. 74·3 ± 4·9% for 200 lx, 85·1 ± 2·7% for 1000 lx and 82·8 ± 5·2% for 2000 lx, respectively). Light intensity affected (P < 0·05) KAT and t‐GPX throughout development. SOD was only affected in metamorphosing larvae. The highest KAT and t‐GPX activities were detected when the lowest light intensity (200 lx) was used. Light had no effect (P > 0·05) on MDA at any stage. Supplementing the diet with D. salina did not affect SOD, KAT or t‐GPX and there was no interaction (P > 0·05) with light intensity. MDA was the only biomarker whose activity was significantly (P < 0·05) reduced when D. salina was supplemented to the larval rearing tanks. The effect of D. salina supplementation was only detected in metamorphosing larvae, whose MDA levels were noticeably higher than in earlier stages. These results are evidence of the antiperoxidative effect of β‐carotene from live algae in the larval rearing process of marine fishes.

Effects of light intensity on growth, feeding activity and development in common sole (Solea soleaL.)larvae in relation to sensory organ ontogeny

“…On the other hand, our results suggest that an increment of light intensity from 50 to 1000 lx does not bring about any improvement in prey ingestion, WW or SGR at 12 dph. This last finding was also observed in Senegal sole larvae (Cañavate et al 2007), where different light intensities, in the range of 200 to 2000 lx, had no effect on growth from 3 to 20 dph.…”

“…This last finding was also observed in Senegal sole larvae (Cañavate et al . ), where different light intensities, in the range of 200 to 2000 lx, had no effect on growth from 3 to 20 dph.…”

“…We thus investigated the effects of light intensity on growth, feeding activity and development in common sole larvae with the aim of providing useful information for hatcheries. To this end, common sole larvae were exposed to four different light intensity regimes, from 3 to 1000 lux, following previous studies on other marine flatfish (Huse 1994;Henne & Watanabe 2003;Cañavate, Prieto, Zerolo, Sole, Sarasquete & Fern andez-D ıaz 2007). The trial lasted 48 days, covering the entire sole larval cycle, from before to after metamorphosis.…”

Effect of replacement of fish meal protein with boiled full fat soybean seeds and dried algae on growth performance, nutrient utilization and some blood parameters of Nile tilapia (Oreochromis niloticus)

“…The author also added that, lipid content was significantly higher (P < 0.05) in fish fed diets in which SBF substituted HFM for 50% or more and hematological values of test fishes were within normal values, which were also proved in the current study (Table 10). Cañavate et al,(2007) reported that growth of Senegal sole larval and metamorphic stages was not affected by supplementing the diet with D. salina as a dried algae and did not affect antioxidant biomarkers; malondialdehyde (MDA) which was the only biomarker whose activity was significantly reduced when D. salina was supplemented to the larval rearing tanks. These results are evidence of the antiperoxidative effect of β-carotene from algae in the larval rearing process of fishes.…”

Short-term dietary supplementation with the microalgaParietochloris incisaenhances stress resistance in guppiesPoecilia reticulata

“…Microalgae‐based diets affected the immunocompetence of guppies in a field experiment where the load of the ectoparasite Gyrodactylus turnbulli was significantly lower in fish raised on a diet containing an intermediate level of carotenoids as compared with the trace‐ and high‐carotenoid‐fed groups (Kolluru et al 2006). Dietary supplementation of β‐carotene‐rich D. salina reduced lipid peroxidation in larvae of Solea senegalensis (Canavate, Prieto, Zerolo, Sole, Sarasquete & Fernandez‐Diaz 2007).…”