“…As shown in Figure 1 A, by calculating the duration between the key time points, several time-related parameters, including rise time, decay time, peak to end, and tail duration, as well as the whole wavelength, peak space, can be derived. These parameters were commonly used in previous studies on calcium transient analysis ( Yang et al., 2016 ; Yu et al., 2012 ). We also calculated peak widths at different prominences, including 10% (PW10), 25% (PW25), 50% (PW50), 80% (PW80), and 90% (PW90).…”