Effects of kefir supernatant and lactic acid bacteria isolated from kefir grain on cytokine production by macrophage

Hong, Wei-Sheng; Chen, Hsi-Chia; Chen, Yen‐Po; Chen, Ming‐Ju

doi:10.1016/j.idairyj.2008.10.010

Cited by 68 publications

(63 citation statements)

References 55 publications

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“…Komponen dinding sel dan sitoplasma beberapa jenis bakteri asam laktat menunjukkan efek sebagai imunostimulan (Hong et al, 2008). Pada penelitian lainnya, kefir mampu memodulasi sistem imun mukosa bergantung dengan dosis (Farnworth, 2005) dan memodulasi respons imun pada kultur PBMC (peripheral blood mononuclear cell) pasien tuberkulosis paru dewasa (Raras et al, 2015).…”

Section: Pendahuluanunclassified

“…Beberapa studi telah dilakukan dengan hewan model mengenai kefir yang memiliki efek sebagai imunomodulator yang membuktikan bahwa kefir merangsang sekresi sitokin sel Th1, termasuk IL-2, yang diketahui berperan dalam melawan bakteri patogen melalui TLR-2 pathway (Hong et al, 2008). Namun demikian belum ada penelitian yang menggunakan isolasi PBMC dari sukarelawan sehat untuk membuktikan efek supernatan kefir dari susu kambing terhadap sistem imun.…”

Section: Pembahasanunclassified

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The effect of Kefir on The Immune Response of Healthy Volunteers In Vitro

Wisudanti¹

2017

AMS

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Kefir merupakan bahan pangan fungsional probiotik, terbuat dari susu yang difermentasi dengan kefir grains yang mengandung berbagai jenis bakteri dan ragi yang bermanfaat. Telah banyak dilakukan penelitian tentang efek kefir per oral pada sistem kekebalan tubuh, tetapi sedikit penelitian yang membuktikan efek komponen bioaktif kefir yaitu peptida dan eksopolisakarida (kefiran), terhadap respons imun. Tujuan penelitian ini adalah untuk membuktikan efek supernatan kefir susu kambing terhadap respons imun sukarelawan sehat secara in vitro. Penelitian dilakukan pada 15 sukarelawan sehat, yang diisolasi PBMC-nya dari whole blood, kemudian dibagi menjadi 5 kelompok (K-, P1, P2, P3 dan P4) sebelum dilakukan kultur selama 4 hari. Hasil kultur diperiksa persentase sel T CD4 + , sel T CD8 + , IFN-γ, IL-4 menggunakan flowsitometri dan kadar IL-2, IL-10 menggunakan metode ELISA. Hasilnya didapatkan kefir tidak mempengaruhi persentase sel T CD4 + dan Sel T CD8+. Semakin tinggi konsentrasi kefir yang diberikan, maka semakin tinggi kadar IFN-γ dan IL-4 yang disekresikan, namun terjadi penurunan pada kadar IL-2. Peningaktan signifikan terjadi pada kadar IL-10 kultur PBMC yang diberikan kefir dengan berbagai konsentrasi (p<0,01), terutama pada konsentrasi 1%. Hasil tersebut juga menunjukkan efek penting komponen bioaktif kefir terhadap respons imun. Kesimpulan dari penelitian ini adalah kefir dapat meningkatkan respons imun, melalui stimulasi sekresi IL-10 secara in vitro.

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Section: Pendahuluanunclassified

Section: Pembahasanunclassified

The effect of Kefir on The Immune Response of Healthy Volunteers In Vitro

Wisudanti¹

2017

AMS

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“…The results indicated that the live bacteria, cell wall and DNA of the two lactobacilli exhibited immunomodulating activity. It was reported that some strains of Lactobacillus could enhance host immunity by stimulating immune cells to produce cytokine, such as IL-12 (p70), IFN-c and TNF-a, and exerting proliferative effect on T and B lymphocyte (Miettinen et al 1998;Kato et al 1999;Hong et al 2009). …”

Section: Effects Of Lactobacilli and Their Cell Components On Nk Cellmentioning

confidence: 99%

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk

Tuo

Zhang

Han

et al. 2010

World J Microbiol Biotechnol

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The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus coryniformis subsp torquens T3L (L. coryniformis T3L) isolated from traditional fermented yak's milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA of the two Lactobacillus strains on human peripheral blood mononuclear cells (PBMCs) proliferation, production of interleukin-12 (IL-12 p70), interferon gamma (IFN-c) and tumor necrosis factor alpha (TNF-a), and natural killer (NK) cell activity were assessed. The live bacteria, cell wall and genomic DNA of the two lactobacilli exerted proliferative effects on PBMCs. Live bacteria at 1 9 10 6 c.f.u. ml -1 , cell wall at 20 lg protein ml -1 and DNA at 50 lg DNA ml -1 of the strainS induced the secretion of IL-12 (p70), IFN-c and TNF-a by PBMCs. NK cell activities increased after cultivation of PBMCs with live bacteria, cell wall and DNA of the strains. Overall, these results demonstrate that the live bacteria, cell wall and genomic DNA of the two Lactobacillus strains exhibit immunomodulating activity.

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“…The product has been used for many years in Russia for the treatment of various types of illnesses. The functional properties attributed to kefir include retardation of cancerous tumors, (Murofushi et al 1983;Hosono et al 1990;Hong et al 2009), activation of the immunological system (Osada et al 1994;Vinderola et al 2005), possibility of consumption in cases of lactointolerance, combating of intestinal infections, and antagonistic activity toward pathogenic microorganisms (Hong et al 2009), among others (Zubillaga 2001). Due to its microbiotic constituent, several different substances, which inhibit the growth of pathogenic microorganisms are produced, which are frequently associated with the illnesses of dietary origin (Magalhães et al 2010;Tas et al 2012).…”

Section: Introductionmentioning

confidence: 99%