Effects of ionizing radiation on extracellular matrix

Mohamed, Faizal K.P. Kunchi; Bradley, D.A.; Winlove, C. Peter

doi:10.1016/j.nima.2007.05.236

Cited by 16 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The principle observation is fibre breakage associated with attack by free radicals, which results in skin deformation and loss of elasticity. Experiments on purified elastin exposed acutely to ionizing radiation have shown a decrease in elastic modulus [51]. This is consistent with fibre breakage in contrast to the cross-linking which occurs in collagen fibres.…”

Section: Mechanical Changes In Ageing and Diseasesupporting

confidence: 62%

The structure and micromechanics of elastic tissue

et al. 2014

View full text Add to dashboard Cite

Elastin is a major component of tissues such as lung and blood vessels, and endows them with the long-range elasticity necessary for their physiological functions. Recent research has revealed the complexity of these elastin structures and drawn attention to the existence of extensive networks of fine elastin fibres in tissues such as articular cartilage and the intervertebral disc. Nonlinear microscopy, allowing the visualization of these structures in living tissues, is informing analysis of their mechanical properties. Elastic fibres are complex in composition and structure containing, in addition to elastin, an array of microfibrillar proteins, principally fibrillin. Raman microspectrometry and X-ray scattering have provided new insights into the mechanisms of elasticity of the individual component proteins at the molecular and fibrillar levels, but more remains to be done in understanding their mechanical interactions in composite matrices. Elastic tissue is one of the most stable components of the extracellular matrix, but impaired mechanical function is associated with ageing and diseases such as atherosclerosis and diabetes. Efforts to understand these associations through studying the effects of processes such as calcium and lipid binding and glycation on the mechanical properties of elastin preparations in vitro have produced a confusing picture, and further efforts are required to determine the molecular basis of such effects.

show abstract

Section: Mechanical Changes In Ageing and Diseasesupporting

confidence: 62%

The structure and micromechanics of elastic tissue

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Ionizing radiation is known to lower proteoglycan content and compressive stiffness [8,[22][23][24][25]. Previous studies display that reactive oxygen radicals induced by ionizing radiation may be responsible for the degradation of the physiologically important glycosaminoglycan hyaluronan [10,26,27]. Degradation of the proteoglycans and type II collagen present within the matrix of articular cartilage relates to mechanisms of various enzymes [2].…”

Section: Resultsmentioning

confidence: 99%

“…There is not much data regarding effects of radiation on articular cartilage in the literature, although joint damage has been reported following the cancer treatment or occupational exposures [8]. Most studies on effects of radiation on tissue have concentrated on cells, with less attention paid to the extracellular matrix [10]. It has been found that the radiation causes an active degradation of cartilage, through the decline in proteoglycan synthesis, in the human and * e-mail: ekrcicek@gmail.com pig chondrocytes [8].…”

Section: Introductionmentioning

confidence: 99%

Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Çiçek

2016

Acta Phys. Pol. A

View full text Add to dashboard Cite

A load bearing tissue found at the ends of articulating bones, the articular cartilage provides low friction surfaces for efficient movement. Its mechanical properties are determined by the structure and composition of type II collagen, proteoglycans and interstitial fluid. This work investigates the effects of X-ray irradiation, previously shown to affect the biological properties of the articular tissue, on mechanical properties of articular cartilage using polarized light microscope for imaging and compressive modulus test applied to articular cartilage to determine the effects of the ionizing radiation on mechanical properties. According to the test results, the relaxation time is significantly longer in control than X-ray exposed samples, while the force is much higher showing that the X-ray irradiation causes the reduction in the stiffness of articular cartilage.

show abstract

“…As illustrated in Figure 4 and Figure 5 , GNPs still penetrated through the extracellular matrix (ECM). The ECM is an essential component of tissues that consists of collagen and elastin fibers immersed in visoelastic gel that is composed mainly of hyaluronan and proteoglycan [ 44 ]. This matrix of collagen networks prevents penetration of macromolecules and the delivery of therapeutic agents to cancer cells [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Yang

Bromma

Chithrani

2018

Cancers

View full text Add to dashboard Cite

Optimizing the interface between nanoparticles (NPs) and the biological environment at various levels should be considered for improving delivery of NPs to the target tumor area. For NPs to be successfully delivered to cancer cells, NPs needs to be functionalized for circulation through the blood vessels. In this study, accumulation of Polyethylene Glycol (PEG) functionalized gold nanoparticles (GNPs) was first tested using in vitro monolayer cells and multilayer cell models prior to in vivo models. A diameter of 10 nm sized GNP was selected for this study for sufficient penetration through tumor tissue. The surfaces of the GNPs were modified with PEG molecules, to improve circulation time by reducing non-specific uptake by the reticuloendothelial system (RES) in animal models, and with a peptide containing integrin binding domain, RGD (arginyl-glycyl-aspartic acid), to improve internalization at the cellular level. A 10–12% accumulation of the injected GNP dose within the tumor was observed in vivo and the GNPs remained within the tumor tissue up to 72 h. This study suggests an in vitro platform for optimizing the accumulation of NP complexes in cells and tissue structures before testing them in animal models. Higher accumulation within the tumor in vivo upon surface modification is a promising outcome for future applications where GNPs can be used for drug delivery and radiation therapy.

show abstract

Effects of ionizing radiation on extracellular matrix

Cited by 16 publications

References 11 publications

The structure and micromechanics of elastic tissue

The structure and micromechanics of elastic tissue

Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Contact Info

Product

Resources

About