Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Çiçek, Ekrem

doi:10.12693/aphyspola.129.200

Cited by 11 publications

(11 citation statements)

References 30 publications

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“…In instantaneous experiments however, although responses are similar to the control, it is evidently seen that increasing stress values match with lower strain curves. In a previous study on the effects of ROS resulted by x-ray radiation on equilibrium stiffness of cartilage matrix [6], experimental curves were found to be more similar to those of the control indicating higher deteriorating rate of direct effect of ROS on the mechanical properties of the articular cartilage.…”

Section: Discussionmentioning

confidence: 82%

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Hydrogen peroxide induced oxidative damage on mechanical properties of the articular cartilage

Çiçek

2017

Acta Biologica Hungarica

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Articular cartilage has unique mechanical and physicochemical properties which are responsible for its load carrying capabilities. This work investigates the effects of hydrogen peroxide induced oxidative damage on mechanical properties of articular cartilage. Bovine articular cartilage was exposed to hydrogen peroxide for a week. Dynamic and static mechanical tests applied to calculate articular cartilage compressive modulus. We observed higher control curve slopes than that of hydrogen peroxide curves which account for lesser stiffness values in the exposed articular cartilage. For the instantaneous experiments, results were statistically significant (p = 0.01, p < 0.05). Here report that hydrogen peroxide induced oxidative damage causes reduction in the stiffness of the articular cartilage.

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Section: Discussionmentioning

confidence: 82%

“…Initially, the force was applied and the cartilage was allowed to come to equilibrium. The cartilage surface was then displaced by 0.7 mm in total of ten steps; each step being 70 µm [6]. Stress was calculated as load per unit macroscopic sectional field.…”

Section: Compression Tests For Equilibrium Modulusmentioning

confidence: 99%

Hydrogen peroxide induced oxidative damage on mechanical properties of the articular cartilage

Çiçek

2017

Acta Biologica Hungarica

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“…Attempts were made to keep the overall scanning time similar for all samples but differences in exposure time and number of projections may still have affected comparison between the scans. Previous studies have shown that ionizing radiation can have a significant effect on a range of measured properties in articular cartilage samples 43,44 , including its mechanical properties 45 ; bone is also negatively affected 46 . Low energy X-rays interact with these low-density materials and cause more damage than high energy beams causing particular problems for the low voltages used throughout this study 47 .…”

Section: Discussionmentioning

confidence: 99%

Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level

Clark

Garbout

Ferreira

et al. 2020

Osteoarthritis and Cartilage

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Objective: High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular cartilage. Design: Bovine osteochondral plugs were prepared four ways: in phosphate-buffered saline (PBS) or 70% ethanol (EtOH), both with or without phosphotungstic acid (PTA) staining. Specimens were imaged with micro-CT following two protocols: 1) absorption contrast (AC) imaging 2) propagation phase-contrast (PPC) imaging. All samples were scanned in liquid. The contrast to noise ratio (C/N) of cellular features quantified scan quality and were statistically analysed. Cellular features resolved by micro-CT were validated by standard histology. Results: The highest quality images were obtained using propagation phase-contrast imaging and PTAstaining in 70% EtOH. Cellular features were also visualised when stained in PBS and unstained in EtOH. Under all conditions PPC resulted in greater contrast than AC (p < 0.0001 to p ¼ 0.038). Simultaneous imaging of cartilage and subchondral bone did not impede image quality. Corresponding features were located in both histology and micro-CT and followed the same distribution with similar density and roundness values. Conclusions: Three-dimensional visualisation and quantification of the chondrocyte population within articular cartilage can be achieved across a field of view of several millimetres using laboratory-based micro-CT. The ability to map chondrocytes in 3D opens possibilities for research in fields from skeletal development through to medical device design and treatment of cartilage degeneration.

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“…Six control samples, seven samples exposed to 3.33-GBq technetium-99m, and nine samples exposed to 2.035-GBq technetium-99m sestamibi were analysed. The details related to the compression method can be found in the previous study [15].…”

Section: Compression Tests For Equilibrium Modulusmentioning

confidence: 99%

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

Kuzu

Çiçek

2019

Nukleonika

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As radiation science and technology advances, nuclear medicine applications are increasing worldwide which necessitate the understanding of biological implications of such practices. Ionizing radiation has been shown to cause degraded matrix and reduced proteoglycan synthesis in cartilage, and the late consequences of which may include degenerative arthritis or arthropathy. Although degenerative effects of the ionizing radiation on cartilage tissue have been demonstrated, the effects on the mechanical properties of articular cartilage are largely unknown. The radiopharmaceuticals, technetium-99m and technetium-99m sestamibi, were utilized on bovine articular cartilage to investigate these effects. We used two different mechanical tests to determine the mechanical properties of articular cartilage. Dynamic and static mechanical tests were applied to calculate compressive modulus for articular cartilage. We observed clearly higher control modulus values than that of experimental groups which account for lesser stiffness in the exposed cartilage. In conclusion, compressive moduli of bovine articular cartilage were found to decrease after radiopharmaceutical exposure, after both instantaneous and equilibrium mechanical experiments.

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Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Cited by 11 publications

References 30 publications

Hydrogen peroxide induced oxidative damage on mechanical properties of the articular cartilage

Hydrogen peroxide induced oxidative damage on mechanical properties of the articular cartilage

Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level

Effects of radiopharmaceuticals on articular cartilage’s mechanical properties

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