Effects of intrauterine infusion of povidone-iodine on endometrial cytology and bacteriology in dairy cows with clinical endometritis

Abstract

Endometritis is one of the major diseases causing infertility in the cow. Intrauterine infusion of povidone-iodine (PVP-I) is a common treatment. However, the optimal concentration of PVP-I for treating endometritis effectively remains unknown. We tested concentrations of 2.0% or 0.5% PVP-I for treating clinical endometritis in dairy cattle. In Experiment 1, bacteria isolated from the uterus were incubated with either 2.0% or 0.5% PVP-I, and the numbers of bacterial colonies were counted. In Experiment 2, 18 cows with clinical endometritis were treated with either 2.0% or 0.5% PVP-I (n=9 in each group). Cytology samples and bacteria were collected using a cytobrush on weeks 0 (W0), 1 (W1) and 2 (W2) after treatment. Subsequent reproductive performance was compared between the two groups. In Experiment 1, both concentrations had a similar antiseptic outcome. In Experiment 2, the percentage of polymorphonuclear neutrophils (PMN%) in the endometrial epithelium at W2 in the 2.0% group was significantly lower (P<0.05) than in the 0.5% group, although the PMN% decreased significantly from W0 to W2 (P<0.01) in both groups. Decreases in bacterial infection rates from W0 to W2 were similar in both groups. The first service conception rate was higher, numbers of services per conception were fewer, and time to conception was shorter in the 2.0% group than in the 0.5% group. Thus, an intrauterine infusion of 2.0% PVP-I was better than 0.5% in treating clinical endometritis in these dairy cattle.

Effect of diagnosis and treatment of clinical endometritis based on vaginal discharge score grading system in postpartum Holstein cows

“…This is because >5% PMNs generally indicates impaired reproductive performance [ 1 ]. In addition, a decrease in bacterial infection and the percentage of PMNs is reflected in a reduction of the VDS [ 13 ]. Although cytology is a very useful method for diagnosing endometritis, this method is less suitable for clinical practice because it is more costly and time consuming.…”

“…The prevalence of clinical endometritis in dairy cows 15 to 60 days postpartum ranges from 23.6 to 42.6% [ 3 , 4 , 10 , 16 ]. Although different treatment methods have been utilized for endometritis, valid diagnostic criteria and optimal treatment timing have not been elucidated [ 2 , 7 , 8 , 12 , 13 , 22 , 23 ]. Rectal palpation of the genital tract is the most commonly used clinical method to detect uterine abnormalities due to its simplicity and rapid implementation in the field of veterinary practice [ 15 ].…”

“…A vaginoscope, with the aid of a flashlight, is used to visually detect the presence and quality of vaginal discharge [ 10 ]. A 4-point scoring system, the vaginal discharge score (VDS), has been used to classify the nature of the vaginal discharge [ 10 , 13 , 20 , 23 ]. Although the VDS grading system is well-known as a useful method to diagnose clinical endometritis and to estimate reproductive prognosis [ 3 , 4 , 20 ], information regarding the effectiveness of treatment for clinical endometritis using a VDS grading system under field conditions is lacking and controversial.…”

The Effectiveness of Lugol for Endometritis Therapy in Aceh Cow

“…Various therapies in cows with endometritis have been carried out using antibiotics, prostaglandins [5,6,7], and Lugol [8,9,10]. The use of Lugol 1% can treat reproductive disorders of cattle like uterus infection [11].…”

Intrauterine infusion of povidone-iodine: Its effect on the endometrium and subsequent fertility in postpartum dairy cows

“…To evaluate changes in the degree of endometrial inflammation, PMN% was measured every 24 hr for 8 days. A previous study showed that intrauterine infusion of PVP-I decreased PMN% within two weeks in cows with endometritis [ 18 ]. In the present study, the PVP group had transient inflammation the day after infusion that was reduced after 48 hr.…”