Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

Nakashima, Kazuki; Ishida, Aiko; Shimamoto, Saki; Ijiri, Daichi; Ohtsuka, Akira

doi:10.2141/jpsa.0160141

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Although it is well known that insulin and IGF-I have a regulatory effect on the expression of atrogin-1/MAFbx in mammalian skeletal muscle, their effects in chicken muscle cells are largely unknown. We previously reported that IGF-I suppresses the expression of atrogin-1/MAFbx in chick myotubes (Nakashima et al, 2017). In the present study, insulin suppressed the mRNA expression of atrogin-1/ MAFbx in chick myotubes.…”

Section: Resultssupporting

confidence: 62%

“…Myoblasts were isolated from the thigh muscles of 13-dayold chick embryos (Nakashima et al, 2017). Briefly, the muscle tissue obtained from the embryo was digested with dispase (Gibco, Waltham, MA USA), and the cell suspension was transferred to an uncoated culture dish to allow fibroblast attachment.…”

Section: Cell Culturementioning

confidence: 99%

“…They play an important role in growth and maintain anabolic effects in animals. Insulin and IGF-I inhibit overall protein breakdown, the degradation of myofibrillar proteins (Sacheck et al, 2004), and the expression of muscle-specific ubiquitin ligases (atrogin-1/MAFbx and MuRF1) (Sacheck et al, 2004;Tesseraud et al, 2007;Dupont et al, 2008;Saneyasu et al, 2017;Nakashima et al, 2017). Furthermore, insulin and IGF-I inhibit autophagy in skeletal muscle and muscle cells (Seiliez et al, 2010;Naito et al, 2013;Suryawan and Davis, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids

Nakashima

Ishida

2018

J. Poult. Sci.

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Autophagy, an intracellular bulk protein degradation system in skeletal muscle, is increased under catabolic conditions resulting in muscle atrophy. This study aimed to investigate the effects of insulin, insulin-like growth factor (IGF)-I, and amino acids on autophagy (LC3-II content and expression of autophagy-related genes) in chick myotubes. Chick myotubes were incubated with insulin (1 μg/ml), IGF-I (100 ng/ml), and amino acids for 3 h. The LC3-II content, an index of autophagosome formation, and mRNA expression of LC3B and GABARAPL1 were significantly decreased by insulin. The LC3-II content, but not mRNA expression of autophagy-related genes, was also significantly decreased by IGF-I. The LC3-II content and LC3B mRNA level were also significantly decreased by amino acids. The mRNA expression of atrogin-1/MAFbx, a muscle-specific ubiquitin ligase, was also significantly decreased by insulin, IGF-I, and amino acids in chick myotubes. These results indicated that insulin, IGF-I, and amino acids regulate autophagy as well as the ubiquitin-proteasome proteolytic pathway in chick myotubes.

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Section: Resultssupporting

confidence: 62%

Section: Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids

Nakashima

Ishida

2018

J. Poult. Sci.

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“…Insulin-like growth factor-1 (IGF-1) is a key regulator of muscle development and metabolism in chickens. For example, IGF-1 increased cell protein content in chicken myotube (Nakashima et al, 2017) and promoted chicken myoblast proliferation (Yu et al, 2015). Tomas et al (1998) reported enhancement of growth and feed efficiency of broiler chickens receiving exogenous IGF-1.…”

Section: Introductionmentioning

confidence: 99%

Role of Insulin-like Growth Factor-1 in the Central Regulation of Feeding Behavior in Chicks

et al. 2019

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Insulin-like growth factor-1 (IGF-1) is a key regulator of muscle development and metabolism in chickens. Recently, we have demonstrated that intracerebroventricular administration of IGF-1 significantly decreased food intake in broiler chicks. However, the molecular mechanisms underlying the IGF-1-induced anorexia and the anorexigenic effect of IGF-1 in different strains of commercial chicks have not been investigated. Neuropeptide Y (NPY, a hypothalamic orexigenic neuropeptide), agouti-related protein (AgRP, a hypothalamic orexigenic neuropeptide), and proopiomelanocortin (POMC, the precursor of hypothalamic anorexigenic neuropeptides) play important roles in the regulation of food intake in both mammals and chickens. Evidence shows that several cell signaling pathways in the hypothalamus are involved in regulating the feeding behavior of mammals. In the present study, we first investigated the effects of IGF-1 on the expression of appetite-regulating neuropeptides and phosphorylation of signaling molecules in the hypothalamus of broiler chicks. Intracerebroventricular administration of IGF-1 significantly increased the mRNA levels of POMC, whereas the mRNA levels of NPY and AgRP were not significantly altered. IGF-1 also significantly induced the phosphorylation of v-Akt murine thymoma viral oncogene homolog 1 (AKT) in the hypothalamus of chicks, but did not influence the phosphorylation of forkhead box O1, S6 protein, AMP-activated protein kinase, and extracellular signal-regulated kinase 1/2. We also compared the effect of IGF-1 on food intake in broiler chicks (a hyperphagic strain of chickens) and layer chicks. Results demonstrated that the threshold of IGF-1-induced anorexia in broiler chicks was higher than that in layer chicks. Our observations suggest that hypothalamic POMC and AKT may be involved in the IGF-1-induced anorexigenic pathway and that high threshold of IGF-1-induced anorexia in broiler chicks might be one of the causes of hyperphagia in broiler chicks. Overall, it appears that IGF-1 plays important roles in the central regulation of feeding behavior in chicks.

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“…IGF-1 and GHR are strong growth regulators and have anabolic effects on protein and carbohydrate metabolism [ 55 ]. IGF-1 is a vital regulator for the development of muscle as it amplifies cell protein content in broiler myofiber [ 56 ] and stimulates broiler myoblast proliferation. Moreover, IGF-1 stimulates embryonic skeletal muscle growth, and skeletal myogenesis, and consequently improves chicken meat production [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Nano-Methionine supplementation in drinking water on growth performance, lipid metabolism, and related gene expression in broiler chicken

Shobokshy¹,

Samaha²,

Maksoud³

et al. 2022

J Adv Vet Anim Res

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Objectives: The study aimed to determine how Nano-Methionine (Nano-Meth) affected growth, lipid metabolism, and relative gene expression for acetyl-CoA carboxylase (ACC), fatty acid syn¬thase (FAS), growth hormone receptor (GHR), insulin-like growth factor receptor-1 (IGFR-1), myo¬statin (MSTN), and cholecystokinin (CCK) genes in broiler chickens. Materials and Methods: A total of 100 1-day-old broilers were randomly assigned into 2 groups: 1) the control group received drinking water without any supplements, and 2) the Nano-Meth group received 10 ml/l of 5% Nano-Meth starting from 1 day old until 35 days old (the end of the experiment). Results: Nano-Meth improved final body weight, weight gains, feed intake, and feed conver¬sion ratio. Compared to the control group, Nano-Meth significantly lowered the serum levels of triglyceride, cholesterol, very low-density lipoprotein, and low-density lipoprotein in chickens. Nano-Meth significantly increased the serum levels of total protein, albumin, high-density lipo-protein, and glucose more than the control group. Nano-Meth lowered the mRNA gene expression of ACC, FAS, MSTN, and CCK but increased that of GHR and IGFR-1. Conclusions: We concluded that supplementation with Nano-Meth enhances growth performance and decreases lipid accumulation in broiler chickens.

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Effects of Insulin-Like Growth Factor-I on the Expression of Atrogin-1/MAFbx in Chick Myotube Cultures

Cited by 13 publications

References 33 publications

Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids

Regulation of Autophagy in Chick Myotubes: Effects of Insulin, Insulin-Like Growth Factor-I, and Amino Acids

Role of Insulin-like Growth Factor-1 in the Central Regulation of Feeding Behavior in Chicks

Influence of Nano-Methionine supplementation in drinking water on growth performance, lipid metabolism, and related gene expression in broiler chicken

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