Effects of Inoculants Producing Antifungal and Carboxylesterase Activities on Corn Silage and Its Shelf Life against Mold Contamination at Feed-Out Phase

Abstract

The present study aimed to investigate effects of dual-purpose inoculants (antifungal and carboxylesterase activities) not only on corn silage quality, but also its shelf life against mold contamination at feed-out phase. Corn forage was ensiled for 252 d with different inoculants of the following: control (CON), Lactobacillus brevis 5M2 (5M), Lactobacillus buchneri 6M1 (6M), and mixture of 5M and 6M at 1:1 ratio (MIX). After ensiling, corn silage was contaminated with Fusarium graminearum. Silages applied inoculants had positive effects by increased organic acid and lactic acid bacteria, and decreased undesirable microbes. At feed-out phase, contamination of F. graminearum into corn silage had a negative effect on aerobic stability caused by increased growth of undesirable microbes. However, silages applied inoculants had positive effects by decreased undesirable microbes and extended lactic acid bacteria and aerobic stability. Generally, MIX silage presented better effects on organic acid production, rumen degradation, inhibition of undesirable microbes, and aerobic stability than 5M silage and 6M silage. The present study concluded that application of inoculants into corn silage had positive effects on fermentation characteristics and extended shelf life against mold contamination at feed-out phase. A mixed inoculant appeared to have better effects of antifungal and carboxylesterase than a single inoculant.

Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

“…Degrading lignocellulose could increase fiber digestibility in the rumen [4,8,13]. Again, Kim et al [13] reported that the Leuc.…”

“…Even though improved ensiling processes, bacterial additives are not always guaranteed to reduce contamination by undesirable microbes after silo open [1]. Recently, many researchers revealed that lactic acid bacteria (LAB) can produce antifungal activity to reduce contamination by undesirable microbes on silage, especially after silo open [2][3][4]. In addition, acetate and propionate produced by LAB inhibit the growth of fungi during ensiling through cell wall breakage and mitochondria apoptosis, respectively [5,6].…”

“…In addition, acetate and propionate produced by LAB inhibit the growth of fungi during ensiling through cell wall breakage and mitochondria apoptosis, respectively [5,6]. According to previous studies, the application of LAB producing antifungal activity can reduce the growth of undesirable microbes and increase the aerobic stability of silage [3,4]. On the other hand, some forages are characterized by high structural carbohydrate and low soluble carbohydrate, which reduce either ensiling quality or digestibility [7].…”

“…Several strains of LAB were reported to produce fibrinolytic enzymes such as esterase and xylanase, which could help to degrade structural carbohydrate and improve ruminal digestibility [8,9]. Application of LAB producing fibrinolytic activity on silage was reported to increase ruminal digestibility in many previous studies [4,8].…”

Application of Lactic Acid Bacteria (LAB) in Sustainable Agriculture: Advantages and Limitations

“…LAB strains are isolated from dairy products and control soil-borne pathogens. In addition, Lactobacillus buchneri isolated from corn silages showed antifungal activity against F. graminearum [ 34 ]. Hamed et al (2011) demonstrated that seed pre-treatment before planting with an LAB nutritive solution reduces the damping-off diseases [ 35 ].…”