This study was conducted to know effects of forage cutting height and inoculants application on chemical composition, fermentation characteristics and fatty acid profile of barley silage. Barley forage(Yuyeon hybrid) was harvested at two different cutting heights(5 vs. 15cm) and applied with or without Lactobacillus plantarum, and ensiled for 0, 2, 7, 28, 49 and 100days. On 0 to 49-d of ensiling, higher cutting height resulted rapid drop (p<0.05) in pH caused by higher lactate content. Crude protein (p<0.01) content of 100-d silage was decreased by inoculation, but increased by higher cutting height. However, neutral detergent fiber and acid detergent fiber contents were decreased (p<0.05) by both inoculation and cutting height. In vitro dry matter digestibility was improved by higher cutting height (p=0.01), while yeast and mold counts were reduced (p<0.0001). The C18:3n-3 content in barley silage was decreased (p=0.001) by inoculation, but increased (p=0.034) by higher cutting height. The DNA analysis indicated L. plantarum dominating fermentation in inoculated silages. The results showed that higher cutting height can improve silage quality in terms of increasing crude protein content and digestibility as well as reducing yeast and mold counts in barley silage.
This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.
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