Effects of Rosa canina fruit extract on neutrophil respiratory burst

Abstract

Respiratory burst leads polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS) such as superoxide anions (O(2)(o-)), hypochlorous acid (HOCl) and hydrogen peroxide (H(2)O(2)) which may possess deleterious effects for the organism. Rosa canina fruits are well known to contain a large amount of vitamin C which is antioxidant. This study was focused on the polyphenolics contained in rose hips to evaluate their antioxidative properties. We prepared a rose hip extract deprived of vitamin C. The extract contained mainly phenolics such as proanthocyanidins and flavonoids. We investigated its effects directly against (O(2)(o-)), HOCl and H(2)O(2) and investigated its effects on isolated PMN. For that, in vitro inflammatory conditions were reproduced by stimulating PMN with stimuli having different transductional pathways, in order to determine a possible mechanism of action. The results showed that the extract can inhibit ROS tested in acellular and cellular systems. The IC(50) obtained were 5.73 mg/L, 1.33 mg/L and 2.34 mg/L respectively for (O(2)(o-)), HOCl and H(2)O(2) in acellular experiments. For cellular experiments, the IC(50) were quite similar. Thus, the extract did not present an effect on PMN metabolism. Therefore, the antioxidative effects of Rosa canina are due not only to vitamin C but also to polyphenolics.

Fruit quality of dog rose seedlings (Rosa canina L)

“…koji sprečava pojavu skorbuta (Erçisli and Güleryüz, 1996). Nadalje, šipak ima anti-upalna (Larsen, et al, 2003.;Warholm, et al, 2003.;Winther, et al, 1999), antibakterijska (Ozturk Yilmaz i Ercişli, 2011) i antioksidativna svojstva (Daels-Rakotoarison, et al, 2002.;Gao, et al, 2000.;Halvorsen, et al, 2002) Plodovi se uglavnom ne konzumiraju u svježem stanju, već se koriste za proizvodnju marmelada, džemova, sokova, čajeva i sirupa (Ercişli, 1996). Osim mesa ploda, mogu se uporabiti i sjemenke i to za dobivanje ulja.…”

Genoprotective Effects of Aqueous Extracts of Rosa Canina L. Fruits on Ethyl Methanesulfonate-Induced DNA Damage in Drosophila Melanogaster

“…25 mg / ml of the dried rosehip extract inhibited 83.7 % lipid peroxidation in vitro [8]. Extracts of the rosehip reduced the formation of free radicals in the polynuclear neutrophil cells [9].…”

“…There are also a lot of mineral such as potassium, sodium, calcium, magnesium, iron, phosphorus, copper, zinc in the rosehip berries [7]. Therefore the rosehip plant (R.canina) has antioxidant properties [8,9] and protective effects against to DNA damage [10]. Studies which used different plants as curative are available.…”

Mutagenic biomonitoring of pirethroid insecticides in human lymphocyte cultures: Use of micronuclei as biomarkers and recovery byRosa caninaextracts of mutagenic effects

“…According to Serteser et al (2008), rosehip is a potent-free radical scavenger and a natural source of antioxidants. Rosehip plants have various phenolic compounds such as methyl gallate (Hvattum, 2002), protocatechuic acid (Khadem & Marles, 2010), and flavonoids (Daels-Rakotoarison et al, 2002). It was reported that there was a strong correlation between the phenolic compounds in R. canina and antioxidant activity (DaelsRakotoarison et al, 2002).…”