Genoprotective Effects of Aqueous Extracts of Rosa Canina L. Fruits on Ethyl Methanesulfonate-Induced DNA Damage in Drosophila Melanogaster

Abstract

Abstract. In this study, the possible genotoxic effects of ethyl methanesulfonate (EMS) which are one of alkylating agent and genoprotective effects of Rosa canina L. fruit water extract (RCwtr) was studied with Drosophila wing somatic mutation and recombination test (SMART). Five different application groups (distilled water, 1 mM EMS, 1% RCwtr, 3% RCwtr and 5% RCwtr) were formed with preliminary studies. 3-day-old transheterozygous larvae of mwh/flr 3 genotype of Drosophila melanogaster were fed chronically on the Drosophila instant medium (DIM) including the application groups. The wing slides of normal wing (mwh/flr 3 ) phenotype individuals were prepared and examined under light microscope (400X).As a result of experiments, the total clone frequencies of distilled water control group, 1 mM EMS, 1% RCwtr, 3% RCwtr and 5% RCwtr application groups were determined as 0. 3 ) bireylerin kanat preparatları hazırlanmış ve ışık mikroskobunda incelenmiştir (400X).Deneylerin sonucunda saf su kontrol grubu, 1 mM EMS, %1 RCsu, %3 RCsu ve %5 RCsu uygulama gruplarının toplam klon frekansları sırasıyla 0.15, 3.55, 2.58, 2.78 ve 2.20 olarak belirlenmiştir. Saf su kontrol grubu ve 1 mM EMS uygulama grupları arasındaki fark istatistiksel olarak önemlidir (P<0.05). RCsu uygulama gruplarından elde edilen sonuçlara göre ise, 1 mM EMS uygulama grubu ile karşılaştırıldığında her grubun toplam klon frekansı azalmıştır. 1mM EMS ve RCsu uygulama grupları arasındaki farkların istatistiksel olarak oldukça önemli olduğu bulunmuştur (P<0.05). Bulgular, Rosa canina L. bileşenlerinin doğal bir genomik koruyucu ürün olarak büyük bir potansiyele sahip olduğunu göstermektedir.

Somatic mutations and recombination test in Drosophila melanogaster used for investigating the genotoxicity of some food additives

“…Experiments in the present study were simultaneously conducted in the application groups consisting of experimental and control groups. Distilled water was used in the negative control group and, in literature, 1 mM concentration of EMS (Kasımoğlu & Uysal 2016), which was used in the positive control group, was stated to have a mutagenic effect. All of the food colorings used in the experiment groups dissolved in distilled water.…”

Investigation of Mutagenic Effects of Pea Green and Anti-carcinogenic Effects of Several Plants Extracts in Drosophila Melanogaster Using Wing Spot

“…Experiments of the application groups consisting of experimental, treatment and control groups in the study were conducted simultaneously. Distilled water was used in the negative control group and EMS (Kasımoğlu 2016) is stated in the literature that it has a mutagenic effect, was used at 1 mM concentration in the positive control group. The food coloring and the plant extracts used in the application groups were dissolved in distilled water.…”