Effects of Psoralea corylifolia L. seed extract on AGEs‑induced cell proliferation and fibrotic factor expression in mesangial cells

Abstract

Diabetic nephropathy is a microvascular complication of diabetes that is characterized by mesangial expansion and thickening of the glomerular basement membrane. The production of advanced glycation end products (AGEs) increases in diabetic patients. Activation of the receptor of AGE (RAGE) signaling pathway induces mesangial expansion via the reactive oxygen species (ROS)-mediated production of pro-inflammatory and extracellular matrix molecules. The Psoralea corylifolia L. seed (PCS) is a widely used herbal medicine with various biological activities. The current study investigated the effect of PCS extract on mesangial cell proliferation and the RAGE signaling pathway in SV40 MES 13 cells. SV40 MES 13 cells were harvested after treatment with various concentrations of PCS extract at 10 µg/ml AGEs for 24 h. The results revealed that the PCS extract inhibited AGEs-induced mesangial cell proliferation and cyclin protein expression in a concentration-dependent manner. In addition, the AGEs-induced expression of fibrotic factors, such as transforming growth factor β, fibronectin and collagen, was reduced in mesangial cells after exposure to the PCS extract. The PCS extract also reduced RAGE expression and inhibited the expression of its downstream signaling pathways, such as NADPH oxidase, intracellular ROS and phospho-NF-κB. In conclusion, the data suggested that the PCS extract attenuated AGEs-induced renal mesangial cell proliferation and fibrosis via the suppression of oxidative stress and the downregulation of inflammatory and fibrotic factor expression.

Advanced Glycation End Products-Induced Activation of Keratinocytes: A Mechanism Underlying Cutaneous Immune Response in Psoriasis

“…Supplementary to these previous findings, the current study further showed that AGEs upregulated the expression of K17 and resulted in the nuclear export and degradation of p27 KIP1 , leading to progressed cell cycle and ultimately the proliferation of keratinocytes. Multiple studies have demonstrated the pro-proliferative effects of AGEs on different types of cells like mesangial cells and aortic smooth muscle cells [25][26][27]. However, some other studies have claimed the inhibitory effects of AGEs on cell proliferation or cell cycle progression in ovarian granulosa cells and intestinal epithelial cells [58,59].…”

“…Given that AGEs are able to induce the proliferation of various types of cells [19,[25][26][27], we speculated that increased AGEs could contribute to the excessive proliferation of epidermal keratinocytes in psoriasis. To verify this, we first examined the growth of NHKs treated with BSA-AGEs via CCK8 assay and resazurin-reduction assay.…”

Green Synthesis of Copper‐oxide Nanoparticles and Evaluation of Its Therapeutic Efficacy

“…[ 40,41 ] Eclipta prostrata leaf extract mediated CuONPs synthesized and screened for its antimicrobial activity. [ 42 ] In this work, to study the antimicrobial activity of CuONPs, we used Prunella vulgaris (P. Vulgaris) , a perennial herbaceous plant of the labiaceae family. P. vulgaris , commonly known as self‐heal, has been used as a traditional medicinal plant in various parts of the world such as Asia, Europe, and North America.…”

Methylglyoxal is the main culprit to impairing neuronal function: mediated through tryptophan depletion

“…In chronic hyperglycemia, highly reactive metabolites produced during glycolysis are the main cause of impaired normal cell function, resulting in different types of chronic diseases [20]. Among them, methylglyoxal (MGO) is the most reactive dicarbonyl substance, it usually binds to the amino acids, especially, arginine and lysine, to form advanced glycation end-products (CEL, MGH1, etc) which ultimately deteriorate normal protein functions [21]. MGO has been reported to play a critical role in different central nervous system (CNS) cognitive functions, particularly anxiety, stress, depression, and neurodegenerative diseases [22].…”