Effects of Hibiscus sabdariffa Linn. fruit extracts on α-glucosidase enzyme, glucose diffusion and wound healing activities

Shadhan, Raheem Mohssin; Bohari, Siti Pauliena Mohd

doi:10.1016/j.apjtb.2017.01.023

Cited by 15 publications

(14 citation statements)

References 21 publications

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“…In vitro and in vivo antidiabetic effects of H. sabdariffa has been reported in previous studies (Ademiluyi & Oboh, 2013;Gondokesumo et al, 2017;Peng et al, 2011;Rasheed et al, 2018;Shadhan & Bohari, 2017;Su, Li, Yang, Hou, & Ye, 2018). Consistently, we observed that aqueous calyx extract of H. sabdariffa inhibited α-amylase (IC 50 : 490.79 μg/ml) and α-glucosidase (IC 50 : 495.31 μg/ml) and lowered blood glucose of rats (Table 1).…”

Section: Gallic and Protocatechuic Acids From H Sabdariffa Inhibitsupporting

confidence: 90%

“…Inhibitory activities of various extracts of H. sabdariffa calyx against α‐amylase and α‐glucosidase have been reported (Ademiluyi & Oboh, ; Gondokesumo, Kusuma, & Widowati, ; Shadhan & Bohari, ). In this study, we provide evidence showing that gallic and protocatechuic acids isolated from H. sabdariffa calyx have α‐amylase and α‐glucosidase inhibitory activities in both in vitro enzyme inhibition assays and in silico molecular docking simulations.…”

Section: Introductionmentioning

confidence: 99%

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Antidiabetic activity‐guided isolation of gallic and protocatechuic acids fromHibiscus sabdariffacalyxes

et al. 2019

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We isolated and identified gallic and protocatechuic acids as the antidiabetic principles in Hibiscus sabdariffa using solvent extraction, column chromatographic fractionation, and nuclear magnetic resonance (NMR) spectroscopy. Ethylacetate fraction of the aqueous extract of H. sabdariffa inhibited α‐amylase and α‐glucosidase with IC50 of 411.73 and 433.93 μg/ml, respectively. Furthermore, fractions I and II obtained from column chromatography inhibited α‐amylase with IC50 of 27.03 and 20.12 μg/ml, and α‐glucosidase with IC50 of 24.30 and 22.29 μg/ml, respectively. In addition, the principles reduced the serum glucose and lipid peroxide levels of diabetic rats and with an improvement in the rat lipid profiles and antioxidant defenses. Fractions I and II were identified as protocatechuic acid and gallic acid, respectively, using 1H and 13C NMR. Protein–ligand docking showed that these compounds form multiple favorable interactions with the active‐site residues of the two glycosidases. Overall, protocatechuic and gallic acids emerge as natural antidiabetic agents. Practical applications Hibiscus sabdariffa (Zoborodo) is a refreshment drink for ceremonial gatherings in Nigeria. Also, its pharmacological use includes diabetes, hypertension, hyperlipidemia, metabolic syndrome, and hepatoprotection. The consumption of this food drink could improve diabetes, hypertension, dyslipidemia, metabolic syndrome, and liver disease. Furthermore, the inhibition of α‐amylase and α‐glucosidase could prevent diabetic complications associated with postprandial glucose. Developing the extract of H. sabdariffa calyx as food supplement could be used in managing diabetes and its associated complications such as dyslipidemia, hypertension, and metabolic syndrome.

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Section: Gallic and Protocatechuic Acids From H Sabdariffa Inhibitsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Antidiabetic activity‐guided isolation of gallic and protocatechuic acids fromHibiscus sabdariffacalyxes

et al. 2019

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“…The dried leaves were ground by a standard blender to get a fine powder. The leaves powder was kept in a sealed container in a dry and cool place to be used throughout the study period [16].…”

Section: Plant Materialsmentioning

confidence: 99%

“…The filtered methanol extract was evaporated using a rotary evaporator (EYELA-Tokyo Rikakikai Company, Tokyo, Japan) and dried using a freeze dryer (Martin Christ Company, Osterode am Harz, Germany) to obtain dark crude A. precatorius extract [16,17]. The percentage yield of the crude methanol extract obtained was 1.86 grams, and kept at 4 °C for further use.…”

Section: Preparation Of a Precatorius Leaves Extractmentioning

confidence: 99%

“…Approximately 20 µL different concentrations of A. precatorius extract were mixed with 50 µL of 50 mM phosphate buffer, followed by 10 µL of α-glucosidase from S. cerevisiae (1 U/mL). These samples were further incubated for 5 min at 37 °C [16]. A 20 µl of p-nitrophenyl alpha-Dglucopyranoside (PNP-G) was then added to the mixture as the substrate.…”

Section: Alpha-glucosidase Inhibition Assaymentioning

confidence: 99%

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in vitro Study of Antidiabetic Effect of Abrus precatorius Methanol Leaves Extract against Glucose Absorption

Al-Moalemi

Altowayti

Bohari

2020

International Journal of Life Sciences and Biotechnology

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Diabetes mellitus is a common chronic systemic disorder characterised by hyperglycaemia as a standard feature. A traditional plant known as Abrus precatorius (AP) has been used for the treatment of type II diabetes mellitus in Malaysia. The potential of the 80% methanol leaves extract of A. precatorius has been tested for its α-glucosidase inhibition using α-glucosidase inhibitory assay and glucose diffusion activity using an in vitro model. It was observed that the methanol leaves extract of A. precatorius exhibited a high α-glucosidase inhibition at the concentrations of 25 and 50 mg/mL (65.4% and 84.6%), respectively, but low inhibition at the concentration of 6.25 to 12.5 mg/mL (25% and 28.2%) when compared to control. And it slightly affected the glucose diffusion at the concentration of 50 mg/mL (9.5%) within 24 h compared to the control group. These indicated that the methanol leaves extract of A. precatorius is capable of inhibiting α-glucosidase activity, besides halting glucose diffusion activity by delaying the glucose absorption in the gut.

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In vitro antidiabetic, antioxidant activities and GC–MS analysis of Rhynchostylis Retusa and Euphorbia Neriifolia leaf extracts

et al. 2021

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Effects of Hibiscus sabdariffa Linn. fruit extracts on α-glucosidase enzyme, glucose diffusion and wound healing activities

Cited by 15 publications

References 21 publications

Antidiabetic activity‐guided isolation of gallic and protocatechuic acids fromHibiscus sabdariffacalyxes

Antidiabetic activity‐guided isolation of gallic and protocatechuic acids fromHibiscus sabdariffacalyxes

in vitro Study of Antidiabetic Effect of Abrus precatorius Methanol Leaves Extract against Glucose Absorption

In vitro antidiabetic, antioxidant activities and GC–MS analysis of Rhynchostylis Retusa and Euphorbia Neriifolia leaf extracts

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