in vitro Study of Antidiabetic Effect of Abrus precatorius Methanol Leaves Extract against Glucose Absorption

Abstract

Diabetes mellitus is a common chronic systemic disorder characterised by hyperglycaemia as a standard feature. A traditional plant known as Abrus precatorius (AP) has been used for the treatment of type II diabetes mellitus in Malaysia. The potential of the 80% methanol leaves extract of A. precatorius has been tested for its α-glucosidase inhibition using α-glucosidase inhibitory assay and glucose diffusion activity using an in vitro model. It was observed that the methanol leaves extract of A. precatorius exhibited a high α-glucosidase inhibition at the concentrations of 25 and 50 mg/mL (65.4% and 84.6%), respectively, but low inhibition at the concentration of 6.25 to 12.5 mg/mL (25% and 28.2%) when compared to control. And it slightly affected the glucose diffusion at the concentration of 50 mg/mL (9.5%) within 24 h compared to the control group. These indicated that the methanol leaves extract of A. precatorius is capable of inhibiting α-glucosidase activity, besides halting glucose diffusion activity by delaying the glucose absorption in the gut.

In-Silico Analysis And Antidiabetic Effect Of Α-Glucosidase Inhibitory Of Active Compounds Of Pterocarpus Marsupium

“…When carbohydrates are broken down by the enzyme alpha-amylase (glucose), disaccharides are broken down or converted to monosaccharides 14,15 . These inhibitions might postpone the intestinal breakdown of carbohydrates, which would assist to reduce T2DM 16,17 . One of the therapeutic approaches being researched for the treatment of diabetes is the inhibition of glucosidase and amylase activity, and herbal plants are providing a therapeutic approach to treat diabetic Mellitus (DM) because they are a rich source of various bioactive compounds that have either directly or indirectly been used in the treatment of various diseases and ailments.…”