Effects of green-synthesized silver nanoparticles on lung cancer cells in vitro and grown as xenograft tumors in vivo

Abstract

Silver nanoparticles (AgNPs) have now been recognized as promising therapeutic molecules and are extending their use in cancer diagnosis and therapy. This study demonstrates for the first time the antitumor activity of green-synthesized AgNPs against lung cancer in vitro and in vivo. Cytotoxicity effect was explored on human lung cancer H1299 cells in vitro by MTT and trypan blue assays. Apoptosis was measured by morphological assessment, and nuclear factor-κB (NF-κB) transcriptional activity was determined by a luciferase reporter gene assay. The expressions of phosphorylated stat3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. AgNPs showed dose-dependent cytotoxicity and stimulation of apoptosis in H1299 cells. The effects on H1299 cells correlated well with the inhibition of NF-κB activity, a decrease in bcl-2, and an increase in caspase-3 and survivin expression. AgNPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of AgNPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early-stage intervention.

Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (<em>Garcinia mangostana</em>) pericarp waste extracts

“…Many other researchers also have reported the cytotoxicity of green-synthesized AgNPs on lung cancer cells. 29,30 Wang et al 29 reported green-synthesized AgNPs by Dendropanax morbifera leaf extract, and the AgNPs showed potent cytotoxicity toward the A549 cells. The extract of Dimocarpus 33 Therefore, asymmetric dumbbell-shaped …”

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

“…In this regard, nanotechnology-based therapy for cancer is the most promising field for generating new application in drug delivery with minimal side effects (Hasadsri et al 2009, Rani et al 2012. Various nanovectors and nanoconjugate such as polymeric conjugates, synthetic organic nanoparticles like dendrimers (Karimi et al 2014), lipids such as liposomes and micelle (Dicheva andKoning 2014, Gupta et al 2015), carbon nanotubes, inorganic nanoparticles (He et al 2016) are gaining interest as drug delivery platforms (Bhattacharyya et al 2011, Sivasankar andKumar 2010). Nowadays, nanoparticles for controlled local drug delivery are suitable tools to improving serum solubility of the drugs, prolonging the drug release rate, improving the therapeutic index and pharmacokinetics of the drugs in contrast to the free drug counterparts (Mello andRicci-J unior 2011, Singh et al 2011).…”

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“…Ag-NPs significantly suppressed the H1299 tumor growth in a xenograft severe combined immunodeficient (SCID) mouse model. The results demonstrate the anticancer activities of Ag-NPs, suggesting that they may act as potential beneficial molecules in lung cancer chemoprevention and chemotherapy, especially for early -stage intervention 179 .…”