Effects of freezing on bone histological morphology

Andrade, Miguel Gustavo Setúbal; Sá, Camila Neves; Marchionni, Antônio Márcio Teixeira; Bittencourt, Thereza Cristina Borio dos Santos Calmon de; Sadigursky, Moysés

doi:10.1007/s10561-008-9065-4

Cited by 30 publications

(21 citation statements)

References 25 publications

(30 reference statements)

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“…This proved to be an adequate method for allografts, preserving the osteoconductive and structural properties of the graft tissue as a scaffold for vessel and cell proliferation, as demonstrated by partial filling of osteocyte lacunae, early-stage bone remodeling, and incorporation at the graft-host interface. The use of other methods, such as freeze-drying, demineralization, gamma irradiation, ethylene oxide, or hydrogen peroxide, can jeopardize the structural properties of the donor tissue, which are of the utmost importance in onlay grafting 9,12,13,31-37…”

Section: Discussionmentioning

confidence: 99%

Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Valiati

Paes²,

Moraes³

et al. 2012

Int. J. Med. Sci.

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Objective To assess the effect of low-level laser therapy (LLLT) on the incorporation of deep-frozen block allografts in a rabbit model.Background Data Studies have shown that LLLT has beneficial effects on tissue repair and new bone formation.Methods Bone tissue was harvested from two rabbits, processed by deep-freezing and grafted into the calvaria of 12 animals, which were then randomly allocated into two groups: experimental (L) and control (C). Rabbits in group L were irradiated with an aluminum gallium arsenide diode laser (AlGaAs; wavelength 830 nm, 4 J/cm2), applied to four sites on the calvaria, for a total dose of 16 J/cm2 per session. The total treatment dose after eight sessions was 128 J/cm2. Animals were euthanized at 35 (n = 6) or 70 days (n = 6) postoperatively.Results Deep-freeze-processed block allografts followed by LLLT showed incorporation at the graft-host interface, moderate bone remodeling, partial filling of osteocyte lacunae, less inflammatory infiltrate in the early postoperative period, and higher collagen deposition than the control group.Conclusion Optical microscopy and scanning electron microscopy showed that allograft bone processed by deep-freezing plus LLLT is suitable as an alternative for the treatment of bone defects. Use of the deep-freezing method for processing of bone grafts preserves the structural and osteoconductive characteristics of bone tissue.

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Section: Discussionmentioning

confidence: 99%

Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Valiati

Paes²,

Moraes³

et al. 2012

Int. J. Med. Sci.

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“…Although this tissue had been subjected to previous freeze-thaw cycles, which may compromise bone tissue and cellular ultrastructure (e.g. nuclei and osteocytes; Andrade et al, 2008), these changes are irrelevant for the scale of our observations and osteocytes are lacking from the bone of these species.…”

Section: Histologymentioning

confidence: 99%

Feeding in billfishes: inferring the role of the rostrum from a biomechanical standpoint

Habegger

Dean

Dunlop

et al. 2015

Journal of Experimental Biology

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Perhaps the most striking feature of billfishes is the extreme elongation of the premaxillary bones forming their rostra. Surprisingly, the exact role of this structure in feeding is still controversial. The goal of this study is to investigate the use of the rostrum from a functional, biomechanical and morphological standpoint to ultimately infer its possible role during feeding. Using beam theory, experimental and theoretical loading tests were performed on the rostra from two morphologically different billfish, the blue marlin (Makaira nigricans) and the swordfish (Xiphias gladius). Two loading regimes were applied (dorsoventral and lateral) to simulate possible striking behaviors. Histological samples and material properties of the rostra were obtained along their lengths to further characterize structure and mechanical performance. Intraspecific results show similar stress distributions for most regions of the rostra, suggesting that this structure may be designed to withstand continuous loadings with no particular region of stress concentration. Although material stiffness increased distally, flexural stiffness increased proximally owing to higher second moment of area. The blue marlin rostrum was stiffer and resisted considerably higher loads for both loading planes compared with that of the swordfish. However, when a continuous load along the rostrum was considered, simulating the rostrum swinging through the water, swordfish exhibited lower stress and drag during lateral loading. Our combined results suggest that the swordfish rostrum is suited for lateral swiping to incapacitate their prey, whereas the blue marlin rostrum is better suited to strike prey from a wider variety of directions.

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“…A few studies reported successful preservation of tissue functionality including the structure of collagen and elastin in aortic and pulmonary valves, and electrochemical and biomechanical properties of articular cartilage [6, 19]. However, there are still many other studies reporting changes of these properties [18, 20–22]. Mechanistic understanding of changes in ECM structure during freezing and thawing is required to address this challenge.…”

Section: Introductionmentioning

confidence: 99%

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

Seawright

Özçelikkale

Dutton

et al. 2013

Journal of Biomechanical Engineering

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During cryopreservation, ice forms in the extracellular space resulting in freezing-induced deformation of the tissue, which can be detrimental to the extracellular matrix (ECM) microstructure. Meanwhile, cells dehydrate through an osmotically driven process as the intracellular water is transported to the extracellular space, increasing the volume of fluid for freezing. Therefore, this study examines the effects of cellular presence on tissue deformation and investigates the significance of intracellular water transport and cell-ECM interactions in freezing-induced cell-fluid-matrix interactions. Freezing-induced deformation characteristics were examined through cell image deformetry (CID) measurements of collagenous engineered tissues embedded with different concentrations of MCF7 breast cancer cells versus microspheres as their osmotically inactive counterparts. Additionally, the development of a biophysical model relates the freezing-induced expansion of the tissue due to the cellular water transport and the extracellular freezing thermodynamics for further verification. The magnitude of the freezing-induced dilatation was found to be not affected by the cellular water transport for the cell concentrations considered; however, the deformation patterns for different cell concentrations were different suggesting that cell-matrix interactions may have an effect. It was therefore determined that intracellular water transport during freezing was insignificant at the current experimental cell concentrations; however, it may be significant at concentrations similar to native tissue. Finally, the cell-matrix interactions provided mechanical support on the ECM to minimize the expansion regions in the tissues during freezing.

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Effects of freezing on bone histological morphology

Cited by 30 publications

References 25 publications

Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Feeding in billfishes: inferring the role of the rostrum from a biomechanical standpoint

Role of Cells in Freezing-Induced Cell-Fluid-Matrix Interactions Within Engineered Tissues

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