The aim of the present investigation was to evaluate the angiogenesis on dorsal cutaneous wounds in a rodent model treated with λ660 nm laser light. New vessel formation is a multistep process involving vessel sprouting, endothelial cell migration, proliferation and tube formation. Although several in vivo studies have shown that laser phototherapy influences tissue repair, a fully understanding of angiogenesis mechanisms are not yet known. Twenty-four young adult male Wistar rats weighing between 200 and 250 g were used. Under general anesthesia, one excisional wound was created on the dorsum of each animal and they were randomly distributed into two groups: one control and one treated with laser (λ660 nm, 16 mW, 10 J/cm2). Each group was subdivided into three subgroups according to the animal death timing (2, 4 and 6 days). Laser irradiation started immediately after surgery and was repeated every other day during the experiment and marked with Sirius Red, specific for collagen, and immunomarked with anti-TGF-β and anti-von Willebrand factor. Marked sections underwent histological analysis by light microscopy and the mean area of the wound of each animal was calculated and analyzed by ANOVA and Tukey's test (α=0.05). Although at some death periods, collagen expression and number of blood vessels on irradiated animals were higher than in the control ones, no significant differences were found at any time in relation to TGF-β expression (p>0.05). It was concluded that laser treatment (λ660 nm) contributed to increase angiogenesis.
LLLT alone accelerates post-surgical tissue repair and reduces edema and the polymorphonuclear infiltrate even in the presence of dexamethasone.
Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue. Although this technique is considered safe, there is little information about the morphological modifications that the medullary and cortical portions of bone suffer after freezing. Hence, the aim of this study was to investigate the morphology of bone tissue after freezing under different temperatures and periods. Twelve rabbits were used to analyze the effects of two temperatures, -20 degrees C and -70 degrees C, during four periods of time: 30, 60, 90, 120 days. Tissues were analyzed by HE, picro-sirius stains and also by Feulgen's reaction, through qualitative and morphometric ways, considering the area occupied by cells and nuclei, medullary and cortical portions, as well as by collagen expression at cortical. The differences among the treatments were analyzed by Tukey s test, at 5% significance level. Bone freezing increased cellular and nuclear areas at cancellous bone and diminished nuclear area at the cortical bone. Cortical bone collagen suffered denaturation proportionally to temperature decrease and to freezing duration. These alterations compromised the morphology of tissues after 90 or 120 days of freezing at the temperature of -70 degrees C. Cells necrosed during freezing, contributing to reduce bone antigenicity.
A esclerodermia sistêmica é uma doença dermatológica que pode afetar os tecidos orais e periorais. Sua etiologia é desconhecida sendo associada a um mecanismo autoimune. A doença acomete preferencialmente mulheres negras, entre 30 e 50 anos de idade, e se caracteriza por modificações da microcirculação causando fibrose e obliteração das veias da pele, pulmão, trato gastrointestinal, rins e coração. O fenômeno de Raynaud e edema das mãos e dedos são sinais característicos da doença. As manifestações orofaciais incluem aumento do espaço do ligamento periodontal, limitação da função mastigatória, dificuldade no processo de reparo, fibrose da pele, redução da abertura bucal, entre outros. O objetivo deste trabalho é relatar o caso clínico de uma paciente portadora de Esclerodermia Sistêmica, visando auxiliar o cirurgião-dentista a identificar os sinais e sintomas característicos dessa alteração sistêmica que pode acometer os pacientes.
Introduction: The ozone therapy is a bio-oxidation therapy, based on a gaseous mixture with oxygen and ozone. It can be considered an alternative therapeutic tool the treat- ment of many systemic and locals diseases. It induces acute oxygenation stress and is not deleterious, which allows the restoration of oxidation and reduction balance. Within the therapeutic effects, it has been associated to the improvement metabolism and the peripheral tissue oxygenation. In addition, ozone is an important antimicrobial agent against bacteria, fungi, protozoa and viruses. In Dentistry, the ozone therapy has been used in several clinical situations due to its mechanisms of action. Objectives: The pur- pose of this literature review is to discuss the chemical and physical properties of ozone and its mechanisms of action in tissue repair. Methods: It is a narrative review based on research articles searched in Pubmed and LILACS database, from December 2015 to May 2016. The following descriptors in English: “ozone”, “oxidative stress” and “tissue repair” used. Finally, 36 articles were included in this narrative literature review. Results and Conclusion: The use of ozone as treatment seems to be promising in health areas, including dentistry due to its biological and biochemical properties. However, there is a need for more methodologically adequate studies so it can be safely and efficiently applied.
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