Effects of Extracts Derived from Roots and Stems of Chelidonium majus L. on Oxidative Stress Biomarkers in the Model of Equine Plasma

Stefanowski, Nataniel

doi:10.15414/ainhlq.2021.0018

Cited by 4 publications

(2 citation statements)

References 43 publications

(46 reference statements)

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“…Both root and stalk extracts of CM collected from rural areas caused a statistically significant reduction in ceruloplasmin levels. These in vitro studies indicate that extracts from this plant are a significant source of natural antioxidants that could prevent the progression of various disorders caused by oxidative stress (Stefanowski et al, 2021).…”

Section: Introductionmentioning

confidence: 85%

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Stefanowski¹

2022

Agrobiodivers Improv Nutr health life Qual

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Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives belonging to the Papaveraceae family collected from the northern part of Poland using a muscle tissue model of rainbow trout (Oncorhynchus mykiss Walbaum). Therefore, in the present study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), protein oxidative modification carbonyl derivative content, total antioxidant capacity (TAC)] were used to evaluate the antioxidant activity of stalk and root extracts of Chelidonium majus L. (CM) at doses of 5 mg.mL -1 , 2.5 mg.mL -1 , 1.25 mg.mL -1 , and 0.63 mg.mL -1 . Muscle tissue homogenates of rainbow trout were used in this study. Phosphate buffer was used as a positive control. After incubation of the mixture at 25 °C for 2 h with continuous mixing, samples were used for biochemical studies. Results of our study revealed that a dose of CM extracts of 0.63 mg.mL -1 showed the highest antioxidant activity in the muscle tissue of rainbow trout. The extracts derived mainly from the roots of CM collected from rural areas were effective in reducing the levels of oxidative stress biomarkers by reducing lipid peroxidation markers, which may suggest that the active substances such as alkaloids (chelidonine, sanguinarine, berberine), flavonoids, phenols in these plants can effectively protect the membrane structures in muscle cells of salmonids. We also observed statistically significant reductions in levels of both aldehydic and ketonic derivatives of oxidatively modified proteins in muscle tissue of rainbow trout after incubation with CM extracts at this dose compared to the controls. The comparison of these results shows that CM extracts can effectively inhibit protein damage by scavenging free radicals and/or activation of antioxidant defenses. The secondary metabolites of CM, i.e. polyphenols and alkaloids, are most likely responsible for this effect. Using doses of 5 mg.mL -1 , 2.5 mg.mL -1 , and 1.25 mg.mL -1 of both root and stalk extracts in vitro study, statistically significant increases in levels of TBARS and OMP were observed. Screening of species belonging to the family Papaveraceae for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of diseases caused by oxidative stress in human and veterinary medicine.

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Section: Introductionmentioning

confidence: 85%

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Stefanowski¹

2022

Agrobiodivers Improv Nutr health life Qual

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“…However, an excessive amount of ROS and RNS results in the oxidation of biological molecules such as lipids, proteins, and DNA. Oxidative stress has been reported in kidney disease, due to both antioxidant depletions as well as increased ROS production [22,23].…”

mentioning

confidence: 99%

Biomarkers of Oxidative Stress in the Muscle Tissue of Rainbow Trout (Oncorhynchus Mykiss Walbaum) After in Vitro Treatment by Extracts Derived From Stalks and Roots of Greater Celandine (Chelidonium Majus L.)

Stefanowski

Kurhaluk

Tkachenko

et al. 2021

STBIAS

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Consistent with our previous studies, we continue to evaluate the antioxidant potential of Greater celandine (Chelidonium majus L), a representative of the Papaveraceae family, collected from northern Poland using the model of muscle tissue of rainbow trout (Oncorhynchus mykiss Walbaum). Therefore, in the present study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), protein oxidative modification carbonyl derivative content, total antioxidant capacity (TAC)] were used to evaluate the antioxidant activity of extracts (final concentration 5 mg/mL) derived from stems and roots of C. majus. Rainbow trout muscle tissue was used in this study. Phosphate buffer was used as a positive control (blank). The results of the current study showed that stem and root extracts exhibited cytotoxic effects on cellular structures of muscle tissue by increasing the level of the lipid peroxidation biomarkers. These results suggest the possibility of using C. majus extract at 5 mg/mL as a source of pro-oxidant compounds and warrant further studies to evaluate their therapeutic potential. Levels of aldehydic and ketonic derivatives of oxidatively modified proteins and total antioxidant capacity were not significantly changed after in vitro incubation with the extracts derived from stalks and roots of C. majus. Screening of species of the family Papaveraceae for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of some diseases, as well as in the prevention and treatment of some disorders in medicine and veterinary.

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Dose-Dependent Alterations in the Biomarkers of Lipid and Protein Oxidation in the Muscle Tissue of Rainbow Trout (Oncorhynchus Mykiss Walbaum) After in Vitro Treatment by Extracts of Great Celandine (Chelidonium Majus L.)

Stefanowski

Tkachenko

Kurhaluk

et al. 2022

STBIAS

Self Cite

View full text Add to dashboard Cite

Consistent with our previous studies, we continue to evaluate the antioxidant potential of representatives of the Papaveraceae family collected from the northern part of Poland on the model of muscle tissue of rainbow trout. Therefore, in the current study, oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins (OMP), and total antioxidant capacity (TAC)] were used to evaluate the antioxidant activity of extracts derived from stalks and roots of great celandine (Chelidonium majus L., CM) at a final dose of 5 mg/mL, 2,5 mg/mL, 1,25 mg/mL and 0,63 mg/mL. Homogenate of muscle tissues derived from rainbow trout was used in this in vitro study. Phosphate buffer was used as a positive control (blank). After incubation of the mixture at 25°C for 120 min with continuous mixing, samples were used for biochemical studies. Our studies have shown that the use of extracts at a final dose of 5 mg/ml and 2.5 mg/ml resulted in a statistically significant increase of lipid peroxidation biomarkers (TBARS levels) in the muscle tissue of rainbow trout. The final dose of extract 1.25 mg/ml caused a statistically significant increase in the levels of aldehydic and ketonic derivatives of OMP, and this is reflected when measuring the levels of TAC. On the other hand, the use of extracts at a final dose of 0.63 mg/ml derived from both roots and stems of CM resulted in statistically significant reduced levels of TBARS, as well as aldehydic and ketonic derivatives of OMP in the muscle tissue of rainbow trout after in vitro incubation. The comparison of these results showed that CM extracts can effectively inhibit the production of oxidatively modified carbonyls by scavenging free radicals. The secondary metabolites of CM, i.e. polyphenols, are most likely responsible for this effect. Screening of species of the family Papaveraceae for other biological activities, including antioxidant activity, is essential and may be effective in the search for preventive measures in the pathogenesis of some diseases, as well as in the prevention and treatment of some disorders in veterinary and medicine. Keywords: rainbow trout (Oncorhynchus mykiss Walbaum), muscle tissue, oxidative stress, 2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins (OMP), total antioxidant capacity (TAC).

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Effects of Extracts Derived from Roots and Stems of Chelidonium majus L. on Oxidative Stress Biomarkers in the Model of Equine Plasma

Cited by 4 publications

References 43 publications

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Dose-Dependent Changes in the Levels of Oxidative Stress Biomarkers in the Muscle Tissue of Rainbow Trout (Oncorhynchus mykiss Walbaum) after In Vitro Treatment by Extracts Derived from Stalks and Roots of Great Celadine (Chelidonium majus L.)

Biomarkers of Oxidative Stress in the Muscle Tissue of Rainbow Trout (Oncorhynchus Mykiss Walbaum) After in Vitro Treatment by Extracts Derived From Stalks and Roots of Greater Celandine (Chelidonium Majus L.)

Dose-Dependent Alterations in the Biomarkers of Lipid and Protein Oxidation in the Muscle Tissue of Rainbow Trout (Oncorhynchus Mykiss Walbaum) After in Vitro Treatment by Extracts of Great Celandine (Chelidonium Majus L.)

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